Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090038499> ?p ?o ?g. }
- W2090038499 endingPage "438" @default.
- W2090038499 startingPage "421" @default.
- W2090038499 abstract "Extracellular flux (XF) analysis has become a mainstream method for measuring mitochondrial function in cells and tissues. Although this technique is commonly used to measure bioenergetics in intact cells, we outline here a detailed XF protocol for measuring respiration in permeabilized cells. Cells are permeabilized using saponin (SAP), digitonin (DIG) or recombinant perfringolysin O (rPFO) (XF-plasma membrane permeabilizer (PMP) reagent), and they are provided with specific substrates to measure complex I– or complex II–mediated respiratory activity, complex III+IV respiratory activity or complex IV activity. Medium- and long-chain acylcarnitines or glutamine may also be provided for measuring fatty acid (FA) oxidation or glutamine oxidation, respectively. This protocol uses a minimal number of cells compared with other protocols and does not require isolation of mitochondria. The results are highly reproducible, and mitochondria remain well coupled. Collectively, this protocol provides comprehensive and detailed information regarding mitochondrial activity and efficiency, and, after preparative steps, it takes 6–8 h to complete." @default.
- W2090038499 created "2016-06-24" @default.
- W2090038499 creator A5005305895 @default.
- W2090038499 creator A5015819680 @default.
- W2090038499 creator A5051328041 @default.
- W2090038499 date "2014-01-23" @default.
- W2090038499 modified "2023-10-17" @default.
- W2090038499 title "Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis" @default.
- W2090038499 cites W139355624 @default.
- W2090038499 cites W1507410890 @default.
- W2090038499 cites W1509512816 @default.
- W2090038499 cites W1544901029 @default.
- W2090038499 cites W1554777057 @default.
- W2090038499 cites W1559791712 @default.
- W2090038499 cites W1568876490 @default.
- W2090038499 cites W1591694509 @default.
- W2090038499 cites W1600619258 @default.
- W2090038499 cites W1896686934 @default.
- W2090038499 cites W1964126982 @default.
- W2090038499 cites W1965285497 @default.
- W2090038499 cites W1966159557 @default.
- W2090038499 cites W1968113458 @default.
- W2090038499 cites W1972847358 @default.
- W2090038499 cites W1974117358 @default.
- W2090038499 cites W1978048781 @default.
- W2090038499 cites W1978791657 @default.
- W2090038499 cites W1981791925 @default.
- W2090038499 cites W1981961082 @default.
- W2090038499 cites W1982330633 @default.
- W2090038499 cites W1984125550 @default.
- W2090038499 cites W1987219977 @default.
- W2090038499 cites W1987649367 @default.
- W2090038499 cites W1988744799 @default.
- W2090038499 cites W1989605738 @default.
- W2090038499 cites W1990554391 @default.
- W2090038499 cites W2000349524 @default.
- W2090038499 cites W2002017754 @default.
- W2090038499 cites W2003937760 @default.
- W2090038499 cites W2005764159 @default.
- W2090038499 cites W2009276462 @default.
- W2090038499 cites W2011892782 @default.
- W2090038499 cites W2014058353 @default.
- W2090038499 cites W2015832516 @default.
- W2090038499 cites W2015859860 @default.
- W2090038499 cites W2018422556 @default.
- W2090038499 cites W2018641577 @default.
- W2090038499 cites W2021129026 @default.
- W2090038499 cites W2022132013 @default.
- W2090038499 cites W2022374631 @default.
- W2090038499 cites W2026175012 @default.
- W2090038499 cites W2029737457 @default.
- W2090038499 cites W2031536699 @default.
- W2090038499 cites W2031881009 @default.
- W2090038499 cites W2032102101 @default.
- W2090038499 cites W2033228549 @default.
- W2090038499 cites W2043054174 @default.
- W2090038499 cites W2044290370 @default.
- W2090038499 cites W2045380458 @default.
- W2090038499 cites W2045452020 @default.
- W2090038499 cites W2054018067 @default.
- W2090038499 cites W2054854596 @default.
- W2090038499 cites W2057417107 @default.
- W2090038499 cites W2060282975 @default.
- W2090038499 cites W2062378986 @default.
- W2090038499 cites W2068119233 @default.
- W2090038499 cites W2072742323 @default.
- W2090038499 cites W2078818620 @default.
- W2090038499 cites W2078821762 @default.
- W2090038499 cites W2089139986 @default.
- W2090038499 cites W2093361250 @default.
- W2090038499 cites W2098433114 @default.
- W2090038499 cites W2100659636 @default.
- W2090038499 cites W2101765077 @default.
- W2090038499 cites W2103912593 @default.
- W2090038499 cites W2120792823 @default.
- W2090038499 cites W2122341467 @default.
- W2090038499 cites W2126008048 @default.
- W2090038499 cites W2127806759 @default.
- W2090038499 cites W2129796656 @default.
- W2090038499 cites W2134294998 @default.
- W2090038499 cites W2143505819 @default.
- W2090038499 cites W2143751403 @default.
- W2090038499 cites W2144719264 @default.
- W2090038499 cites W2146089032 @default.
- W2090038499 cites W2156774678 @default.
- W2090038499 cites W2165268250 @default.
- W2090038499 cites W2167419359 @default.
- W2090038499 cites W2312895380 @default.
- W2090038499 cites W28486177 @default.
- W2090038499 cites W3023019793 @default.
- W2090038499 cites W4252205273 @default.
- W2090038499 cites W5329134 @default.
- W2090038499 cites W61346947 @default.
- W2090038499 doi "https://doi.org/10.1038/nprot.2014.018" @default.
- W2090038499 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4063296" @default.
- W2090038499 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24457333" @default.
- W2090038499 hasPublicationYear "2014" @default.
- W2090038499 type Work @default.