Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090043370> ?p ?o ?g. }
- W2090043370 abstract "Glutathione (gamma-L-glutamyl-L-cysteinyl-glycine; GSH) shares structural similarities with the beta-lactam biosynthetic intermediate ACV-tripeptide (delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine). Not surprisingly, GSH has been reported to inhibit the beta-lactam biosynthetic machinery quite effectively and, hence, strategies to decrease the intracellular GSH concentrations without influencing negatively the physiological status of idiophasic mycelia would attract industrial interests. Here we present a detailed map of the GSH metabolic network of P. chrysogenum and show a promising way to keep the GSH pool selectively down under penicillin producing conditions. This procedure includes a well-controlled and transient lowering of pH at the beginning of the production phase, and it relies on the GSH-dependent detoxification of the protonophore penicillin side-chain precursors phenoxyacetic acid (POA) and phenylacetic acid (PA). Encouraging preliminary fed-batch fermentation experiments have been performed to test this technological proposal. Interestingly, the mechanism of the activation of POA and PA to the appropriate CoA derivatives has remained yet to be answered but the involvement of GSH seems to be rather unlikely in this case. Our data also challenge the hypothesis that the formation of different kinds of penicillins would be an alternative to GSH-dependent detoxification processes in P. chrysogenum." @default.
- W2090043370 created "2016-06-24" @default.
- W2090043370 creator A5006445023 @default.
- W2090043370 creator A5026374518 @default.
- W2090043370 creator A5034076391 @default.
- W2090043370 creator A5036150644 @default.
- W2090043370 creator A5082367364 @default.
- W2090043370 date "2001-09-01" @default.
- W2090043370 modified "2023-09-26" @default.
- W2090043370 title "The glutathione metabolism of the b-lactam producer filamentous fungusPenicillium chrysogenum" @default.
- W2090043370 cites W1011347604 @default.
- W2090043370 cites W1505266621 @default.
- W2090043370 cites W1537627033 @default.
- W2090043370 cites W1537930294 @default.
- W2090043370 cites W1572204645 @default.
- W2090043370 cites W1575831698 @default.
- W2090043370 cites W1595716614 @default.
- W2090043370 cites W189916868 @default.
- W2090043370 cites W1941201199 @default.
- W2090043370 cites W1965318717 @default.
- W2090043370 cites W1970749661 @default.
- W2090043370 cites W1971276372 @default.
- W2090043370 cites W1974315179 @default.
- W2090043370 cites W1975642625 @default.
- W2090043370 cites W1990682817 @default.
- W2090043370 cites W1994018933 @default.
- W2090043370 cites W1994664024 @default.
- W2090043370 cites W2004384215 @default.
- W2090043370 cites W2005527866 @default.
- W2090043370 cites W2009189569 @default.
- W2090043370 cites W2014003865 @default.
- W2090043370 cites W2018655453 @default.
- W2090043370 cites W2026766302 @default.
- W2090043370 cites W2031190064 @default.
- W2090043370 cites W2032281316 @default.
- W2090043370 cites W2034770144 @default.
- W2090043370 cites W2035059848 @default.
- W2090043370 cites W2038503110 @default.
- W2090043370 cites W2042503541 @default.
- W2090043370 cites W2047991483 @default.
- W2090043370 cites W2049528785 @default.
- W2090043370 cites W2052942825 @default.
- W2090043370 cites W2054796451 @default.
- W2090043370 cites W2056822295 @default.
- W2090043370 cites W2057865646 @default.
- W2090043370 cites W2065724201 @default.
- W2090043370 cites W2066293289 @default.
- W2090043370 cites W2068689370 @default.
- W2090043370 cites W2073304584 @default.
- W2090043370 cites W2075514851 @default.
- W2090043370 cites W2081663028 @default.
- W2090043370 cites W2082426921 @default.
- W2090043370 cites W2082646927 @default.
- W2090043370 cites W2085353654 @default.
- W2090043370 cites W2088944136 @default.
- W2090043370 cites W2090223746 @default.
- W2090043370 cites W2094357622 @default.
- W2090043370 cites W2100501843 @default.
- W2090043370 cites W2110234289 @default.
- W2090043370 cites W2116446055 @default.
- W2090043370 cites W2123114163 @default.
- W2090043370 cites W2123685092 @default.
- W2090043370 cites W2128171188 @default.
- W2090043370 cites W2132209773 @default.
- W2090043370 cites W2137683543 @default.
- W2090043370 cites W2138338551 @default.
- W2090043370 cites W2160448062 @default.
- W2090043370 cites W2165994558 @default.
- W2090043370 cites W2264323239 @default.
- W2090043370 cites W254825623 @default.
- W2090043370 cites W2743736005 @default.
- W2090043370 cites W278709392 @default.
- W2090043370 cites W4230976681 @default.
- W2090043370 cites W4298026545 @default.
- W2090043370 cites W758260955 @default.
- W2090043370 cites W2137564704 @default.
- W2090043370 doi "https://doi.org/10.1556/amicr.48.2001.3-4.9" @default.
- W2090043370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11791340" @default.
- W2090043370 hasPublicationYear "2001" @default.
- W2090043370 type Work @default.
- W2090043370 sameAs 2090043370 @default.
- W2090043370 citedByCount "3" @default.
- W2090043370 crossrefType "journal-article" @default.
- W2090043370 hasAuthorship W2090043370A5006445023 @default.
- W2090043370 hasAuthorship W2090043370A5026374518 @default.
- W2090043370 hasAuthorship W2090043370A5034076391 @default.
- W2090043370 hasAuthorship W2090043370A5036150644 @default.
- W2090043370 hasAuthorship W2090043370A5082367364 @default.
- W2090043370 hasBestOaLocation W20900433702 @default.
- W2090043370 hasConcept C142724271 @default.
- W2090043370 hasConcept C167392928 @default.
- W2090043370 hasConcept C174802600 @default.
- W2090043370 hasConcept C181199279 @default.
- W2090043370 hasConcept C185592680 @default.
- W2090043370 hasConcept C204787440 @default.
- W2090043370 hasConcept C2777270571 @default.
- W2090043370 hasConcept C2777573094 @default.
- W2090043370 hasConcept C2777756961 @default.
- W2090043370 hasConcept C2778990612 @default.
- W2090043370 hasConcept C2780497538 @default.