FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090054328> ?p ?o ?g. }

• W2090054328 endingPage "1660" @default.
• W2090054328 startingPage "1652" @default.
• W2090054328 abstract "The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the metaphase of the second meiotic cell division (M-II) stage to develop to the blastocyst stage after parthenogenetic activation (PA) or intracytoplasmic sperm injection (ICSI). In Experiment 1, we examined the effects of exposure time of oocytes to cryoprotectants (CPA) on their in vitro development after PA. In vitro matured (IVM) oocytes were placed in 10% dimethylsulfoxide (DMSO) + 10% ethylene glycol (EG) for 1 min and then exposed to 20% DMSO + 20% EG + 0.5 M sucrose for 30 s, 45 s or 60 s (1 min + 30 s, 1 min + 45 s and 1 min + 60 s groups, respectively). The oocytes were then exposed to warming solution (TCM199 HEPES + 20% FBS and 0.5M sucrose) for 5 min and then washed in TCM199 HEPES + 20% FBS for 5 min. IVM oocytes without CPA treatments served as a control group. The viability assessed by fluorescein diacetate (FDA) staining was 100% in all groups. The developmental rates after PA to the blastocyst stage between 1min+30s (16%) and control (26%) groups did not differ significantly, but they were significantly higher than those in 1 min + 45 s (10%) and 1 min + 60 s (2%) groups. In Experiment 2, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on the in vitro development after PA of oocytes vitrified by the microdrop method. The viabilities in vitrified 1 min + 30 s, 1 min + 45 s and the control (without CPA treatments) groups were not different (97%, 95% and 100%, respectively). The development of surviving oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (8%) was significantly higher than that in the vitrified 1 min + 45 s group (4%) and significantly lower than those in control group (26%). In Experiment 3, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on in vitro development after ICSI of vitrified oocytes. Viabilities in vitrified oocytes among 1 min + 30 s, 1 min + 45 s and control groups were not different (96%, 91% and 100%, respectively). After ICSI, vitrified-warmed oocytes were activated and oocytes with the second polar body were cultured for 7 days. The development of ICSI oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (11%) was significantly higher than that in the vitrified 1 min + 45 s (7%) group and significantly lower than those in control group (23%). In conclusion, our study demonstrated that the 1 min + 30 s CPA treatment regimen could yield the highest blastocyst formation rates after PA and ICSI for oocytes vitrified by the microdrop method." @default.
• W2090054328 created "2016-06-24" @default.
• W2090054328 creator A5008860633 @default.
• W2090054328 creator A5009980087 @default.
• W2090054328 creator A5019934818 @default.
• W2090054328 creator A5020870072 @default.
• W2090054328 creator A5069697619 @default.
• W2090054328 date "2011-06-01" @default.
• W2090054328 modified "2023-10-16" @default.
• W2090054328 title "In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection" @default.
• W2090054328 cites W1545776712 @default.
• W2090054328 cites W1601444578 @default.
• W2090054328 cites W1829905667 @default.
• W2090054328 cites W1965413255 @default.
• W2090054328 cites W1974515898 @default.
• W2090054328 cites W1976806300 @default.
• W2090054328 cites W1978413384 @default.
• W2090054328 cites W1978525874 @default.
• W2090054328 cites W1979676807 @default.
• W2090054328 cites W1987244442 @default.
• W2090054328 cites W1987720030 @default.
• W2090054328 cites W1992792379 @default.
• W2090054328 cites W1997015748 @default.
• W2090054328 cites W2006813962 @default.
• W2090054328 cites W2010098556 @default.
• W2090054328 cites W2012940952 @default.
• W2090054328 cites W2016255277 @default.
• W2090054328 cites W2017798219 @default.
• W2090054328 cites W2042193321 @default.
• W2090054328 cites W2043952154 @default.
• W2090054328 cites W2055624916 @default.
• W2090054328 cites W2073017414 @default.
• W2090054328 cites W2076923979 @default.
• W2090054328 cites W2079812680 @default.
• W2090054328 cites W2084392013 @default.
• W2090054328 cites W2085371593 @default.
• W2090054328 cites W2088610385 @default.
• W2090054328 cites W2100001216 @default.
• W2090054328 cites W2112392835 @default.
• W2090054328 cites W2120111723 @default.
• W2090054328 cites W2131391649 @default.
• W2090054328 cites W2139789898 @default.
• W2090054328 cites W2150250893 @default.
• W2090054328 cites W2153392593 @default.
• W2090054328 cites W2157480432 @default.
• W2090054328 cites W2168807103 @default.
• W2090054328 cites W2171556951 @default.
• W2090054328 cites W2292739086 @default.
• W2090054328 doi "https://doi.org/10.1016/j.theriogenology.2010.12.028" @default.
• W2090054328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21356549" @default.
• W2090054328 hasPublicationYear "2011" @default.
• W2090054328 type Work @default.
• W2090054328 sameAs 2090054328 @default.
• W2090054328 citedByCount "18" @default.
• W2090054328 countsByYear W20900543282012 @default.
• W2090054328 countsByYear W20900543282013 @default.
• W2090054328 countsByYear W20900543282014 @default.
• W2090054328 countsByYear W20900543282015 @default.
• W2090054328 countsByYear W20900543282016 @default.
• W2090054328 countsByYear W20900543282017 @default.
• W2090054328 countsByYear W20900543282018 @default.
• W2090054328 countsByYear W20900543282020 @default.
• W2090054328 countsByYear W20900543282022 @default.
• W2090054328 countsByYear W20900543282023 @default.
• W2090054328 crossrefType "journal-article" @default.
• W2090054328 hasAuthorship W2090054328A5008860633 @default.
• W2090054328 hasAuthorship W2090054328A5009980087 @default.
• W2090054328 hasAuthorship W2090054328A5019934818 @default.
• W2090054328 hasAuthorship W2090054328A5020870072 @default.
• W2090054328 hasAuthorship W2090054328A5069697619 @default.
• W2090054328 hasConcept C104317684 @default.
• W2090054328 hasConcept C16685009 @default.
• W2090054328 hasConcept C179933525 @default.
• W2090054328 hasConcept C185592680 @default.
• W2090054328 hasConcept C196843134 @default.
• W2090054328 hasConcept C2775966511 @default.
• W2090054328 hasConcept C2778177303 @default.
• W2090054328 hasConcept C2778278907 @default.
• W2090054328 hasConcept C2781087480 @default.
• W2090054328 hasConcept C512716672 @default.
• W2090054328 hasConcept C55493867 @default.
• W2090054328 hasConcept C68528507 @default.
• W2090054328 hasConcept C71924100 @default.
• W2090054328 hasConcept C86803240 @default.
• W2090054328 hasConcept C87073359 @default.
• W2090054328 hasConcept C95444343 @default.
• W2090054328 hasConceptScore W2090054328C104317684 @default.
• W2090054328 hasConceptScore W2090054328C16685009 @default.
• W2090054328 hasConceptScore W2090054328C179933525 @default.
• W2090054328 hasConceptScore W2090054328C185592680 @default.
• W2090054328 hasConceptScore W2090054328C196843134 @default.
• W2090054328 hasConceptScore W2090054328C2775966511 @default.
• W2090054328 hasConceptScore W2090054328C2778177303 @default.
• W2090054328 hasConceptScore W2090054328C2778278907 @default.
• W2090054328 hasConceptScore W2090054328C2781087480 @default.
• W2090054328 hasConceptScore W2090054328C512716672 @default.
• W2090054328 hasConceptScore W2090054328C55493867 @default.
• W2090054328 hasConceptScore W2090054328C68528507 @default.

• next