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- W2090069735 abstract "Recently a new human myeloma cell line (Karpas 707H) has been developed for the efficient generation of stable human hybridomas. Here we describe the first molecular characterization of human monoclonal antibodies (MAbs) produced by a human counterpart to mouse myeloma cells. We studied 30 of the hybridomas generated by fusions to tonsil lymphocytes by DNA sequencing of rearranged V-genes, and have analyzed germ-line diversity, somatic hypermutation, and heavy- and light-chain pairings. Our results suggest that the hybridoma-derived antibodies are representative of antibodies from populations of human lymphocytes and at different stages in the maturation of the response; the use of Karpas 707H myeloma for human hybridoma fusions may therefore provide a valuable tool for analysis of the human antibody responses." @default.
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- W2090069735 date "2001-10-01" @default.
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- W2090069735 title "Molecular Characterization of Human Monoclonal Antibodies Derived from Fusions of Tonsil Lymphocytes with a Human Myeloma Cell Line" @default.
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- W2090069735 doi "https://doi.org/10.1089/15368590152740680" @default.
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