FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090176421> ?p ?o ?g. }

• W2090176421 endingPage "e91535" @default.
• W2090176421 startingPage "e91535" @default.
• W2090176421 abstract "Eukaryotic polycistronic transcription units are rare and only a few examples are known, mostly being the outcome of serendipitous discovery. We claim that nonsense-mediated mRNA decay (NMD) immune structure is a common characteristic of polycistronic transcripts, and that this immunity is an emergent property derived from all functional CDSs. The human RefSeq transcriptome was computationally screened for transcripts capable of eliciting NMD, and which contain an additional ORF(s) potentially capable of rescuing the transcript from NMD. Transcripts were further analyzed implementing domain-based strategies in order to estimate the potential of the candidate ORF to encode a functional protein. Consequently, we predict the existence of forty nine novel polycistronic transcripts. Experimental verification was carried out utilizing two different types of analyses. First, five Gene Expression Omnibus (GEO) datasets from published NMD-inhibition studies were used, aiming to explore whether a given mRNA is indeed insensitive to NMD. All known bicistronic transcripts and eleven out of the twelve predicted genes that were analyzed, displayed NMD insensitivity using various NMD inhibitors. For three genes, a mixed expression pattern was observed presenting both NMD sensitivity and insensitivity in different cell types. Second, we used published global translation initiation sequencing data from HEK293 cells to verify the existence of translation initiation sites in our predicted polycistronic genes. In five of our genes, the predicted rescuing uORFs are indeed identified as translation initiation sites, and in two additional genes, one of two predicted rescuing uORF is verified. These results validate our computational analysis and reinforce the possibility that NMD-immune architecture is a parameter by which polycistronic genes can be identified. Moreover, we present evidence for NMD-mediated regulation controlling the production of one or more proteins encoded in the polycistronic transcript." @default.
• W2090176421 created "2016-06-24" @default.
• W2090176421 creator A5033903901 @default.
• W2090176421 creator A5079203294 @default.
• W2090176421 date "2014-03-12" @default.
• W2090176421 modified "2023-09-27" @default.
• W2090176421 title "Nonsense-Mediated mRNA Decay Immunity Can Help Identify Human Polycistronic Transcripts" @default.
• W2090176421 cites W1965395430 @default.
• W2090176421 cites W1969264016 @default.
• W2090176421 cites W1974670349 @default.
• W2090176421 cites W1975304302 @default.
• W2090176421 cites W1976860224 @default.
• W2090176421 cites W1997980793 @default.
• W2090176421 cites W1998334193 @default.
• W2090176421 cites W1999144429 @default.
• W2090176421 cites W2008410413 @default.
• W2090176421 cites W2010755507 @default.
• W2090176421 cites W2021006884 @default.
• W2090176421 cites W2022366078 @default.
• W2090176421 cites W2024039709 @default.
• W2090176421 cites W2024751967 @default.
• W2090176421 cites W2027195993 @default.
• W2090176421 cites W2036895652 @default.
• W2090176421 cites W2037313242 @default.
• W2090176421 cites W2041122594 @default.
• W2090176421 cites W2041322170 @default.
• W2090176421 cites W2045773985 @default.
• W2090176421 cites W2047002583 @default.
• W2090176421 cites W2049528674 @default.
• W2090176421 cites W2053535202 @default.
• W2090176421 cites W2055043387 @default.
• W2090176421 cites W2056673221 @default.
• W2090176421 cites W2061921466 @default.
• W2090176421 cites W2064036807 @default.
• W2090176421 cites W2066841033 @default.
• W2090176421 cites W2071549023 @default.
• W2090176421 cites W2072590456 @default.
• W2090176421 cites W2074313830 @default.
• W2090176421 cites W2079517684 @default.
• W2090176421 cites W2083088823 @default.
• W2090176421 cites W2084683008 @default.
• W2090176421 cites W2084715034 @default.
• W2090176421 cites W2085233756 @default.
• W2090176421 cites W2092108158 @default.
• W2090176421 cites W2092546166 @default.
• W2090176421 cites W2096824423 @default.
• W2090176421 cites W2099927467 @default.
• W2090176421 cites W2101681684 @default.
• W2090176421 cites W2105707878 @default.
• W2090176421 cites W2107903949 @default.
• W2090176421 cites W2116654909 @default.
• W2090176421 cites W2119010032 @default.
• W2090176421 cites W2121067536 @default.
• W2090176421 cites W2129663574 @default.
• W2090176421 cites W2132865063 @default.
• W2090176421 cites W2134548352 @default.
• W2090176421 cites W2137898238 @default.
• W2090176421 cites W2145070407 @default.
• W2090176421 cites W2145396128 @default.
• W2090176421 cites W2150245940 @default.
• W2090176421 cites W2152974197 @default.
• W2090176421 cites W2154128645 @default.
• W2090176421 cites W2154696127 @default.
• W2090176421 cites W2160660518 @default.
• W2090176421 cites W2168546553 @default.
• W2090176421 cites W2168847939 @default.
• W2090176421 cites W2170418856 @default.
• W2090176421 cites W4230981326 @default.
• W2090176421 cites W4237775567 @default.
• W2090176421 cites W2088686410 @default.
• W2090176421 doi "https://doi.org/10.1371/journal.pone.0091535" @default.
• W2090176421 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3951408" @default.
• W2090176421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24621851" @default.
• W2090176421 hasPublicationYear "2014" @default.
• W2090176421 type Work @default.
• W2090176421 sameAs 2090176421 @default.
• W2090176421 citedByCount "1" @default.
• W2090176421 countsByYear W20901764212018 @default.
• W2090176421 crossrefType "journal-article" @default.
• W2090176421 hasAuthorship W2090176421A5033903901 @default.
• W2090176421 hasAuthorship W2090176421A5079203294 @default.
• W2090176421 hasBestOaLocation W20901764211 @default.
• W2090176421 hasConcept C104317684 @default.
• W2090176421 hasConcept C105580179 @default.
• W2090176421 hasConcept C149364088 @default.
• W2090176421 hasConcept C150194340 @default.
• W2090176421 hasConcept C162317418 @default.
• W2090176421 hasConcept C167625842 @default.
• W2090176421 hasConcept C4718897 @default.
• W2090176421 hasConcept C47289529 @default.
• W2090176421 hasConcept C54355233 @default.
• W2090176421 hasConcept C54458228 @default.
• W2090176421 hasConcept C67705224 @default.
• W2090176421 hasConcept C70219777 @default.
• W2090176421 hasConcept C70721500 @default.
• W2090176421 hasConcept C86803240 @default.
• W2090176421 hasConceptScore W2090176421C104317684 @default.
• W2090176421 hasConceptScore W2090176421C105580179 @default.

• next