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• W2090220412 abstract "Fourth generation poly(propylene imine) dendrimer has been completely or partially functionalized with guanidinium groups. In the second case, the remaining toxic primary amino groups of the dendrimers were reacted with propylene oxide affording the corresponding hydroxylated derivatives. Five derivatives have been prepared bearing 0, 6, 12, 24 or 32 guanidinium groups. These guanidinylated dendrimers were interacted with plasmid DNA affording the corresponding dendriplexes. The complexes were physicochemically characterized by dynamic light scattering, ζ-potential measurements and AFM, while the extent of complexation was evaluated by agarose gel electrophoresis. Furthermore, their transfection efficiency was assessed employing HEK 293 and COS-7 cell lines, while the serum effect was studied in HEK 293 cells. It was found that complete replacement of primary amino groups with the hydroxylated moieties resulted in complete loss of transfection efficiency. On the contrary, guanidinylation of the parent dendrimer resulted to significant enhancement of its transfection efficiency, this enhancement being dependent on the number of guanidinium groups per dendrimer, the cell line used and the presence or absence of FBS. The fully guanidinylated dendrimer exhibited the best transfection efficiency under all the conditions studied. This efficiency has been attributed to the enhanced penetrating ability of the guanidinylated dendrimers due to the accumulation of the guanidinium group at the dendrimeric surface. It was also found that the derivative with 12 guanidinium groups exhibited the lowest toxicity. The reduction of toxicity was apparently attributed to the decrease of the external primary amino groups coupled with the presence of hydroxylated moieties located at the dendrimeric surface. The functionalization strategy employed leads to dendrimeric derivatives that combine satisfactory transfection efficiency and cytotoxicity." @default.
• W2090220412 created "2016-06-24" @default.
• W2090220412 creator A5011220101 @default.
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• W2090220412 creator A5032673653 @default.
• W2090220412 creator A5046108584 @default.
• W2090220412 date "2007-01-01" @default.
• W2090220412 modified "2023-10-17" @default.
• W2090220412 title "Synthesis and characterization of guanidinylated poly(propylene imine) dendrimers as gene transfection agents" @default.
• W2090220412 cites W1483781798 @default.
• W2090220412 cites W1493354087 @default.
• W2090220412 cites W1499573758 @default.
• W2090220412 cites W1560355462 @default.
• W2090220412 cites W1967078049 @default.
• W2090220412 cites W1968324199 @default.
• W2090220412 cites W1973458116 @default.
• W2090220412 cites W1974058125 @default.
• W2090220412 cites W1975873807 @default.
• W2090220412 cites W1976704473 @default.
• W2090220412 cites W1977103369 @default.
• W2090220412 cites W1979146520 @default.
• W2090220412 cites W1985283983 @default.
• W2090220412 cites W1992557609 @default.
• W2090220412 cites W1994354502 @default.
• W2090220412 cites W1996545689 @default.
• W2090220412 cites W1997818739 @default.
• W2090220412 cites W1998154525 @default.
• W2090220412 cites W1998534634 @default.
• W2090220412 cites W2001018320 @default.
• W2090220412 cites W2001574729 @default.
• W2090220412 cites W2002903271 @default.
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• W2090220412 cites W2009508594 @default.
• W2090220412 cites W2010655741 @default.
• W2090220412 cites W2011085652 @default.
• W2090220412 cites W2014328495 @default.
• W2090220412 cites W2018470991 @default.
• W2090220412 cites W2021755139 @default.
• W2090220412 cites W2023104469 @default.
• W2090220412 cites W2028322263 @default.
• W2090220412 cites W2028939305 @default.
• W2090220412 cites W2029346749 @default.
• W2090220412 cites W2032689379 @default.
• W2090220412 cites W2042143995 @default.
• W2090220412 cites W2056843008 @default.
• W2090220412 cites W2058067959 @default.
• W2090220412 cites W2058490945 @default.
• W2090220412 cites W2060071401 @default.
• W2090220412 cites W2062253243 @default.
• W2090220412 cites W2069789263 @default.
• W2090220412 cites W2070794824 @default.
• W2090220412 cites W2073581953 @default.
• W2090220412 cites W2075231390 @default.
• W2090220412 cites W2077850392 @default.
• W2090220412 cites W2078327940 @default.
• W2090220412 cites W2081498971 @default.
• W2090220412 cites W2083707689 @default.
• W2090220412 cites W2084121938 @default.
• W2090220412 cites W2085516892 @default.
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• W2090220412 cites W2088902418 @default.
• W2090220412 cites W2099871857 @default.
• W2090220412 cites W2108836062 @default.
• W2090220412 cites W2110303380 @default.
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• W2090220412 cites W2123097988 @default.
• W2090220412 cites W2123650819 @default.
• W2090220412 cites W2126818937 @default.
• W2090220412 cites W2127376756 @default.
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• W2090220412 cites W2148284816 @default.
• W2090220412 cites W2153444599 @default.
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• W2090220412 doi "https://doi.org/10.1016/j.jconrel.2006.10.019" @default.
• W2090220412 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17156881" @default.
• W2090220412 hasPublicationYear "2007" @default.
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