Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090250889> ?p ?o ?g. }
- W2090250889 endingPage "169" @default.
- W2090250889 startingPage "161" @default.
- W2090250889 abstract "TGF-beta (transforming growth factor-beta) plays a key role in osteoblast differentiation and bone development. While the ability of TGF-beta to inhibit the expression of osteoblast differentiation genes has been well documented, the mechanism of this inhibition is not yet completely characterized. Runx2, a transcription factor necessary for expression of osteoblast differentiation genes is a central target of inhibition by TGF-beta. In this study, we found that TGF-beta1 inhibits expression of osteoblast differentiation genes without altering expression of Runx2. Transient transfection experiments determined that TGF-beta1 inhibited osteocalcin promoter activity and this effect is mediated through Runx2. We further identified that there was no change in protein expression, cellular localization, or DNA binding affinity of Runx2 after TGF-beta1-treatment of osteoblasts, suggesting that Runx2 undergoes post-translational modifications following TGF-beta1 treatment. Co-immunoprecipitation experiments identified increased phosphorylation of Runx2 when differentiating osteoblasts were treated with TGF-beta1. Mitogen activated protein kinase (MAPK) inhibitors relieved the TGF-beta1-inhibitory effect of Runx2-mediated osteocalcin expression. Thus, our results suggest that TGF-beta1-inhibition of osteoblast differentiation is dependent on the MAPK pathway and this effect is most likely mediated by post-translational modification of Runx2 such as phosphorylation rather than other regulatory mechanisms." @default.
- W2090250889 created "2016-06-24" @default.
- W2090250889 creator A5013133466 @default.
- W2090250889 creator A5030287270 @default.
- W2090250889 creator A5048362281 @default.
- W2090250889 creator A5073093541 @default.
- W2090250889 creator A5088476790 @default.
- W2090250889 date "2009-01-01" @default.
- W2090250889 modified "2023-09-25" @default.
- W2090250889 title "Mitogen activated protein kinase-dependent inhibition of osteocalcin gene expression by transforming growth factor-β1" @default.
- W2090250889 cites W1965437277 @default.
- W2090250889 cites W1967402039 @default.
- W2090250889 cites W1985063935 @default.
- W2090250889 cites W1990022600 @default.
- W2090250889 cites W1992184028 @default.
- W2090250889 cites W1994596418 @default.
- W2090250889 cites W1997812904 @default.
- W2090250889 cites W2004241336 @default.
- W2090250889 cites W2010189280 @default.
- W2090250889 cites W2012448313 @default.
- W2090250889 cites W2025046506 @default.
- W2090250889 cites W2028497849 @default.
- W2090250889 cites W2041671903 @default.
- W2090250889 cites W2042839797 @default.
- W2090250889 cites W2043164695 @default.
- W2090250889 cites W2047757959 @default.
- W2090250889 cites W2053734323 @default.
- W2090250889 cites W2059276740 @default.
- W2090250889 cites W2070345664 @default.
- W2090250889 cites W2074645605 @default.
- W2090250889 cites W2075529268 @default.
- W2090250889 cites W2079089760 @default.
- W2090250889 cites W2082408176 @default.
- W2090250889 cites W2083319499 @default.
- W2090250889 cites W2089016492 @default.
- W2090250889 cites W2098846399 @default.
- W2090250889 cites W2102884982 @default.
- W2090250889 cites W2107423725 @default.
- W2090250889 cites W2108073709 @default.
- W2090250889 cites W2108281583 @default.
- W2090250889 cites W2110810005 @default.
- W2090250889 cites W2113442798 @default.
- W2090250889 cites W2114162483 @default.
- W2090250889 cites W2115155645 @default.
- W2090250889 cites W2117601137 @default.
- W2090250889 cites W2119824866 @default.
- W2090250889 cites W2122367477 @default.
- W2090250889 cites W2137298996 @default.
- W2090250889 cites W2147803180 @default.
- W2090250889 cites W2148583445 @default.
- W2090250889 cites W2152508822 @default.
- W2090250889 cites W2154568166 @default.
- W2090250889 cites W2154771084 @default.
- W2090250889 cites W2156920440 @default.
- W2090250889 cites W2157102677 @default.
- W2090250889 cites W2159868431 @default.
- W2090250889 cites W333698356 @default.
- W2090250889 cites W4251352218 @default.
- W2090250889 doi "https://doi.org/10.1002/jcb.21991" @default.
- W2090250889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19051235" @default.
- W2090250889 hasPublicationYear "2009" @default.
- W2090250889 type Work @default.
- W2090250889 sameAs 2090250889 @default.
- W2090250889 citedByCount "20" @default.
- W2090250889 countsByYear W20902508892012 @default.
- W2090250889 countsByYear W20902508892013 @default.
- W2090250889 countsByYear W20902508892014 @default.
- W2090250889 countsByYear W20902508892015 @default.
- W2090250889 countsByYear W20902508892016 @default.
- W2090250889 countsByYear W20902508892017 @default.
- W2090250889 countsByYear W20902508892019 @default.
- W2090250889 countsByYear W20902508892022 @default.
- W2090250889 crossrefType "journal-article" @default.
- W2090250889 hasAuthorship W2090250889A5013133466 @default.
- W2090250889 hasAuthorship W2090250889A5030287270 @default.
- W2090250889 hasAuthorship W2090250889A5048362281 @default.
- W2090250889 hasAuthorship W2090250889A5073093541 @default.
- W2090250889 hasAuthorship W2090250889A5088476790 @default.
- W2090250889 hasConcept C104317684 @default.
- W2090250889 hasConcept C118131993 @default.
- W2090250889 hasConcept C11960822 @default.
- W2090250889 hasConcept C131075544 @default.
- W2090250889 hasConcept C153911025 @default.
- W2090250889 hasConcept C160160445 @default.
- W2090250889 hasConcept C181199279 @default.
- W2090250889 hasConcept C184235292 @default.
- W2090250889 hasConcept C185592680 @default.
- W2090250889 hasConcept C202751555 @default.
- W2090250889 hasConcept C2776363554 @default.
- W2090250889 hasConcept C2778229498 @default.
- W2090250889 hasConcept C2778260815 @default.
- W2090250889 hasConcept C55493867 @default.
- W2090250889 hasConcept C57074206 @default.
- W2090250889 hasConcept C62478195 @default.
- W2090250889 hasConcept C86339819 @default.
- W2090250889 hasConcept C86803240 @default.
- W2090250889 hasConcept C95444343 @default.
- W2090250889 hasConcept C97029542 @default.