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• W2090426547 abstract "A novel hydrogel membrane composed of polyion complex gels was developed to transfer cultured cell sheets from thermoresponsive cell culture dishes. Polyion complexes have been prepared by mixing poly(N,N-dimethylacrylamide-co-2-acrylamido-2-methylpropane sulfonic acid) (P(DMAAm-co-AMPS)) as anionic water-soluble polymers and poly(N,N-dimethylacrylamide-co-2-acryloxyethyltrimethylammonium chloride) (P(DMAAm-co-AETA-Cl)) as cationic water-soluble polymers. Various polyion complexes have been prepared by changing the composition of two polyelectrolytes. Through a set of Teflon® ring device, polyion complexes have been modified on porous membranes by electron beams irradiated cross-linking and covalent grafting technique. NIH/3T3 mouse cells as demo cells have been cultured to prepare cell sheets. This cell sheets could be recovered by using the resulting polyion complex gels as cell sheet carriers from temperature-responsive cell culture surfaces and be transferred to new collagen-coated tissue culture polystyrene dishes. This new technique has potential applications not only for transplantation but for creation of three dimensional layered cell sheet tissues." @default.
• W2090426547 created "2016-06-24" @default.
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• W2090426547 date "2007-11-01" @default.
• W2090426547 modified "2023-10-07" @default.
• W2090426547 title "Novel cell sheet carriers using polyion complex gel modified membranes for tissue engineering technology for cell sheet manipulation and transplantation" @default.
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• W2090426547 doi "https://doi.org/10.1016/j.reactfunctpolym.2007.07.058" @default.
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