FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090508940> ?p ?o ?g. }

• W2090508940 endingPage "698" @default.
• W2090508940 startingPage "691" @default.
• W2090508940 abstract "To investigate the reverse mode function of Na(+)/Ca(2+) exchangers NCX1.1 and NCX1.5 expressed in CHO cells as well as their modulations by PKC and PKA.CHO-K1 cells were transfected with pcDNA3.1 (+) plasmid carrying cDNA of rat cardiac NCX1.1 and brain NCX1.5. The expression of NCX1.1 and NCX1.5 was examined using Western blot analysis. The intracellular Ca(2+) level ([Ca(2+)]i) was measured using Ca(2+) imaging. Whole-cell NCX currents were recorded using patch-clamp technique. Reverse mode NCX activity was elicited by perfusion with Na(+)-free medium. Ca(2+) paradox was induced by Ca(2+)-free EBSS medium, followed by Ca(2+)-containing solution (1.8 or 3.8 mmol/L CaCl2).The protein levels of NCX1.1 and NCX1.5 expressed in CHO cells had no significant difference. The reverse modes of NCX1.1 and NCX1.5 in CHO cells exhibited a transient increase of [Ca(2+)]i, which was followed by a Ca(2+) level plateau at higher external Ca(2+) concentrations. In contrast, the wild type CHO cells showed a steady increase of [Ca(2+)]i at higher external Ca(2+) concentrations. The PKC activator PMA (0.3-10 μmol/L) and PKA activator 8-Br-cAMP (10-100 μmol/L) significantly enhanced the reverse mode activity of NCX1.1 and NCX1.5 in CHO cells. NCX1.1 was 2.4-fold more sensitive to PKC activation than NCX1.5, whereas the sensitivity of the two NCX isoforms to PKA activation had no difference. Both PKC- and PKA-enhanced NCX reverse mode activities in CHO cells were suppressed by NCX inhibitor KB-R7943 (30 μmol/L).Both NCX1.1 and NCX1.5 are functional in regulating and maintaining stable [Ca(2+)]i in CHO cells and differentially regulated by PKA and PKC. The two NCX isoforms might be useful drug targets for heart and brain protection." @default.
• W2090508940 created "2016-06-24" @default.
• W2090508940 creator A5021257216 @default.
• W2090508940 creator A5024978375 @default.
• W2090508940 creator A5031874278 @default.
• W2090508940 creator A5036923059 @default.
• W2090508940 creator A5062547662 @default.
• W2090508940 creator A5062813030 @default.
• W2090508940 creator A5077114895 @default.
• W2090508940 date "2013-04-08" @default.
• W2090508940 modified "2023-10-16" @default.
• W2090508940 title "Functional comparison of the reverse mode of Na+/Ca2+ exchangers NCX1.1 and NCX1.5 expressed in CHO cells" @default.
• W2090508940 cites W1493817377 @default.
• W2090508940 cites W1507203601 @default.
• W2090508940 cites W1888944045 @default.
• W2090508940 cites W1919966300 @default.
• W2090508940 cites W1944016236 @default.
• W2090508940 cites W1963855444 @default.
• W2090508940 cites W1974157686 @default.
• W2090508940 cites W1989959217 @default.
• W2090508940 cites W1997677285 @default.
• W2090508940 cites W2004645185 @default.
• W2090508940 cites W2010894303 @default.
• W2090508940 cites W2014655613 @default.
• W2090508940 cites W2029555526 @default.
• W2090508940 cites W2031540061 @default.
• W2090508940 cites W2038469163 @default.
• W2090508940 cites W2042391144 @default.
• W2090508940 cites W2050785330 @default.
• W2090508940 cites W2052032402 @default.
• W2090508940 cites W2058549361 @default.
• W2090508940 cites W2059201211 @default.
• W2090508940 cites W2061164922 @default.
• W2090508940 cites W2065134936 @default.
• W2090508940 cites W2068499122 @default.
• W2090508940 cites W2068615953 @default.
• W2090508940 cites W2081926380 @default.
• W2090508940 cites W2084784129 @default.
• W2090508940 cites W2091781917 @default.
• W2090508940 cites W2101606385 @default.
• W2090508940 cites W2103879428 @default.
• W2090508940 cites W2115884335 @default.
• W2090508940 cites W2123433512 @default.
• W2090508940 cites W2128801406 @default.
• W2090508940 cites W2139584200 @default.
• W2090508940 cites W2141260882 @default.
• W2090508940 cites W2159160674 @default.
• W2090508940 cites W2321715923 @default.
• W2090508940 cites W2335815721 @default.
• W2090508940 cites W2340062477 @default.
• W2090508940 doi "https://doi.org/10.1038/aps.2013.4" @default.
• W2090508940 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4002881" @default.
• W2090508940 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23564083" @default.
• W2090508940 hasPublicationYear "2013" @default.
• W2090508940 type Work @default.
• W2090508940 sameAs 2090508940 @default.
• W2090508940 citedByCount "2" @default.
• W2090508940 countsByYear W20905089402015 @default.
• W2090508940 countsByYear W20905089402022 @default.
• W2090508940 crossrefType "journal-article" @default.
• W2090508940 hasAuthorship W2090508940A5021257216 @default.
• W2090508940 hasAuthorship W2090508940A5024978375 @default.
• W2090508940 hasAuthorship W2090508940A5031874278 @default.
• W2090508940 hasAuthorship W2090508940A5036923059 @default.
• W2090508940 hasAuthorship W2090508940A5062547662 @default.
• W2090508940 hasAuthorship W2090508940A5062813030 @default.
• W2090508940 hasAuthorship W2090508940A5077114895 @default.
• W2090508940 hasBestOaLocation W20905089402 @default.
• W2090508940 hasConcept C153911025 @default.
• W2090508940 hasConcept C178790620 @default.
• W2090508940 hasConcept C185592680 @default.
• W2090508940 hasConcept C2777735026 @default.
• W2090508940 hasConcept C519063684 @default.
• W2090508940 hasConcept C86803240 @default.
• W2090508940 hasConceptScore W2090508940C153911025 @default.
• W2090508940 hasConceptScore W2090508940C178790620 @default.
• W2090508940 hasConceptScore W2090508940C185592680 @default.
• W2090508940 hasConceptScore W2090508940C2777735026 @default.
• W2090508940 hasConceptScore W2090508940C519063684 @default.
• W2090508940 hasConceptScore W2090508940C86803240 @default.
• W2090508940 hasIssue "5" @default.
• W2090508940 hasLocation W20905089401 @default.
• W2090508940 hasLocation W20905089402 @default.
• W2090508940 hasLocation W20905089403 @default.
• W2090508940 hasLocation W20905089404 @default.
• W2090508940 hasOpenAccess W2090508940 @default.
• W2090508940 hasPrimaryLocation W20905089401 @default.
• W2090508940 hasRelatedWork W1985861853 @default.
• W2090508940 hasRelatedWork W1993616429 @default.
• W2090508940 hasRelatedWork W2057291960 @default.
• W2090508940 hasRelatedWork W2063099019 @default.
• W2090508940 hasRelatedWork W2359175313 @default.
• W2090508940 hasRelatedWork W2366988501 @default.
• W2090508940 hasRelatedWork W2370064380 @default.
• W2090508940 hasRelatedWork W2997937149 @default.
• W2090508940 hasRelatedWork W3123814123 @default.
• W2090508940 hasRelatedWork W3209944424 @default.
• W2090508940 hasVolume "34" @default.

• next