FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090607375> ?p ?o ?g. }

• W2090607375 endingPage "237" @default.
• W2090607375 startingPage "225" @default.
• W2090607375 abstract "Recombinant NR1a/NR2A(T671A) N-methyl-D-aspartate (NMDA) receptor-channels, which carry a point mutation in the putative glutamate binding site that reduces glutamate potency by around 1000-fold, have been expressed in Xenopus laevis oocytes and their single-channel properties examined using patch-clamp recording techniques. Shut time distributions of channel activity were fitted with a mixture of five exponential components. The first three components in each distribution were considered to occur within a channel activation as they exhibited little or no dependence on agonist concentration. Bursts of single-channel openings were defined by a critical gap length with a mean of 5.65 +/- 0.37 ms. Shut intervals with a duration longer than this value were considered to occur between separate bursts of channel openings. Distributions of the lengths of bursts of openings were fitted with a mixture of four exponential components. The longest two components carried the majority of the charge transfer in the channel recordings and had means of 7.71 +/- 1.1 and 37.7 +/- 4.3 ms. The overall probability of a channel being open during a burst was high (mean 0.92 +/- 0.01). Brief concentration jumps (1 ms) of 10 mM glutamate were applied to outside-out patches so that a comparison between the macroscopic current relaxation and steady-state single-channel activity evoked by glutamate could be made. The decay of such macroscopic currents was fitted with a single exponential component with a mean of 32.0 +/- 3.53 ms. The good agreement between macroscopic current decay following brief agonist exposure and the value for the slowest component of the burst length distribution suggests that the bursts of openings that we identified in steady-state recordings represent individual activations of recombinant NR1a/NR2A(T671A) NMDA receptor-channels. A new way of displaying geometric distributions is suggested, and the utility of a modified definition of the 'probability of being open within a burst' is discussed. The single-channel data that we present in this paper support further the idea that the point mutation T671A in the NR2A NMDA receptor subunit affects mainly the ability of glutamate to remain bound to these channels." @default.
• W2090607375 created "2016-06-24" @default.
• W2090607375 creator A5031818413 @default.
• W2090607375 creator A5064938007 @default.
• W2090607375 creator A5071314707 @default.
• W2090607375 creator A5081949745 @default.
• W2090607375 date "2000-09-01" @default.
• W2090607375 modified "2023-10-15" @default.
• W2090607375 title "Single‐channel analysis of an NMDA receptor possessing a mutation in the region of the glutamate binding site" @default.
• W2090607375 cites W1557041093 @default.
• W2090607375 cites W1908222510 @default.
• W2090607375 cites W1968653223 @default.
• W2090607375 cites W1976924550 @default.
• W2090607375 cites W1988407830 @default.
• W2090607375 cites W2014626166 @default.
• W2090607375 cites W2027991308 @default.
• W2090607375 cites W2028133023 @default.
• W2090607375 cites W2031812418 @default.
• W2090607375 cites W2032733145 @default.
• W2090607375 cites W2050050663 @default.
• W2090607375 cites W2058870251 @default.
• W2090607375 cites W2073158758 @default.
• W2090607375 cites W2082408068 @default.
• W2090607375 cites W2083517299 @default.
• W2090607375 cites W2084386763 @default.
• W2090607375 cites W2089131634 @default.
• W2090607375 cites W2090768472 @default.
• W2090607375 cites W2091765514 @default.
• W2090607375 cites W2091997673 @default.
• W2090607375 cites W2092981265 @default.
• W2090607375 cites W2128156437 @default.
• W2090607375 cites W2140899588 @default.
• W2090607375 cites W2148598721 @default.
• W2090607375 cites W2168311980 @default.
• W2090607375 cites W2336145557 @default.
• W2090607375 cites W2407495012 @default.
• W2090607375 cites W4232636520 @default.
• W2090607375 doi "https://doi.org/10.1111/j.1469-7793.2000.00225.x" @default.
• W2090607375 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2270066" @default.
• W2090607375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10970425" @default.
• W2090607375 hasPublicationYear "2000" @default.
• W2090607375 type Work @default.
• W2090607375 sameAs 2090607375 @default.
• W2090607375 citedByCount "30" @default.
• W2090607375 countsByYear W20906073752013 @default.
• W2090607375 countsByYear W20906073752016 @default.
• W2090607375 countsByYear W20906073752017 @default.
• W2090607375 crossrefType "journal-article" @default.
• W2090607375 hasAuthorship W2090607375A5031818413 @default.
• W2090607375 hasAuthorship W2090607375A5064938007 @default.
• W2090607375 hasAuthorship W2090607375A5071314707 @default.
• W2090607375 hasAuthorship W2090607375A5081949745 @default.
• W2090607375 hasBestOaLocation W20906073751 @default.
• W2090607375 hasConcept C121332964 @default.
• W2090607375 hasConcept C12554922 @default.
• W2090607375 hasConcept C147944092 @default.
• W2090607375 hasConcept C170493617 @default.
• W2090607375 hasConcept C185592680 @default.
• W2090607375 hasConcept C55493867 @default.
• W2090607375 hasConcept C61174792 @default.
• W2090607375 hasConcept C67018056 @default.
• W2090607375 hasConcept C86803240 @default.
• W2090607375 hasConceptScore W2090607375C121332964 @default.
• W2090607375 hasConceptScore W2090607375C12554922 @default.
• W2090607375 hasConceptScore W2090607375C147944092 @default.
• W2090607375 hasConceptScore W2090607375C170493617 @default.
• W2090607375 hasConceptScore W2090607375C185592680 @default.
• W2090607375 hasConceptScore W2090607375C55493867 @default.
• W2090607375 hasConceptScore W2090607375C61174792 @default.
• W2090607375 hasConceptScore W2090607375C67018056 @default.
• W2090607375 hasConceptScore W2090607375C86803240 @default.
• W2090607375 hasIssue "2" @default.
• W2090607375 hasLocation W20906073751 @default.
• W2090607375 hasLocation W20906073752 @default.
• W2090607375 hasLocation W20906073753 @default.
• W2090607375 hasOpenAccess W2090607375 @default.
• W2090607375 hasPrimaryLocation W20906073751 @default.
• W2090607375 hasRelatedWork W1974963742 @default.
• W2090607375 hasRelatedWork W2094568064 @default.
• W2090607375 hasRelatedWork W2247640687 @default.
• W2090607375 hasRelatedWork W2468600758 @default.
• W2090607375 hasRelatedWork W2902782467 @default.
• W2090607375 hasRelatedWork W2935759653 @default.
• W2090607375 hasRelatedWork W3105167352 @default.
• W2090607375 hasRelatedWork W54078636 @default.
• W2090607375 hasRelatedWork W1501425562 @default.
• W2090607375 hasRelatedWork W2954470139 @default.
• W2090607375 hasVolume "527" @default.
• W2090607375 isParatext "false" @default.
• W2090607375 isRetracted "false" @default.
• W2090607375 magId "2090607375" @default.
• W2090607375 workType "article" @default.