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• W2090612467 abstract "The structure of Mycobacterium tuberculosis dUTP nucleotidohydrolase (dUTPase) has been determined at 1.3 Å resolution in complex with magnesium ion and the non-hydrolyzable substrate analog, α,β-imido dUTP. dUTPase is an enzyme essential for depleting potentially toxic concentrations of dUTP in the cell. Given the importance of its biological role, it has been proposed that inhibiting M. tuberculosis dUTPase might be an effective means to treat tuberculosis infection in humans. The crystal structure presented here offers some insight into the potential for designing a specific inhibitor of the M. tuberculosis dUTPase enzyme. The structure also offers new insights into the mechanism of dUTP hydrolysis by providing an accurate representation of the enzyme–substrate complex in which both the metal ion and dUTP analog are included. The structure suggests that inclusion of a magnesium ion is important for stabilizing the position of the α-phosphorus for an in-line nucleophilic attack. In the absence of magnesium, the α-phosphate of dUTP can have either of the two positions which differ by 4.5 Å. A transiently ordered C-terminal loop further assists catalysis by shielding the general base, Asp83, from solvent thus elevating its pKa so that it might in turn activate a tightly bound water molecule for nucleophilic attack. The metal ion coordinates α, β, and γ phosphate groups with tridentate geometry identical with that observed in the crystal structure of DNA polymerase β complexed with magnesium and dNTP analog, revealing some common features in catalytic mechanism." @default.
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• W2090612467 date "2004-08-01" @default.
• W2090612467 modified "2023-09-26" @default.
• W2090612467 title "Crystal Structure of the Mycobacterium tuberculosis dUTPase: Insights into the Catalytic Mechanism" @default.
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• W2090612467 doi "https://doi.org/10.1016/j.jmb.2004.06.028" @default.
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