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• W2090660721 endingPage "532" @default.
• W2090660721 startingPage "513" @default.
• W2090660721 abstract "SoxS is the transcription activator of the SoxRS regulon. Despite being synthesized de novo in response to oxidative stress and despite the large disparity between the number of SoxS binding sites and the number of SoxS molecules per cell, SoxS-dependent promoters are rapidly activated after the onset of the stress. With the usual recruitment/post-recruitment mechanisms being unsuitable for activating gene expression under these conditions, we previously proposed that SoxS functions by “pre-recruitment”. In pre-recruitment, SoxS forms SoxS–RNA polymerase binary complexes, which use the DNA binding properties of SoxS and σ70 to distinguish SoxS-dependent promoters from housekeeping promoters and from the large number of sequence-equivalent but functionally irrelevant SoxS binding sites. With previous work in Escherichia coli having indicated that the most likely target on RNA polymerase for interaction with SoxS is the C-terminal domain of α, we investigated the interaction directly with the yeast two-hybrid system. We found that SoxS interacts with the αCTD and that SoxS positive control mutations disrupt the interaction. Moreover, single alanine substitutions of the αCTD that reduce or enhance SoxS activation in E. coli reduce or enhance the interaction between SoxS and the αCTD in yeast. Significantly, the critical amino acid residues lie in and around the DNA binding determinant of the αCTD, the first example of an activator contacting this determinant. These interactions were confirmed with an affinity immobilization assay. Lastly, we found that SoxS induction interferes with utilization of the UP element of an rRNA promoter. Thus, by functioning as a co-sigma factor that interacts with the DNA binding determinant of the αCTD, SoxS diverts RNA polymerase from UP-containing promoters to SoxS-activatable promoters." @default.
• W2090660721 created "2016-06-24" @default.
• W2090660721 creator A5036928408 @default.
• W2090660721 creator A5072066928 @default.
• W2090660721 date "2004-10-01" @default.
• W2090660721 modified "2023-09-25" @default.
• W2090660721 title "Novel Protein–Protein Interaction Between Escherichia coli SoxS and the DNA Binding Determinant of the RNA Polymerase α Subunit: SoxS Functions as a Co-sigma Factor and Redeploys RNA Polymerase from UP-element-containing Promoters to SoxS-dependent Promoters during Oxidative Stress" @default.
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• W2090660721 doi "https://doi.org/10.1016/j.jmb.2004.08.057" @default.
• W2090660721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15465042" @default.
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