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• W2090726547 endingPage "2242" @default.
• W2090726547 startingPage "2205" @default.
• W2090726547 abstract "Lipid bilayers are natural barriers of biological cells and cellular compartments. Membrane proteins integrated in biological membranes enable vital cell functions such as signal transduction and the transport of ions or small molecules. In order to determine the activity of a protein of interest at defined conditions, the membrane protein has to be integrated into artificial lipid bilayers immobilized on a surface. For the fabrication of such biosensors expertise is required in material science, surface and analytical chemistry, molecular biology and biotechnology. Specifically, techniques are needed for structuring surfaces in the micro- and nanometer scale, chemical modification and analysis, lipid bilayer formation, protein expression, purification and solubilization, and most importantly, protein integration into engineered lipid bilayers. Electrochemical and optical methods are suitable to detect membrane activity-related signals. The importance of structural knowledge to understand membrane protein function is obvious. Presently only a few structures of membrane proteins are solved at atomic resolution. Functional assays together with known structures of individual membrane proteins will contribute to a better understanding of vital biological processes occurring at biological membranes. Such assays will be utilized in the discovery of drugs, since membrane proteins are major drug targets." @default.
• W2090726547 created "2016-06-24" @default.
• W2090726547 creator A5016500790 @default.
• W2090726547 creator A5035736293 @default.
• W2090726547 date "2012-11-07" @default.
• W2090726547 modified "2023-09-29" @default.
• W2090726547 title "Challenges in the Development of Functional Assays of Membrane Proteins" @default.
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