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- W2090762224 abstract "Comparison of cDNA sequences from multiple sources predicts a genus of highly homologous but structurally distinct G protein α‐subunits, designated as G iα , that may include the α‐subunit of the functionally defined adenylate cyclase inhibitory G protein. Using specific oligonucleotide probes on Northern blots, we show that G iα−2 and G iα−3 , but not G iα−1 , are expressed in HL60 cells. Antisera raised against synthetic peptides from regions predicted to be conserved (AS6) and divergent (LE3) among G iα subtypes bind to a 40 kDa protein in Western blots of HL60 membranes. AS6 identifies an additional protein at 41 kDa. Thus, Northern blot and immunoblot results show that at least two G iα subtypes, a 40 kDa protein likely to correspond to G iα−2 and a 41 kDa protein possibly representing G iα−3 , may be expressed in a single cell type." @default.
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- W2090762224 date "1987-08-31" @default.
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- W2090762224 title "Detection of multiple forms of G<sub>iα</sub>in HL60 cells" @default.
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- W2090762224 doi "https://doi.org/10.1016/0014-5793(87)80356-3" @default.
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