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• W2090803416 abstract "Using monoclonal antibodies labeled with Eu3+ chelates, time-resolved fluoroimmunoassay (TRFIA) methods were developed for the determination of trace sulfamethazine (SMZ), sulfa-methoxazole (SMX), and sulfadiazine (SDZ) in environmental waters. Under the optimized conditions, the developed methods offered (i) low detection limits (9.8 ng/L SMZ, 6.1 ng/L SMX, and 5.4 ng/L SDZ, based on 90% inhibition) which were about 1 order of magnitude lower than that of the enzyme-linked immunosorbent assay (ELISA), (ii) high selectivity with no cross-reactivity (<0.05%) to similarly structured sulfonamides; (iii) high tolerance to variation of the sample pH (6.0−9.0) and salinity (0−100 mM), as well as the presence of humic acid (0−100 mg/L DOC) and heavy metals (0−1 mg/L concentration each of Cu2+, Cd2+, Hg2+, Pb2+, and As(V)) in the samples, and (iv) direct determination with low cost, high sample throughput, and low sample consumption (50−100 μL). The proposed TRFIA procedures were applied to determine sulfonamides in a variety of surface water and wastewater samples without sample pretreatment other than filtration. The satisfactory recoveries (64−127%) and reproducibilities (CV = 0.2−16%) achieved, as well as the good agreement with those given by liquid chromatography−tandem mass spectroscopy and ELISA methods, demonstrated the applicability of the proposed TRFIA methods for routine screening/quantification of sulfonamides in environmental waters." @default.
• W2090803416 created "2016-06-24" @default.
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• W2090803416 date "2009-12-29" @default.
• W2090803416 modified "2023-10-13" @default.
• W2090803416 title "Time-Resolved Fluoroimmunoassay as an Advantageous Approach for Highly Efficient Determination of Sulfonamides in Environmental Waters" @default.
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• W2090803416 doi "https://doi.org/10.1021/es903328b" @default.
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