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• W2090816932 abstract "Several derivatives of prothrombin have been prepared. Biothrombin may be obtained by activating prothrombin in several different ways with activators obtained from tissues. During the transformation of prothrombin to biothrombin there is very little reduction in molecular weight and most likely no change in solubility in 7% ( w v ) trichloroacetic acid solution. If there are any degradation products during the interaction, their quantity must be small. Biothrombin can be transformed to citrate thrombin by dissolving it in 25% sodium citrate solution. Prothrombin itself can also be transformed to citrate thrombin in the same manner; however, an intermediate forms which is called citrate autoprothrombin. The latter is an accelerator in the activation of prothrombin with activators obtained from tissues. It loses its accelerator property when it transforms to thrombin. Prothrombin can be converted to autoprothrombin in solutions containing a small amount of Ac-globulin, calcium ions, and purified platelet factor 3. Autoprothrombin has accelerator properties under certain conditions of prothrombin activation and is a degradation product of prothrombin. During its formation a considerable quantity of carbohydrate and nitrogeneous material become soluble in trichloroacetic acid. Its sedimentation constant is smaller than that of prothrombin. It can be transformed to citrate thrombin by dissolving it in 25% sodium citrate solution. Methods are described for obtaining concentrates of citrate thrombin, autoprothrombin, and biothrombin." @default.
• W2090816932 created "2016-06-24" @default.
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• W2090816932 date "1956-03-01" @default.
• W2090816932 modified "2023-10-14" @default.
• W2090816932 title "The preparation of prothrombin derivatives and an indication of their properties" @default.
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• W2090816932 doi "https://doi.org/10.1016/0003-9861(56)90312-5" @default.
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