FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090818200> ?p ?o ?g. }

• W2090818200 endingPage "28003" @default.
• W2090818200 startingPage "27991" @default.
• W2090818200 abstract "Increased O(2)* and NO production is a key mechanism of mitochondrial dysfunction in myocardial ischemia/reperfusion injury. A crucial segment of the mitochondrial electron transport chain is succinate ubiquinone reductase (SQR or Complex II). In SQR, oxidative impairment and deglutathionylation of the 70-kDa flavin protein occurs in the post-ischemic heart ( Chen, Y. R., Chen, C. L., Pfeiffer, D. R., and Zweier, J. L. (2007) J. Biol. Chem. 282, 32640-32654 ). To gain insights into the oxidative modification of the 70-kDa protein in the post-ischemic myocardium, we used the identified S-glutathionylated peptide ((77)AAFGLSEAGFNTACVTK(93)) of the 70-kDa protein as a chimeric epitope incorporating a promiscuous T cell epitope to generate a high titer polyclonal antibody, AbGSC90. Purified AbGSC90 showed a high binding affinity to isolated SQR. Antibodies of AbGSC90 moderately inhibited the electron transfer and superoxide generation activities of SQR. To test for protein nitration, rats were subjected to 30 min of coronary ligation followed by 24 h of reperfusion. Tissue homogenates were immunoprecipitated with AbGSC90 and probed with antibodies against 3-nitrotyrosine. Enhancement of protein tyrosine nitration was detected in the post-ischemic myocardium. Isolated SQR was subjected to in vitro protein nitration with peroxynitrite, leading to site-specific nitration at the 70-kDa polypeptide and impairment of SQR electron transfer activity. Protein nitration of SQR further impaired its protein-protein interaction with Complex III. Liquid chromatography/tandem mass spectrometry analysis indicated that Tyr-56 and Tyr-142 were involved in protein tyrosine nitration. When the isolated SQR was subjected to in vitro S-glutathionylation, oxidative modification and impairment mediated by peroxynitrite were significantly decreased, thus confirming the protective effect of S-glutathionylation from the oxidative damage of nitration." @default.
• W2090818200 created "2016-06-24" @default.
• W2090818200 creator A5008891263 @default.
• W2090818200 creator A5014433350 @default.
• W2090818200 creator A5022567195 @default.
• W2090818200 creator A5053625408 @default.
• W2090818200 creator A5054212573 @default.
• W2090818200 creator A5077796202 @default.
• W2090818200 creator A5084302554 @default.
• W2090818200 date "2008-10-01" @default.
• W2090818200 modified "2023-10-12" @default.
• W2090818200 title "Protein Tyrosine Nitration of the Flavin Subunit Is Associated with Oxidative Modification of Mitochondrial Complex II in the Post-ischemic Myocardium" @default.
• W2090818200 cites W1191980701 @default.
• W2090818200 cites W1221810244 @default.
• W2090818200 cites W1482087680 @default.
• W2090818200 cites W1549483548 @default.
• W2090818200 cites W1566636169 @default.
• W2090818200 cites W1571274258 @default.
• W2090818200 cites W1577460994 @default.
• W2090818200 cites W1728066272 @default.
• W2090818200 cites W1964933559 @default.
• W2090818200 cites W1964990390 @default.
• W2090818200 cites W1984693449 @default.
• W2090818200 cites W1990765857 @default.
• W2090818200 cites W2005466083 @default.
• W2090818200 cites W2008680254 @default.
• W2090818200 cites W2011095668 @default.
• W2090818200 cites W2023809310 @default.
• W2090818200 cites W2029067920 @default.
• W2090818200 cites W2038576287 @default.
• W2090818200 cites W2039306745 @default.
• W2090818200 cites W2041834967 @default.
• W2090818200 cites W2044414180 @default.
• W2090818200 cites W2047412274 @default.
• W2090818200 cites W2050357506 @default.
• W2090818200 cites W2050863140 @default.
• W2090818200 cites W2052030086 @default.
• W2090818200 cites W2056342461 @default.
• W2090818200 cites W2060026323 @default.
• W2090818200 cites W2060363769 @default.
• W2090818200 cites W2062094321 @default.
• W2090818200 cites W2076472217 @default.
• W2090818200 cites W2088658333 @default.
• W2090818200 cites W2093687559 @default.
• W2090818200 cites W2097124761 @default.
• W2090818200 cites W2105945127 @default.
• W2090818200 cites W2110565948 @default.
• W2090818200 cites W2126126820 @default.
• W2090818200 cites W2135171055 @default.
• W2090818200 cites W2149980494 @default.
• W2090818200 cites W2152248753 @default.
• W2090818200 cites W2154566486 @default.
• W2090818200 cites W2162713311 @default.
• W2090818200 cites W2271272206 @default.
• W2090818200 cites W2419494810 @default.
• W2090818200 cites W4244115987 @default.
• W2090818200 cites W70713144 @default.
• W2090818200 cites W7827006 @default.
• W2090818200 doi "https://doi.org/10.1074/jbc.m802691200" @default.
• W2090818200 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2562076" @default.
• W2090818200 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18682392" @default.
• W2090818200 hasPublicationYear "2008" @default.
• W2090818200 type Work @default.
• W2090818200 sameAs 2090818200 @default.
• W2090818200 citedByCount "61" @default.
• W2090818200 countsByYear W20908182002012 @default.
• W2090818200 countsByYear W20908182002013 @default.
• W2090818200 countsByYear W20908182002014 @default.
• W2090818200 countsByYear W20908182002015 @default.
• W2090818200 countsByYear W20908182002016 @default.
• W2090818200 countsByYear W20908182002017 @default.
• W2090818200 countsByYear W20908182002018 @default.
• W2090818200 countsByYear W20908182002019 @default.
• W2090818200 countsByYear W20908182002021 @default.
• W2090818200 countsByYear W20908182002022 @default.
• W2090818200 crossrefType "journal-article" @default.
• W2090818200 hasAuthorship W2090818200A5008891263 @default.
• W2090818200 hasAuthorship W2090818200A5014433350 @default.
• W2090818200 hasAuthorship W2090818200A5022567195 @default.
• W2090818200 hasAuthorship W2090818200A5053625408 @default.
• W2090818200 hasAuthorship W2090818200A5054212573 @default.
• W2090818200 hasAuthorship W2090818200A5077796202 @default.
• W2090818200 hasAuthorship W2090818200A5084302554 @default.
• W2090818200 hasBestOaLocation W20908182001 @default.
• W2090818200 hasConcept C153911025 @default.
• W2090818200 hasConcept C178790620 @default.
• W2090818200 hasConcept C181199279 @default.
• W2090818200 hasConcept C185592680 @default.
• W2090818200 hasConcept C2775856698 @default.
• W2090818200 hasConcept C2776151105 @default.
• W2090818200 hasConcept C2776165026 @default.
• W2090818200 hasConcept C2777622882 @default.
• W2090818200 hasConcept C2778784160 @default.
• W2090818200 hasConcept C2779385521 @default.
• W2090818200 hasConcept C2780795997 @default.
• W2090818200 hasConcept C2780829032 @default.
• W2090818200 hasConcept C28859421 @default.
• W2090818200 hasConcept C2911203004 @default.

• next