FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090838806> ?p ?o ?g. }

• W2090838806 endingPage "144" @default.
• W2090838806 startingPage "133" @default.
• W2090838806 abstract "What is already known about this subject • Flavonoids are largely recognized as potential inhibitors of platelet function, through nonspecific mechanisms such as antioxidant activity and/or inhibition of several enzymes and signalling proteins. • In addition, we, and few others, have shown that certain antiaggregant flavonoids may behave as specific TXA2 receptor (TP) ligands in platelets. • Whether flavonoids interact with TP isoforms in other cell types is not known, and direct evidence that flavonoid–TP interaction inhibits signalling downstream TP has not been shown. What this study adds • This study first demonstrates that certain flavonoids behave as ligands for both TP isoforms, not only in platelets, but also in human myometrium and in TP‐transfected HEK 293T cells. • Differences in the effect of certain flavonoids in platelet signalling, induced by either U46619 or thrombin, suggest that abrogation of downstream TP signalling is related to their specific blockage of the TP, rather than to a nonspecific effect on tyrosine kinases or other signalling proteins. Aims Flavonoids may affect platelet function by several mechanisms, including antagonism of TxA 2 receptors (TP). These TP are present in many tissues and modulate different signalling cascades. We explored whether flavonoids affect platelet TP signalling, and if they bind to TP expressed in other cell types. Methods Platelets were treated with flavonoids, or other selected inhibitors, and then stimulated with U46619. Similar assays were performed in aspirinized platelets activated with thrombin. Effects on calcium release were analysed by fluorometry and changes in whole protein tyrosine phosphorylation and activation of ERK 1/2 by Western blot analysis. The binding of flavonoids to TP in platelets, human myometrium and TPα‐ and TPβ‐transfected HEK 293T cells was explored using binding assays and the TP antagonist 3 H‐SQ29548. Results Apigenin, genistein, luteolin and quercetin impaired U46619‐induced calcium mobilization in a concentration‐dependent manner (IC 50 10–30 µ m ). These flavonoids caused a significant impairment of U46619‐induced platelet tyrosine phosphorylation and of ERK 1/2 activation. By contrast, in aspirin‐treated platelets all these flavonoids, except quercetin, displayed minor effects on thrombin‐induced calcium mobilization, ERK 1/2 and total tyrosine phosphorylation. Finally, apigenin, genistein and luteolin inhibited by >50% 3 H‐SQ29548 binding to different cell types. Conclusions These data further suggest that flavonoids may inhibit platelet function by binding to TP and by subsequent abrogation of downstream signalling. Binding of these compounds to TP occurs in human myometrium and in TP‐transfected HEK 293T cells and suggests that antagonism of TP might mediate the effects of flavonoids in different tissues." @default.
• W2090838806 created "2016-06-24" @default.
• W2090838806 creator A5000429090 @default.
• W2090838806 creator A5033392955 @default.
• W2090838806 creator A5047936186 @default.
• W2090838806 creator A5048504538 @default.
• W2090838806 creator A5055431028 @default.
• W2090838806 creator A5063482350 @default.
• W2090838806 creator A5087111637 @default.
• W2090838806 date "2007-04-10" @default.
• W2090838806 modified "2023-10-18" @default.
• W2090838806 title "Flavonoids inhibit the platelet TxA₂signalling pathway and antagonize TxA₂receptors (TP) in platelets and smooth muscle cells" @default.
• W2090838806 cites W1577086711 @default.
• W2090838806 cites W1600560134 @default.
• W2090838806 cites W1656644300 @default.
• W2090838806 cites W179499432 @default.
• W2090838806 cites W1802267091 @default.
• W2090838806 cites W1835823881 @default.
• W2090838806 cites W1976832225 @default.
• W2090838806 cites W1985973410 @default.
• W2090838806 cites W1989708266 @default.
• W2090838806 cites W1998167822 @default.
• W2090838806 cites W2006834114 @default.
• W2090838806 cites W2009490550 @default.
• W2090838806 cites W2009560933 @default.
• W2090838806 cites W2012354170 @default.
• W2090838806 cites W2013146564 @default.
• W2090838806 cites W2014046848 @default.
• W2090838806 cites W2016213835 @default.
• W2090838806 cites W2023298425 @default.
• W2090838806 cites W2024237851 @default.
• W2090838806 cites W2024638019 @default.
• W2090838806 cites W2031690762 @default.
• W2090838806 cites W2035423234 @default.
• W2090838806 cites W2037202252 @default.
• W2090838806 cites W2048009051 @default.
• W2090838806 cites W2052862118 @default.
• W2090838806 cites W2060895535 @default.
• W2090838806 cites W2073096766 @default.
• W2090838806 cites W2073121233 @default.
• W2090838806 cites W2078166953 @default.
• W2090838806 cites W2080776250 @default.
• W2090838806 cites W2084289450 @default.
• W2090838806 cites W2086554451 @default.
• W2090838806 cites W2087805394 @default.
• W2090838806 cites W2091788512 @default.
• W2090838806 cites W2100658366 @default.
• W2090838806 cites W2102378371 @default.
• W2090838806 cites W2123973590 @default.
• W2090838806 cites W2128039882 @default.
• W2090838806 cites W2129133439 @default.
• W2090838806 cites W2130963215 @default.
• W2090838806 cites W2136912731 @default.
• W2090838806 cites W2146706652 @default.
• W2090838806 cites W2151185028 @default.
• W2090838806 cites W2156348387 @default.
• W2090838806 cites W2157002083 @default.
• W2090838806 cites W2162685760 @default.
• W2090838806 cites W2317057958 @default.
• W2090838806 cites W2324091890 @default.
• W2090838806 cites W3193107558 @default.
• W2090838806 doi "https://doi.org/10.1111/j.1365-2125.2007.02881.x" @default.
• W2090838806 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2000638" @default.
• W2090838806 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17425630" @default.
• W2090838806 hasPublicationYear "2007" @default.
• W2090838806 type Work @default.
• W2090838806 sameAs 2090838806 @default.
• W2090838806 citedByCount "73" @default.
• W2090838806 countsByYear W20908388062012 @default.
• W2090838806 countsByYear W20908388062013 @default.
• W2090838806 countsByYear W20908388062014 @default.
• W2090838806 countsByYear W20908388062015 @default.
• W2090838806 countsByYear W20908388062016 @default.
• W2090838806 countsByYear W20908388062017 @default.
• W2090838806 countsByYear W20908388062018 @default.
• W2090838806 countsByYear W20908388062019 @default.
• W2090838806 countsByYear W20908388062020 @default.
• W2090838806 countsByYear W20908388062021 @default.
• W2090838806 countsByYear W20908388062022 @default.
• W2090838806 countsByYear W20908388062023 @default.
• W2090838806 crossrefType "journal-article" @default.
• W2090838806 hasAuthorship W2090838806A5000429090 @default.
• W2090838806 hasAuthorship W2090838806A5033392955 @default.
• W2090838806 hasAuthorship W2090838806A5047936186 @default.
• W2090838806 hasAuthorship W2090838806A5048504538 @default.
• W2090838806 hasAuthorship W2090838806A5055431028 @default.
• W2090838806 hasAuthorship W2090838806A5063482350 @default.
• W2090838806 hasAuthorship W2090838806A5087111637 @default.
• W2090838806 hasBestOaLocation W20908388061 @default.
• W2090838806 hasConcept C149577978 @default.
• W2090838806 hasConcept C170493617 @default.
• W2090838806 hasConcept C174510640 @default.
• W2090838806 hasConcept C185592680 @default.
• W2090838806 hasConcept C203014093 @default.
• W2090838806 hasConcept C2777292125 @default.
• W2090838806 hasConcept C3018697912 @default.
• W2090838806 hasConcept C55493867 @default.
• W2090838806 hasConcept C62478195 @default.

• next