Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090864621> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2090864621 endingPage "2885" @default.
- W2090864621 startingPage "2879" @default.
- W2090864621 abstract "In electrophoresis, the migration velocity is used for sizing DNA and proteins or for distinguishing molecules based on charge and hydrodynamic radius. Many protein and DNA assays relevant to disease diagnosis are based on such separations. However, standard protocols are not only slow (minutes to hours) but also insensitive (many molecules in a detectable band). We successfully demonstrated a high-throughput imaging approach that allows determination of the individual electrophoretic mobilities of many molecules at a time. Each measurement only requires a few milliseconds to complete. This opens up the possibility of screening single copies of DNA or proteins within single biological cells for disease markers without performing polymerase chain reaction or other biological amplification. The purpose is not to separate the DNA molecules but to identify each one on the basis of the measured electrophoretic mobility. We developed three different procedures to measure the individual molecular mobilities. The results correlate well with capillary electrophoresis (CE) experiments for the same samples (2-49 kb dsDNA) under identical separation conditions. The implication is that any electrophoresis protocols from slab gels to CE should be adaptable to single-molecule screening for disease diagnosis." @default.
- W2090864621 created "2016-06-24" @default.
- W2090864621 creator A5006295902 @default.
- W2090864621 creator A5061282830 @default.
- W2090864621 creator A5087366883 @default.
- W2090864621 creator A5088239865 @default.
- W2090864621 date "2000-05-31" @default.
- W2090864621 modified "2023-10-14" @default.
- W2090864621 title "High-Throughput Single-Molecule DNA Screening Based on Electrophoresis" @default.
- W2090864621 cites W1552514059 @default.
- W2090864621 cites W1973287468 @default.
- W2090864621 cites W2003859305 @default.
- W2090864621 cites W2011158184 @default.
- W2090864621 cites W2012139740 @default.
- W2090864621 cites W2012452399 @default.
- W2090864621 cites W2029184036 @default.
- W2090864621 cites W2054169712 @default.
- W2090864621 cites W2058912578 @default.
- W2090864621 cites W2066437194 @default.
- W2090864621 cites W2074174738 @default.
- W2090864621 cites W2089954489 @default.
- W2090864621 cites W2105184358 @default.
- W2090864621 cites W2145379960 @default.
- W2090864621 cites W2147153332 @default.
- W2090864621 cites W2949513544 @default.
- W2090864621 cites W4245723880 @default.
- W2090864621 cites W4246320257 @default.
- W2090864621 doi "https://doi.org/10.1021/ac991428n" @default.
- W2090864621 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10905322" @default.
- W2090864621 hasPublicationYear "2000" @default.
- W2090864621 type Work @default.
- W2090864621 sameAs 2090864621 @default.
- W2090864621 citedByCount "69" @default.
- W2090864621 countsByYear W20908646212012 @default.
- W2090864621 countsByYear W20908646212013 @default.
- W2090864621 countsByYear W20908646212015 @default.
- W2090864621 countsByYear W20908646212016 @default.
- W2090864621 countsByYear W20908646212018 @default.
- W2090864621 countsByYear W20908646212019 @default.
- W2090864621 crossrefType "journal-article" @default.
- W2090864621 hasAuthorship W2090864621A5006295902 @default.
- W2090864621 hasAuthorship W2090864621A5061282830 @default.
- W2090864621 hasAuthorship W2090864621A5087366883 @default.
- W2090864621 hasAuthorship W2090864621A5088239865 @default.
- W2090864621 hasConcept C101660505 @default.
- W2090864621 hasConcept C116084860 @default.
- W2090864621 hasConcept C178790620 @default.
- W2090864621 hasConcept C181199279 @default.
- W2090864621 hasConcept C185592680 @default.
- W2090864621 hasConcept C190911170 @default.
- W2090864621 hasConcept C23347341 @default.
- W2090864621 hasConcept C2900643 @default.
- W2090864621 hasConcept C32909587 @default.
- W2090864621 hasConcept C35859425 @default.
- W2090864621 hasConcept C40250595 @default.
- W2090864621 hasConcept C43617362 @default.
- W2090864621 hasConcept C552990157 @default.
- W2090864621 hasConcept C55493867 @default.
- W2090864621 hasConceptScore W2090864621C101660505 @default.
- W2090864621 hasConceptScore W2090864621C116084860 @default.
- W2090864621 hasConceptScore W2090864621C178790620 @default.
- W2090864621 hasConceptScore W2090864621C181199279 @default.
- W2090864621 hasConceptScore W2090864621C185592680 @default.
- W2090864621 hasConceptScore W2090864621C190911170 @default.
- W2090864621 hasConceptScore W2090864621C23347341 @default.
- W2090864621 hasConceptScore W2090864621C2900643 @default.
- W2090864621 hasConceptScore W2090864621C32909587 @default.
- W2090864621 hasConceptScore W2090864621C35859425 @default.
- W2090864621 hasConceptScore W2090864621C40250595 @default.
- W2090864621 hasConceptScore W2090864621C43617362 @default.
- W2090864621 hasConceptScore W2090864621C552990157 @default.
- W2090864621 hasConceptScore W2090864621C55493867 @default.
- W2090864621 hasIssue "13" @default.
- W2090864621 hasLocation W20908646211 @default.
- W2090864621 hasLocation W20908646212 @default.
- W2090864621 hasOpenAccess W2090864621 @default.
- W2090864621 hasPrimaryLocation W20908646211 @default.
- W2090864621 hasRelatedWork W15860266 @default.
- W2090864621 hasRelatedWork W2017776215 @default.
- W2090864621 hasRelatedWork W2053103690 @default.
- W2090864621 hasRelatedWork W2053680948 @default.
- W2090864621 hasRelatedWork W2075361777 @default.
- W2090864621 hasRelatedWork W2154781427 @default.
- W2090864621 hasRelatedWork W2291931440 @default.
- W2090864621 hasRelatedWork W2403455262 @default.
- W2090864621 hasRelatedWork W2613735661 @default.
- W2090864621 hasRelatedWork W1968838065 @default.
- W2090864621 hasVolume "72" @default.
- W2090864621 isParatext "false" @default.
- W2090864621 isRetracted "false" @default.
- W2090864621 magId "2090864621" @default.
- W2090864621 workType "article" @default.