Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090866819> ?p ?o ?g. }
- W2090866819 endingPage "e2535" @default.
- W2090866819 startingPage "e2535" @default.
- W2090866819 abstract "The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25–50 µl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex cytokine fluorescent bead-based immunoassays (Bio-Rad's Cytokine 17-plex kit; LINCO Inc's 29-plex kit; and RnD System's Fluorokine-Multi Analyte Profiling (MAP) base kit A and B). The LINCO Inc kit was found to be the most sensitive assay for measuring concentrations of multiple recombinant cytokines in samples that had been spiked with serial dilutions of the standard provided by the manufacturer, followed respectively by the RnD Fluorokine-(MAP) and Bio-Rad 17-plex kits. A positive correlation was found in the levels of IFN-γ measured in antigen stimulated whole blood culture supernatants by the LINCO Inc 29-plex, RnD Fluorokine-(MAP) and RnD system IFN-γ Quantikine ELISA kits across a panel of controls and stimulated samples. Researchers should take the limitation of such multiplexed assays into account when planning experiments and the most appropriate use for these tests may currently be as screening tools for the selection of promising markers for analysis by more sensitive techniques." @default.
- W2090866819 created "2016-06-24" @default.
- W2090866819 creator A5005010304 @default.
- W2090866819 creator A5023518529 @default.
- W2090866819 creator A5026222310 @default.
- W2090866819 creator A5037469433 @default.
- W2090866819 creator A5039592307 @default.
- W2090866819 creator A5040837382 @default.
- W2090866819 creator A5051182904 @default.
- W2090866819 creator A5054450393 @default.
- W2090866819 creator A5056231578 @default.
- W2090866819 creator A5076315776 @default.
- W2090866819 creator A5076984957 @default.
- W2090866819 date "2008-07-02" @default.
- W2090866819 modified "2023-09-26" @default.
- W2090866819 title "An Evaluation of Commercial Fluorescent Bead-Based Luminex Cytokine Assays" @default.
- W2090866819 cites W1966528088 @default.
- W2090866819 cites W1968827381 @default.
- W2090866819 cites W1975103807 @default.
- W2090866819 cites W2020355720 @default.
- W2090866819 cites W2055700729 @default.
- W2090866819 cites W2060535974 @default.
- W2090866819 cites W2063409320 @default.
- W2090866819 cites W2093740941 @default.
- W2090866819 cites W2097758384 @default.
- W2090866819 cites W2111811297 @default.
- W2090866819 cites W2114212834 @default.
- W2090866819 cites W2114550075 @default.
- W2090866819 cites W2130854972 @default.
- W2090866819 cites W2156260986 @default.
- W2090866819 cites W2171661751 @default.
- W2090866819 cites W2783369879 @default.
- W2090866819 cites W4255291146 @default.
- W2090866819 doi "https://doi.org/10.1371/journal.pone.0002535" @default.
- W2090866819 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2432042" @default.
- W2090866819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18596971" @default.
- W2090866819 hasPublicationYear "2008" @default.
- W2090866819 type Work @default.
- W2090866819 sameAs 2090866819 @default.
- W2090866819 citedByCount "145" @default.
- W2090866819 countsByYear W20908668192012 @default.
- W2090866819 countsByYear W20908668192013 @default.
- W2090866819 countsByYear W20908668192014 @default.
- W2090866819 countsByYear W20908668192015 @default.
- W2090866819 countsByYear W20908668192016 @default.
- W2090866819 countsByYear W20908668192017 @default.
- W2090866819 countsByYear W20908668192018 @default.
- W2090866819 countsByYear W20908668192019 @default.
- W2090866819 countsByYear W20908668192020 @default.
- W2090866819 countsByYear W20908668192021 @default.
- W2090866819 countsByYear W20908668192022 @default.
- W2090866819 countsByYear W20908668192023 @default.
- W2090866819 crossrefType "journal-article" @default.
- W2090866819 hasAuthorship W2090866819A5005010304 @default.
- W2090866819 hasAuthorship W2090866819A5023518529 @default.
- W2090866819 hasAuthorship W2090866819A5026222310 @default.
- W2090866819 hasAuthorship W2090866819A5037469433 @default.
- W2090866819 hasAuthorship W2090866819A5039592307 @default.
- W2090866819 hasAuthorship W2090866819A5040837382 @default.
- W2090866819 hasAuthorship W2090866819A5051182904 @default.
- W2090866819 hasAuthorship W2090866819A5054450393 @default.
- W2090866819 hasAuthorship W2090866819A5056231578 @default.
- W2090866819 hasAuthorship W2090866819A5076315776 @default.
- W2090866819 hasAuthorship W2090866819A5076984957 @default.
- W2090866819 hasBestOaLocation W20908668191 @default.
- W2090866819 hasConcept C10390740 @default.
- W2090866819 hasConcept C104317684 @default.
- W2090866819 hasConcept C109386097 @default.
- W2090866819 hasConcept C142724271 @default.
- W2090866819 hasConcept C153911025 @default.
- W2090866819 hasConcept C159654299 @default.
- W2090866819 hasConcept C159985019 @default.
- W2090866819 hasConcept C184112429 @default.
- W2090866819 hasConcept C185592680 @default.
- W2090866819 hasConcept C192562407 @default.
- W2090866819 hasConcept C203014093 @default.
- W2090866819 hasConcept C204787440 @default.
- W2090866819 hasConcept C2778690821 @default.
- W2090866819 hasConcept C2780420688 @default.
- W2090866819 hasConcept C2781188995 @default.
- W2090866819 hasConcept C40767141 @default.
- W2090866819 hasConcept C43617362 @default.
- W2090866819 hasConcept C55493867 @default.
- W2090866819 hasConcept C60644358 @default.
- W2090866819 hasConcept C70721500 @default.
- W2090866819 hasConcept C71924100 @default.
- W2090866819 hasConcept C86803240 @default.
- W2090866819 hasConceptScore W2090866819C10390740 @default.
- W2090866819 hasConceptScore W2090866819C104317684 @default.
- W2090866819 hasConceptScore W2090866819C109386097 @default.
- W2090866819 hasConceptScore W2090866819C142724271 @default.
- W2090866819 hasConceptScore W2090866819C153911025 @default.
- W2090866819 hasConceptScore W2090866819C159654299 @default.
- W2090866819 hasConceptScore W2090866819C159985019 @default.
- W2090866819 hasConceptScore W2090866819C184112429 @default.
- W2090866819 hasConceptScore W2090866819C185592680 @default.
- W2090866819 hasConceptScore W2090866819C192562407 @default.
- W2090866819 hasConceptScore W2090866819C203014093 @default.