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• W2090880113 startingPage "4120" @default.
• W2090880113 abstract "We report a novel modification of silicone elastomer polydimethylsiloxane (PDMS) with a polymer graft that allows interfacial bonding between an elastomer and glass substrate to be performed without exposure of the substrate to harsh treatment conditions, such as oxygen plasma. Organic molecules can thus be patterned within microfluidic channels and still remain functional post-bonding. In addition, after polymer grafting the PDMS can be stored in a desiccator for at least 40 days, and activated upon exposure to acidic buffer for bonding. The bonded devices remain fully bonded in excess of 80 psi driving pressure, with no signs of compromise to the bond integrity. Finally, we demonstrate the compatibility of our method with biological molecules using a proof-of-concept DNA sensing device, in which fluorescently-labelled DNA targets are successfully captured by a patterned probe in a device sealed using our method, while the pattern on a plasma-treated device was completely destroyed. Therefore, this method provides a much-needed alternative bonding process for incorporation of biological molecules in microfluidic devices." @default.
• W2090880113 created "2016-06-24" @default.
• W2090880113 creator A5004009491 @default.
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• W2090880113 date "2012-01-01" @default.
• W2090880113 modified "2023-10-10" @default.
• W2090880113 title "PDMS–glass bonding using grafted polymeric adhesive – alternative process flow for compatibility with patterned biological molecules" @default.
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• W2090880113 doi "https://doi.org/10.1039/c2lc40315c" @default.
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