FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090882011> ?p ?o ?g. }

• W2090882011 endingPage "224" @default.
• W2090882011 startingPage "217" @default.
• W2090882011 abstract "Biochemical data indicate μ-opioid receptor (MOR) coupling predominantly to the Gi and Go family. Additionally, MOR coupling to Gs is suggested by pharmacological assessments that have revealed excitatory MOR effects, which are resistant to pertussis toxin and sensitive to cholera toxin. However, biochemical evidence for such interactions remains elusive; Gsα has not been shown to be present in immunoprecipitate obtained using anti-MOR antibodies. In the current study, the presence of MOR in immunoprecipitate obtained with anti-Gsα antibodies was investigated using Chinese hamster ovary cells stably transfected with MOR (MOR-CHO). MOR Western analyses of opioid naïve MOR-CHO membranes immunoprecipitated using anti-Gsα antibodies reveal the presence of an ≈75–80 kDa MOR species. Interestingly, acute and chronic morphine treatment markedly enhances the magnitude of MOR that co-immunoprecipitates with Gsα, despite the concomitant down-regulation of membrane MOR protein. Enhanced co-precipitation of MOR with Gsα occurs without a concomitant increase in the immunoprecipitated Gsα protein indicating their increased association. In contrast, chronic morphine diminishes the co-immunoprecipitation of MOR with Giα. Moreover, although only a single MOR species co-immunoprecipitated with Gsα, MOR Western analysis of MOR-CHO membranes as well as immunoprecipitate obtained with either anti-MOR or anti-Giα antibodies reveals the presence of multiple molecular mass species of MOR. These data reveal the existence of a subset of MORs whose association with Gsα can be enhanced by morphine exposure. Notably, the regulation by chronic morphine of MOR association with Gsα and Giα is reciprocal. The relevance of MOR–Gsα coupling to opioid tolerance formation is discussed." @default.
• W2090882011 created "2016-06-24" @default.
• W2090882011 creator A5016263902 @default.
• W2090882011 creator A5017055008 @default.
• W2090882011 creator A5082607533 @default.
• W2090882011 date "2005-04-01" @default.
• W2090882011 modified "2023-10-16" @default.
• W2090882011 title "Biochemical demonstration of mu-opioid receptor association with Gsα: enhancement following morphine exposure" @default.
• W2090882011 cites W1497040357 @default.
• W2090882011 cites W1570939625 @default.
• W2090882011 cites W1585650753 @default.
• W2090882011 cites W1591789321 @default.
• W2090882011 cites W1600136508 @default.
• W2090882011 cites W1604997335 @default.
• W2090882011 cites W1974356365 @default.
• W2090882011 cites W1994431040 @default.
• W2090882011 cites W1996810717 @default.
• W2090882011 cites W1997266436 @default.
• W2090882011 cites W1998045779 @default.
• W2090882011 cites W2009899135 @default.
• W2090882011 cites W2011973680 @default.
• W2090882011 cites W2018464969 @default.
• W2090882011 cites W2018938470 @default.
• W2090882011 cites W2020676779 @default.
• W2090882011 cites W2026060136 @default.
• W2090882011 cites W2027197361 @default.
• W2090882011 cites W2035905569 @default.
• W2090882011 cites W2038401950 @default.
• W2090882011 cites W2058724065 @default.
• W2090882011 cites W2064731163 @default.
• W2090882011 cites W2069066733 @default.
• W2090882011 cites W2070122985 @default.
• W2090882011 cites W2071846673 @default.
• W2090882011 cites W2080182715 @default.
• W2090882011 cites W2114024266 @default.
• W2090882011 cites W2114707776 @default.
• W2090882011 cites W2115303765 @default.
• W2090882011 cites W2125850670 @default.
• W2090882011 cites W2190031288 @default.
• W2090882011 cites W2326647992 @default.
• W2090882011 cites W4293247451 @default.
• W2090882011 doi "https://doi.org/10.1016/j.molbrainres.2004.12.016" @default.
• W2090882011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15857684" @default.
• W2090882011 hasPublicationYear "2005" @default.
• W2090882011 type Work @default.
• W2090882011 sameAs 2090882011 @default.
• W2090882011 citedByCount "77" @default.
• W2090882011 countsByYear W20908820112012 @default.
• W2090882011 countsByYear W20908820112013 @default.
• W2090882011 countsByYear W20908820112014 @default.
• W2090882011 countsByYear W20908820112015 @default.
• W2090882011 countsByYear W20908820112016 @default.
• W2090882011 countsByYear W20908820112018 @default.
• W2090882011 countsByYear W20908820112019 @default.
• W2090882011 countsByYear W20908820112020 @default.
• W2090882011 countsByYear W20908820112022 @default.
• W2090882011 countsByYear W20908820112023 @default.
• W2090882011 crossrefType "journal-article" @default.
• W2090882011 hasAuthorship W2090882011A5016263902 @default.
• W2090882011 hasAuthorship W2090882011A5017055008 @default.
• W2090882011 hasAuthorship W2090882011A5082607533 @default.
• W2090882011 hasConcept C126322002 @default.
• W2090882011 hasConcept C134018914 @default.
• W2090882011 hasConcept C14397066 @default.
• W2090882011 hasConcept C153911025 @default.
• W2090882011 hasConcept C159654299 @default.
• W2090882011 hasConcept C170493617 @default.
• W2090882011 hasConcept C175656101 @default.
• W2090882011 hasConcept C203014093 @default.
• W2090882011 hasConcept C2781063702 @default.
• W2090882011 hasConcept C49773422 @default.
• W2090882011 hasConcept C55493867 @default.
• W2090882011 hasConcept C71829478 @default.
• W2090882011 hasConcept C71924100 @default.
• W2090882011 hasConcept C80631254 @default.
• W2090882011 hasConcept C86803240 @default.
• W2090882011 hasConceptScore W2090882011C126322002 @default.
• W2090882011 hasConceptScore W2090882011C134018914 @default.
• W2090882011 hasConceptScore W2090882011C14397066 @default.
• W2090882011 hasConceptScore W2090882011C153911025 @default.
• W2090882011 hasConceptScore W2090882011C159654299 @default.
• W2090882011 hasConceptScore W2090882011C170493617 @default.
• W2090882011 hasConceptScore W2090882011C175656101 @default.
• W2090882011 hasConceptScore W2090882011C203014093 @default.
• W2090882011 hasConceptScore W2090882011C2781063702 @default.
• W2090882011 hasConceptScore W2090882011C49773422 @default.
• W2090882011 hasConceptScore W2090882011C55493867 @default.
• W2090882011 hasConceptScore W2090882011C71829478 @default.
• W2090882011 hasConceptScore W2090882011C71924100 @default.
• W2090882011 hasConceptScore W2090882011C80631254 @default.
• W2090882011 hasConceptScore W2090882011C86803240 @default.
• W2090882011 hasIssue "1-2" @default.
• W2090882011 hasLocation W20908820111 @default.
• W2090882011 hasLocation W20908820112 @default.
• W2090882011 hasOpenAccess W2090882011 @default.
• W2090882011 hasPrimaryLocation W20908820111 @default.
• W2090882011 hasRelatedWork W1544947217 @default.
• W2090882011 hasRelatedWork W1974850896 @default.

• next