Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090899260> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2090899260 endingPage "60" @default.
- W2090899260 startingPage "53" @default.
- W2090899260 abstract "To investigate the variability in test results obtained with the SOS chromotest (Escherichia coli PQ37 genotoxicity assay) when varying the composition of the exogenous metabolizing system (S9 mix), we examined the influence of different S9 and NADP concentrations, of buffer pH value, of SDS concentrations, the effects of E. coli PQ37 density and centrifugation steps on the expression of beta-galactosidase (beta g) and alkaline phosphatase (ap) activity, the calculated induction factors (IFs) and SOS-inducing potencies (SOSIPs). Additionally we examined the metabolic potency (stability) of S9 mix when stored at 37 degrees C before use. Initially, we used 0-5000 ng (= 0-20 nmole) benzo[a]pyrene (B[a]P) as a reference compound for the test procedure in the presence of standard S9 mix. Subsequently, to evaluate the results of S9 mix variations we examined several polycyclic aromatic hydrocarbons (PAHs) using both the standard and a modified S9 mix composition and test protocol. We observed the highest beta g and ap activities and/or IFs using only 11-27 microliters 9000 x g liver supernatant (S9) from Aroclor 1254-induced rats per assay (20-50% of standard amount) and calibrating the S9 mix Tris buffer to pH 7.8-8.0. 60-300 micrograms NADP/assay (10-50% of standard) was sufficient for optimum activation of PAHs. In contrast to previous investigations about the variability of the SOS chromotest in the absence of a metabolizing system, higher induction factors were obtained when using higher bacterial densities (12-18 x 10(6) cfu/assay). Centrifugation steps as recommended by other investigators were not necessary when using optimum S9 amounts. The metabolic activity of S9 mix remained nearly constant approximately 20 min after preparation, but decreased to 80% of its activity in about 1 h." @default.
- W2090899260 created "2016-06-24" @default.
- W2090899260 creator A5001320477 @default.
- W2090899260 creator A5006589488 @default.
- W2090899260 creator A5008281163 @default.
- W2090899260 creator A5014991111 @default.
- W2090899260 date "1993-02-01" @default.
- W2090899260 modified "2023-10-10" @default.
- W2090899260 title "Influence of S9 mix composition on the SOS response in Escherichia coli PQ37 by polycyclic aromatic hydrocarbons" @default.
- W2090899260 cites W1964921209 @default.
- W2090899260 cites W1966333484 @default.
- W2090899260 cites W1975222291 @default.
- W2090899260 cites W2004753844 @default.
- W2090899260 cites W2016671326 @default.
- W2090899260 cites W2033213186 @default.
- W2090899260 cites W2053755832 @default.
- W2090899260 cites W2066721378 @default.
- W2090899260 cites W2071746915 @default.
- W2090899260 cites W2117856769 @default.
- W2090899260 doi "https://doi.org/10.1016/0165-1161(93)90017-t" @default.
- W2090899260 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7678915" @default.
- W2090899260 hasPublicationYear "1993" @default.
- W2090899260 type Work @default.
- W2090899260 sameAs 2090899260 @default.
- W2090899260 citedByCount "18" @default.
- W2090899260 countsByYear W20908992602019 @default.
- W2090899260 crossrefType "journal-article" @default.
- W2090899260 hasAuthorship W2090899260A5001320477 @default.
- W2090899260 hasAuthorship W2090899260A5006589488 @default.
- W2090899260 hasAuthorship W2090899260A5008281163 @default.
- W2090899260 hasAuthorship W2090899260A5014991111 @default.
- W2090899260 hasConcept C104317684 @default.
- W2090899260 hasConcept C138885662 @default.
- W2090899260 hasConcept C178790620 @default.
- W2090899260 hasConcept C185592680 @default.
- W2090899260 hasConcept C202751555 @default.
- W2090899260 hasConcept C2776678776 @default.
- W2090899260 hasConcept C2778951431 @default.
- W2090899260 hasConcept C2778959862 @default.
- W2090899260 hasConcept C2779357283 @default.
- W2090899260 hasConcept C29730261 @default.
- W2090899260 hasConcept C40231798 @default.
- W2090899260 hasConcept C41895202 @default.
- W2090899260 hasConcept C43617362 @default.
- W2090899260 hasConcept C547475151 @default.
- W2090899260 hasConcept C55493867 @default.
- W2090899260 hasConcept C7623868 @default.
- W2090899260 hasConcept C87644729 @default.
- W2090899260 hasConceptScore W2090899260C104317684 @default.
- W2090899260 hasConceptScore W2090899260C138885662 @default.
- W2090899260 hasConceptScore W2090899260C178790620 @default.
- W2090899260 hasConceptScore W2090899260C185592680 @default.
- W2090899260 hasConceptScore W2090899260C202751555 @default.
- W2090899260 hasConceptScore W2090899260C2776678776 @default.
- W2090899260 hasConceptScore W2090899260C2778951431 @default.
- W2090899260 hasConceptScore W2090899260C2778959862 @default.
- W2090899260 hasConceptScore W2090899260C2779357283 @default.
- W2090899260 hasConceptScore W2090899260C29730261 @default.
- W2090899260 hasConceptScore W2090899260C40231798 @default.
- W2090899260 hasConceptScore W2090899260C41895202 @default.
- W2090899260 hasConceptScore W2090899260C43617362 @default.
- W2090899260 hasConceptScore W2090899260C547475151 @default.
- W2090899260 hasConceptScore W2090899260C55493867 @default.
- W2090899260 hasConceptScore W2090899260C7623868 @default.
- W2090899260 hasConceptScore W2090899260C87644729 @default.
- W2090899260 hasIssue "1" @default.
- W2090899260 hasLocation W20908992601 @default.
- W2090899260 hasLocation W20908992602 @default.
- W2090899260 hasOpenAccess W2090899260 @default.
- W2090899260 hasPrimaryLocation W20908992601 @default.
- W2090899260 hasRelatedWork W1998989679 @default.
- W2090899260 hasRelatedWork W2021127632 @default.
- W2090899260 hasRelatedWork W2064126681 @default.
- W2090899260 hasRelatedWork W2088841998 @default.
- W2090899260 hasRelatedWork W2133260150 @default.
- W2090899260 hasRelatedWork W2333410658 @default.
- W2090899260 hasRelatedWork W2693666513 @default.
- W2090899260 hasRelatedWork W2726115972 @default.
- W2090899260 hasRelatedWork W3094734545 @default.
- W2090899260 hasRelatedWork W4319657267 @default.
- W2090899260 hasVolume "291" @default.
- W2090899260 isParatext "false" @default.
- W2090899260 isRetracted "false" @default.
- W2090899260 magId "2090899260" @default.
- W2090899260 workType "article" @default.