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- W2090938009 abstract "The cAMP-containing phosphoform of the regulatory subunit (RII) of type II cAMP-dependent protein kinase from rat liver has been reported to have intrinsic DNA topoisomerase I activity. We found that highly purified RII preparations from eight different sources, including rat liver, contained no detectable topoisomerase I activity. Topoisomerase I exhibited an overlapping peak of activity with RII when rat liver extracts were fractionated by diethylaminoethyl-cellulose chromatography. Topoisomerase I activity was separated from RII by subsequent cAMP affinity chromatography. The results indicate that the regulatory subunit of cAMP-dependent protein kinase does not contain intrinsic topoisomerase I activity." @default.
- W2090938009 created "2016-06-24" @default.
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- W2090938009 date "1988-04-01" @default.
- W2090938009 modified "2023-09-24" @default.
- W2090938009 title "Separation of Topoisomerase I Activity from the Regulatory Subunit of Type II CyclicAdenosine Monophosphate-Dependent Protein Kinase" @default.
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- W2090938009 doi "https://doi.org/10.1210/mend-2-4-324" @default.
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