FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090958913> ?p ?o ?g. }

• W2090958913 endingPage "130" @default.
• W2090958913 startingPage "119" @default.
• W2090958913 abstract "Modification of carboxyl groups of the (Na+ + K+)-ATPase with the water soluble 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide or of amino groups, histidine or alpha-amino groups, with diethylpyrocarbonate gives a pronounced decrease in apparent affinity for K+ for transition from the Na+-form to the K+-form at a given Na+ concentration. The fluorescence of eosin is used to monitor the conformational transitions. The decrease in apparent affinity for K+ is due to an increase in the rate of the conformational transition from E2 with K+ occluded, E2(Km+), to E2 with K+ non-occluded, E2Km+, and to a lesser degree to a decrease in the rate of the reverse reaction, while there is little or no effect on the transition between E2 in the absence of K+ and E1Nan+. The conformational transition between E2(Km+) and E2Km+ is thus much more sensitive to a change in the tertiary and/or quaternary structure than the conformational transition between E2 and E1Nan+. The pK values for the carboxyl groups are lower with the enzyme in the K+-form than in the Na+-form; in the Na+-form it seems to be lower than the lowest tested, pH 5.5. The diethylpyrocarbonate reactive amino group has a higher pK with the enzyme in the K+-form, about 8.0, than in the Na+-form, about 7.5. Plots of log K0.5 for K+ for reversal of the effect of Na+ on the conformation vs. log[Na+] at a given pH, and vs. pH (from 6.8 to 8.0) at a given Na+ concentration give straight lines, and the slope suggests that 4 Na+ on E1, E1Na4+, is replaced by 2 K+ + 1 H+ on E2, E2(K2+)H+. Modification of the amino groups with diethylpyrocarbonate shifts the log K+ vs. pH plot towards a higher K+ value at a given pH, but the slope is as for control enzyme, while the log K+ vs. log[Na+] plot becomes non-linear. None of the plots are linear after modification of the carboxyl groups with the carbodiimide." @default.
• W2090958913 created "2016-06-24" @default.
• W2090958913 creator A5057293021 @default.
• W2090958913 date "1985-09-01" @default.
• W2090958913 modified "2023-09-26" @default.
• W2090958913 title "Modification of the (Na+ + K+)-ATPase with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and with diethylpyrocarbonate. Effect on the conformational transition" @default.
• W2090958913 cites W1480115832 @default.
• W2090958913 cites W1525476801 @default.
• W2090958913 cites W1576127641 @default.
• W2090958913 cites W1587174601 @default.
• W2090958913 cites W1675484369 @default.
• W2090958913 cites W1775749144 @default.
• W2090958913 cites W1849732852 @default.
• W2090958913 cites W1967821555 @default.
• W2090958913 cites W1992629234 @default.
• W2090958913 cites W2007728507 @default.
• W2090958913 cites W2014125487 @default.
• W2090958913 cites W2019471978 @default.
• W2090958913 cites W2021463882 @default.
• W2090958913 cites W2036195707 @default.
• W2090958913 cites W2053936973 @default.
• W2090958913 cites W2084530235 @default.
• W2090958913 cites W2089056998 @default.
• W2090958913 cites W2091304906 @default.
• W2090958913 cites W2413845731 @default.
• W2090958913 cites W955725593 @default.
• W2090958913 doi "https://doi.org/10.1016/0005-2736(85)90202-0" @default.
• W2090958913 hasPublicationYear "1985" @default.
• W2090958913 type Work @default.
• W2090958913 sameAs 2090958913 @default.
• W2090958913 citedByCount "8" @default.
• W2090958913 crossrefType "journal-article" @default.
• W2090958913 hasAuthorship W2090958913A5057293021 @default.
• W2090958913 hasConcept C112887158 @default.
• W2090958913 hasConcept C155647269 @default.
• W2090958913 hasConcept C178790620 @default.
• W2090958913 hasConcept C181199279 @default.
• W2090958913 hasConcept C185592680 @default.
• W2090958913 hasConcept C2775997276 @default.
• W2090958913 hasConcept C2777339483 @default.
• W2090958913 hasConcept C2778460671 @default.
• W2090958913 hasConcept C32909587 @default.
• W2090958913 hasConcept C55493867 @default.
• W2090958913 hasConcept C71240020 @default.
• W2090958913 hasConcept C8010536 @default.
• W2090958913 hasConceptScore W2090958913C112887158 @default.
• W2090958913 hasConceptScore W2090958913C155647269 @default.
• W2090958913 hasConceptScore W2090958913C178790620 @default.
• W2090958913 hasConceptScore W2090958913C181199279 @default.
• W2090958913 hasConceptScore W2090958913C185592680 @default.
• W2090958913 hasConceptScore W2090958913C2775997276 @default.
• W2090958913 hasConceptScore W2090958913C2777339483 @default.
• W2090958913 hasConceptScore W2090958913C2778460671 @default.
• W2090958913 hasConceptScore W2090958913C32909587 @default.
• W2090958913 hasConceptScore W2090958913C55493867 @default.
• W2090958913 hasConceptScore W2090958913C71240020 @default.
• W2090958913 hasConceptScore W2090958913C8010536 @default.
• W2090958913 hasIssue "1" @default.
• W2090958913 hasLocation W20909589131 @default.
• W2090958913 hasOpenAccess W2090958913 @default.
• W2090958913 hasPrimaryLocation W20909589131 @default.
• W2090958913 hasRelatedWork W1966361305 @default.
• W2090958913 hasRelatedWork W2007545289 @default.
• W2090958913 hasRelatedWork W2048515283 @default.
• W2090958913 hasRelatedWork W2063212738 @default.
• W2090958913 hasRelatedWork W2090958913 @default.
• W2090958913 hasRelatedWork W2095161116 @default.
• W2090958913 hasRelatedWork W2329438784 @default.
• W2090958913 hasRelatedWork W2605421943 @default.
• W2090958913 hasRelatedWork W2606081567 @default.
• W2090958913 hasRelatedWork W2606340465 @default.
• W2090958913 hasVolume "819" @default.
• W2090958913 isParatext "false" @default.
• W2090958913 isRetracted "false" @default.
• W2090958913 magId "2090958913" @default.
• W2090958913 workType "article" @default.