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• W2090971215 abstract "Summary Human haematopoietic progenitor/stem cells (HPCs) differentiate into functional T cells in the thymus through a series of checkpoints. A convenient in vitro system will greatly facilitate the understanding of T‐cell development and future engineering of therapeutic T cells. In this report, we established a lentiviral vector‐engineered stromal cell line (LSC) expressing the key lymphopoiesis regulator Notch ligand, Delta‐like 1 (DL1), as feeder cells (LSC‐mDL1) supplemented with Flt3 ligand (fms‐like tyrosine kinase 3, Flt3L or FL) and interleukin‐7 for the development of T cells from CD34 + HPCs. We demonstrated T‐cell development from human HPCs with various origins including fetal thymus (FT), fetal liver (FL), cord blood (CB) and adult bone marrow (BM). The CD34 + HPCs from FT, FL and adult BM expanded more than 100‐fold before reaching the β‐selection and CD4/CD8 double‐positive T‐cell stage. The CB HPCs, on the other hand, expanded more than 1000‐fold before β‐selection. Furthermore, the time required to reach β‐selection differed for the various HPCs, 7 days for FT, 14 days for FL and CB, and 35 days for adult BM. Nevertheless, all of the T cells developed in vitro were stalled at the double‐positive or immature single‐positive stage with the exception that some CB‐derived T cells arrived at a positive selection stage. Consequently, the LSC‐mDL1 culture system illustrated diverse T‐cell development potentials of pre‐ and post‐natal and adult human BM HPCs. However, further modification of this in vitro T‐cell development system is necessary to attain fully functional T cells." @default.
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• W2090971215 date "2009-08-04" @default.
• W2090971215 modified "2023-10-16" @default.
• W2090971215 title "Diverse T-cell differentiation potentials of human fetal thymus, fetal liver, cord blood and adult bone marrow CD34 cells on lentiviral Delta-like-1-modified mouse stromal cells" @default.
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• W2090971215 doi "https://doi.org/10.1111/j.1365-2567.2008.03013.x" @default.
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