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- W2090972692 abstract "Nα-Lauryl-O-(β-d-xylopyranosyl)-l-serinamide (Xyl-Ser-C12) was synthesized as a saccharide primer to obtain oligosaccharides of glycosaminoglycan using the glycan biosynthetic potential of mouse osteosarcoma FBJ-S1 cells and Chinese hamster ovary (CHO) cells. The glycosylated products secreted into the culture medium were collected and analyzed by liquid chromatography–mass spectrometry and glycosidase digestion. The structure of the Xyl-Ser-C12 derivatives was investigated. Several glycosaminoglycan-type oligosaccharides, such as GalNAc-(GlcA-GlcNAc)n-GlcA-Gal-Gal-Xyl-Ser-C12, were detected, and identified as intermediates of the biosynthesis of heparan sulfate glycosaminoglycans. Xyl-Ser-C12 exhibited greater acceptor activity for the glycosylation of glycosaminoglycan-type oligosaccharides than p-nitrophenyl-β-d-xylopyranoside." @default.
- W2090972692 created "2016-06-24" @default.
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- W2090972692 date "2012-11-01" @default.
- W2090972692 modified "2023-09-27" @default.
- W2090972692 title "Glycosylation of N-lauryl-O-(β-d-xylopyranosyl)-l-serinamide as a saccharide primer in cells" @default.
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- W2090972692 doi "https://doi.org/10.1016/j.carres.2012.08.003" @default.
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