Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090982662> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2090982662 endingPage "141" @default.
- W2090982662 startingPage "129" @default.
- W2090982662 abstract "Micropreparative purification of the four subunits of phosphorylase kinase (molecular weights 16,680, 43,000, 113,000 and 132,000) by reversed-phase high-performance liquid chromatography has provided quantities sufficient for some of the first structural studies of these proteins. The best yield from a 25 × 0.46 cm I.D. column was obtained on a packing material (5μm C 18 ) bonded under conditions resulting in a relatively low ligand density; of a total 250 μg of protein applied, 76% was recovered. Low recoveries from 5–30-μg sample loads suggest partial irreversible adsorption. Retention of protein by the column after an initial micropreparative separation adversely affects resolution and recovery on subsequent separations. Incorporation of gradient wash steps between sample injections was necessary to maintain column performance and to prolong usable column lifetime. The use of a short column with a large diameter (6 × 1 cm) gave increased loading capacity above 400μg protein and enhanced recovery while maintaining good resolution. A less expensive reversed-phase support (17-μm C 18 ) provided adequate resolution for this separation." @default.
- W2090982662 created "2016-06-24" @default.
- W2090982662 creator A5064328398 @default.
- W2090982662 creator A5068769791 @default.
- W2090982662 date "1984-07-01" @default.
- W2090982662 modified "2023-09-26" @default.
- W2090982662 title "Micropreparative protein purification by reversed-phase high-performance liquid chromatography" @default.
- W2090982662 cites W1567804644 @default.
- W2090982662 cites W1574128379 @default.
- W2090982662 cites W1775749144 @default.
- W2090982662 cites W1965604856 @default.
- W2090982662 cites W1970775870 @default.
- W2090982662 cites W1998016614 @default.
- W2090982662 cites W2006084690 @default.
- W2090982662 cites W2023822557 @default.
- W2090982662 cites W2030469267 @default.
- W2090982662 cites W2042365395 @default.
- W2090982662 cites W2048923862 @default.
- W2090982662 cites W2054931113 @default.
- W2090982662 cites W2069041067 @default.
- W2090982662 cites W2073102532 @default.
- W2090982662 cites W2073879684 @default.
- W2090982662 cites W2079791947 @default.
- W2090982662 cites W2107617273 @default.
- W2090982662 cites W2108540409 @default.
- W2090982662 cites W2171923333 @default.
- W2090982662 cites W23570572 @default.
- W2090982662 cites W2413409309 @default.
- W2090982662 cites W2467932995 @default.
- W2090982662 cites W2896355008 @default.
- W2090982662 cites W4244714944 @default.
- W2090982662 cites W979425508 @default.
- W2090982662 doi "https://doi.org/10.1016/s0021-9673(01)96407-6" @default.
- W2090982662 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6480738" @default.
- W2090982662 hasPublicationYear "1984" @default.
- W2090982662 type Work @default.
- W2090982662 sameAs 2090982662 @default.
- W2090982662 citedByCount "17" @default.
- W2090982662 countsByYear W20909826622022 @default.
- W2090982662 crossrefType "journal-article" @default.
- W2090982662 hasAuthorship W2090982662A5064328398 @default.
- W2090982662 hasAuthorship W2090982662A5068769791 @default.
- W2090982662 hasConcept C113196181 @default.
- W2090982662 hasConcept C127313418 @default.
- W2090982662 hasConcept C134121241 @default.
- W2090982662 hasConcept C138268822 @default.
- W2090982662 hasConcept C150394285 @default.
- W2090982662 hasConcept C154945302 @default.
- W2090982662 hasConcept C178790620 @default.
- W2090982662 hasConcept C179998833 @default.
- W2090982662 hasConcept C185592680 @default.
- W2090982662 hasConcept C191897082 @default.
- W2090982662 hasConcept C192562407 @default.
- W2090982662 hasConcept C3020199158 @default.
- W2090982662 hasConcept C41008148 @default.
- W2090982662 hasConcept C43617362 @default.
- W2090982662 hasConcept C44280652 @default.
- W2090982662 hasConcept C62649853 @default.
- W2090982662 hasConceptScore W2090982662C113196181 @default.
- W2090982662 hasConceptScore W2090982662C127313418 @default.
- W2090982662 hasConceptScore W2090982662C134121241 @default.
- W2090982662 hasConceptScore W2090982662C138268822 @default.
- W2090982662 hasConceptScore W2090982662C150394285 @default.
- W2090982662 hasConceptScore W2090982662C154945302 @default.
- W2090982662 hasConceptScore W2090982662C178790620 @default.
- W2090982662 hasConceptScore W2090982662C179998833 @default.
- W2090982662 hasConceptScore W2090982662C185592680 @default.
- W2090982662 hasConceptScore W2090982662C191897082 @default.
- W2090982662 hasConceptScore W2090982662C192562407 @default.
- W2090982662 hasConceptScore W2090982662C3020199158 @default.
- W2090982662 hasConceptScore W2090982662C41008148 @default.
- W2090982662 hasConceptScore W2090982662C43617362 @default.
- W2090982662 hasConceptScore W2090982662C44280652 @default.
- W2090982662 hasConceptScore W2090982662C62649853 @default.
- W2090982662 hasLocation W20909826621 @default.
- W2090982662 hasLocation W20909826622 @default.
- W2090982662 hasOpenAccess W2090982662 @default.
- W2090982662 hasPrimaryLocation W20909826621 @default.
- W2090982662 hasRelatedWork W1967517180 @default.
- W2090982662 hasRelatedWork W2001774848 @default.
- W2090982662 hasRelatedWork W2046780476 @default.
- W2090982662 hasRelatedWork W2048793552 @default.
- W2090982662 hasRelatedWork W2065647693 @default.
- W2090982662 hasRelatedWork W2073360156 @default.
- W2090982662 hasRelatedWork W2076983111 @default.
- W2090982662 hasRelatedWork W2091343852 @default.
- W2090982662 hasRelatedWork W2166263961 @default.
- W2090982662 hasRelatedWork W2324004200 @default.
- W2090982662 hasVolume "296" @default.
- W2090982662 isParatext "false" @default.
- W2090982662 isRetracted "false" @default.
- W2090982662 magId "2090982662" @default.
- W2090982662 workType "article" @default.