FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090983251> ?p ?o ?g. }

• W2090983251 endingPage "100" @default.
• W2090983251 startingPage "93" @default.
• W2090983251 abstract "Recent studies have shown that Chinese hamster ovary (CHO) cells transfected with the FSH subunit genes secrete bioactive FSH. Here, we determined the in vitro and in vivo bioactivity of recombinant FSH produced by CHO mutant cells deficient in the glycosylation enzyme N-acetylglucosamine transferase-I (NAGT-), resulting in glycoproteins with asparagine-linked (GlcNAc)2(Mannose)5 oligosaccharides, or mutant cells defective in sialic acid transport into the Golgi (ST-). In the latter, glycoproteins are secreted lacking terminal sialic acids. Determination of in vitro bioactivity, using the granulosa cell aromatase bioassay, indicated that both FSH variants are as active as FSH secreted by the wild type (WT) cells and purified pituitary FSH. Also, these normal and variant forms of FSH are equipotent in a radioligand receptor assay using rat testis membranes. However, the variant FSH molecules are more basic than the WT FSH as determined using a chromatofocusing column (pI: wild type 3.6-5.0, NAGT- greater than 7.0, ST- approximately 6.0 and greater than 7.0). Injection of immature estrogen-treated rats with WT FSH induced high aromatase activity in their granulosa cells whereas treatment with either one of the FSH variants was ineffective; the lack of in vivo activity of the FSH variants was correlated with rapid clearance of these molecules in serum. Thus, recombinant human FSH produced by cells deficient in NAGT-I or defective in sialic acid transport retains normal receptor binding and in vitro bioactivity, but exhibits minimal in vivo activity and a shortened half-life when compared to WT FSH, indicating the important role of terminal sugars for FSH action in vivo." @default.
• W2090983251 created "2016-06-24" @default.
• W2090983251 creator A5006681163 @default.
• W2090983251 creator A5025270511 @default.
• W2090983251 creator A5054474479 @default.
• W2090983251 creator A5068525759 @default.
• W2090983251 creator A5077162723 @default.
• W2090983251 creator A5084902610 @default.
• W2090983251 date "1990-07-01" @default.
• W2090983251 modified "2023-10-14" @default.
• W2090983251 title "<i>In Vitro</i>and<i>in Vivo</i>Bioactivity of Recombinant Human Follicle-Stimulating Hormone and Partially Deglycosylated Variants Secreted by Transfected Eukaryotic Cell Lines*" @default.
• W2090983251 cites W1146978950 @default.
• W2090983251 cites W1518753545 @default.
• W2090983251 cites W1552293911 @default.
• W2090983251 cites W1552939650 @default.
• W2090983251 cites W1559504720 @default.
• W2090983251 cites W1565160468 @default.
• W2090983251 cites W1569589672 @default.
• W2090983251 cites W1573609576 @default.
• W2090983251 cites W1679692751 @default.
• W2090983251 cites W1973939186 @default.
• W2090983251 cites W1977116834 @default.
• W2090983251 cites W1982626008 @default.
• W2090983251 cites W1986185641 @default.
• W2090983251 cites W1990280547 @default.
• W2090983251 cites W1999208899 @default.
• W2090983251 cites W2005446108 @default.
• W2090983251 cites W2009378175 @default.
• W2090983251 cites W2011296435 @default.
• W2090983251 cites W2011977374 @default.
• W2090983251 cites W2019219968 @default.
• W2090983251 cites W2023767067 @default.
• W2090983251 cites W2031148940 @default.
• W2090983251 cites W2031192188 @default.
• W2090983251 cites W2038903137 @default.
• W2090983251 cites W2040453840 @default.
• W2090983251 cites W2057615981 @default.
• W2090983251 cites W2070927927 @default.
• W2090983251 cites W2074236250 @default.
• W2090983251 cites W2074343140 @default.
• W2090983251 cites W2078305907 @default.
• W2090983251 cites W2079563753 @default.
• W2090983251 cites W2080580789 @default.
• W2090983251 cites W2083771700 @default.
• W2090983251 cites W2086441018 @default.
• W2090983251 cites W2092771790 @default.
• W2090983251 cites W2094169235 @default.
• W2090983251 cites W2132786573 @default.
• W2090983251 cites W2149936339 @default.
• W2090983251 cites W2154638287 @default.
• W2090983251 cites W2161622051 @default.
• W2090983251 cites W2335950222 @default.
• W2090983251 cites W2412085295 @default.
• W2090983251 cites W31868260 @default.
• W2090983251 doi "https://doi.org/10.1210/endo-127-1-93" @default.
• W2090983251 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2141816" @default.
• W2090983251 hasPublicationYear "1990" @default.
• W2090983251 type Work @default.
• W2090983251 sameAs 2090983251 @default.
• W2090983251 citedByCount "104" @default.
• W2090983251 countsByYear W20909832512013 @default.
• W2090983251 countsByYear W20909832512014 @default.
• W2090983251 countsByYear W20909832512015 @default.
• W2090983251 countsByYear W20909832512016 @default.
• W2090983251 countsByYear W20909832512018 @default.
• W2090983251 countsByYear W20909832512019 @default.
• W2090983251 countsByYear W20909832512020 @default.
• W2090983251 countsByYear W20909832512021 @default.
• W2090983251 countsByYear W20909832512022 @default.
• W2090983251 crossrefType "journal-article" @default.
• W2090983251 hasAuthorship W2090983251A5006681163 @default.
• W2090983251 hasAuthorship W2090983251A5025270511 @default.
• W2090983251 hasAuthorship W2090983251A5054474479 @default.
• W2090983251 hasAuthorship W2090983251A5068525759 @default.
• W2090983251 hasAuthorship W2090983251A5077162723 @default.
• W2090983251 hasAuthorship W2090983251A5084902610 @default.
• W2090983251 hasConcept C108625454 @default.
• W2090983251 hasConcept C126322002 @default.
• W2090983251 hasConcept C134018914 @default.
• W2090983251 hasConcept C150903083 @default.
• W2090983251 hasConcept C153911025 @default.
• W2090983251 hasConcept C170493617 @default.
• W2090983251 hasConcept C175656101 @default.
• W2090983251 hasConcept C207001950 @default.
• W2090983251 hasConcept C2777313579 @default.
• W2090983251 hasConcept C2778575703 @default.
• W2090983251 hasConcept C2779279165 @default.
• W2090983251 hasConcept C2780115458 @default.
• W2090983251 hasConcept C29390026 @default.
• W2090983251 hasConcept C54355233 @default.
• W2090983251 hasConcept C55493867 @default.
• W2090983251 hasConcept C71315377 @default.
• W2090983251 hasConcept C71924100 @default.
• W2090983251 hasConcept C81885089 @default.
• W2090983251 hasConcept C86803240 @default.
• W2090983251 hasConceptScore W2090983251C108625454 @default.
• W2090983251 hasConceptScore W2090983251C126322002 @default.
• W2090983251 hasConceptScore W2090983251C134018914 @default.

• next