FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2090988310> ?p ?o ?g. }

• W2090988310 endingPage "123" @default.
• W2090988310 startingPage "123" @default.
• W2090988310 abstract "Somatic cell nuclear transfer (SCNT) has been proposed as an outstanding tool for expanding the dissemination capacity of animals of extreme genetic value, as well as for the genetic resurrection of elite animals affected by incurable disease or that died suddenly. Numbers of outstanding males of meat-specialised breeds of goats (Boer) and sheep (Dorper) recently imported into Argentina were expanded using SCNT technology. Oocytes were collected by laparoscopic ovum pickup (LOPU) from 40 Raza Criolla goats and 38 crossbreed sheep that were hormonally stimulated, as described previously (Baldassarre et al. 2002 Theriogenology 57, 275). Oocyte maturation, cell transfer, fusion and activation, culture, and transfer to recipients were conducted following procedures previously described (Baldassarre et al. 2003 Cloning Stem Cells 5, 279). Briefly, oocytes were matured in vitro for 24 h in TCM 199 supplemented with hormones and 10% serum, at 38.5°C and 5% CO2. 2 caprine fetal fibroblast cell lines (FF1 and FF2) were established from purebred Boer fetuses generated by selective breeding of elite animals, while a fibroblast ovine line was established from a skin biopsy from an elite Dorper ram. Cells were transferred into previously enucleated oocytes, followed by electric fusion using a single DC pulse of 1.6 kV cm–1 for 70 μs. Finally, the reconstructed embryos were activated using ionomycin (5 μM/5 min) followed by cycloheximide (10 μg mL–1) and cytochalasin B (7.5 μg mL–1) for 4 to 5 h, followed by in vitro culture in mSOF media before transfer into the oviducts of synchronized recipients within 24 h after fusion. An average of 10.4 and 12.8 reconstructed embryos were transferred to each of 21 and 12 recipient goats and sheep, respectively. Pregnancy was detected and monitored for the first 3 months by transrectal ultrasound scanning. Initial pregnancy (4 recipients, 33%) was maintained from gestation Day 30 to term in sheep, while goats exhibited a dramatic drop from 9 recipients pregnant (41%) on Day 30 to only 2 (9%) giving birth. Deliveries were by elective C-section. The number of normal offspring with good postpartum survival was 2/2 in goats (100%) and 3/5 (60%) in sheep. Substantial differences were observed between the 2 cell lines used in goats, where pregnancy was 4/11 (36%) for FF1 and 5/10 (50%) for FF2 at Day 30; however, only 2 goats carrying FF2 pregnancies carried. These results are in agreement with previous reports suggesting that cell line may be the largest source of result variation in SCNT. At the time of writing this abstract these clones are ∼4 months of age, healthy and growing normally (>40 kg weight). To the best of our knowledge, these are the first cloned goats produced by SCNT technology in Latin America, and the second group to produce cloned sheep in the region." @default.
• W2090988310 created "2016-06-24" @default.
• W2090988310 creator A5014540169 @default.
• W2090988310 creator A5014805538 @default.
• W2090988310 creator A5025667359 @default.
• W2090988310 creator A5027218649 @default.
• W2090988310 creator A5040771289 @default.
• W2090988310 creator A5046287778 @default.
• W2090988310 creator A5047415243 @default.
• W2090988310 creator A5050486589 @default.
• W2090988310 creator A5058243751 @default.
• W2090988310 creator A5072178805 @default.
• W2090988310 date "2011-01-01" @default.
• W2090988310 modified "2023-09-24" @default.
• W2090988310 title "33 PRODUCTION OF CLONED BOER GOATS AND DORPER SHEEP IN ARGENTINA" @default.
• W2090988310 doi "https://doi.org/10.1071/rdv23n1ab33" @default.
• W2090988310 hasPublicationYear "2011" @default.
• W2090988310 type Work @default.
• W2090988310 sameAs 2090988310 @default.
• W2090988310 citedByCount "2" @default.
• W2090988310 countsByYear W20909883102012 @default.
• W2090988310 countsByYear W20909883102014 @default.
• W2090988310 crossrefType "journal-article" @default.
• W2090988310 hasAuthorship W2090988310A5014540169 @default.
• W2090988310 hasAuthorship W2090988310A5014805538 @default.
• W2090988310 hasAuthorship W2090988310A5025667359 @default.
• W2090988310 hasAuthorship W2090988310A5027218649 @default.
• W2090988310 hasAuthorship W2090988310A5040771289 @default.
• W2090988310 hasAuthorship W2090988310A5046287778 @default.
• W2090988310 hasAuthorship W2090988310A5047415243 @default.
• W2090988310 hasAuthorship W2090988310A5050486589 @default.
• W2090988310 hasAuthorship W2090988310A5058243751 @default.
• W2090988310 hasAuthorship W2090988310A5072178805 @default.
• W2090988310 hasConcept C140793950 @default.
• W2090988310 hasConcept C147252523 @default.
• W2090988310 hasConcept C14814345 @default.
• W2090988310 hasConcept C16685009 @default.
• W2090988310 hasConcept C173419221 @default.
• W2090988310 hasConcept C179933525 @default.
• W2090988310 hasConcept C196843134 @default.
• W2090988310 hasConcept C202438428 @default.
• W2090988310 hasConcept C202751555 @default.
• W2090988310 hasConcept C2778177303 @default.
• W2090988310 hasConcept C2779012151 @default.
• W2090988310 hasConcept C2994278967 @default.
• W2090988310 hasConcept C39297687 @default.
• W2090988310 hasConcept C42972112 @default.
• W2090988310 hasConcept C54355233 @default.
• W2090988310 hasConcept C71924100 @default.
• W2090988310 hasConcept C86803240 @default.
• W2090988310 hasConcept C87073359 @default.
• W2090988310 hasConceptScore W2090988310C140793950 @default.
• W2090988310 hasConceptScore W2090988310C147252523 @default.
• W2090988310 hasConceptScore W2090988310C14814345 @default.
• W2090988310 hasConceptScore W2090988310C16685009 @default.
• W2090988310 hasConceptScore W2090988310C173419221 @default.
• W2090988310 hasConceptScore W2090988310C179933525 @default.
• W2090988310 hasConceptScore W2090988310C196843134 @default.
• W2090988310 hasConceptScore W2090988310C202438428 @default.
• W2090988310 hasConceptScore W2090988310C202751555 @default.
• W2090988310 hasConceptScore W2090988310C2778177303 @default.
• W2090988310 hasConceptScore W2090988310C2779012151 @default.
• W2090988310 hasConceptScore W2090988310C2994278967 @default.
• W2090988310 hasConceptScore W2090988310C39297687 @default.
• W2090988310 hasConceptScore W2090988310C42972112 @default.
• W2090988310 hasConceptScore W2090988310C54355233 @default.
• W2090988310 hasConceptScore W2090988310C71924100 @default.
• W2090988310 hasConceptScore W2090988310C86803240 @default.
• W2090988310 hasConceptScore W2090988310C87073359 @default.
• W2090988310 hasIssue "1" @default.
• W2090988310 hasLocation W20909883101 @default.
• W2090988310 hasOpenAccess W2090988310 @default.
• W2090988310 hasPrimaryLocation W20909883101 @default.
• W2090988310 hasRelatedWork W1531522704 @default.
• W2090988310 hasRelatedWork W1567668280 @default.
• W2090988310 hasRelatedWork W2002769091 @default.
• W2090988310 hasRelatedWork W2004306661 @default.
• W2090988310 hasRelatedWork W2007826443 @default.
• W2090988310 hasRelatedWork W2053101402 @default.
• W2090988310 hasRelatedWork W2071557794 @default.
• W2090988310 hasRelatedWork W2088431763 @default.
• W2090988310 hasRelatedWork W2090578102 @default.
• W2090988310 hasRelatedWork W2090988310 @default.
• W2090988310 hasVolume "23" @default.
• W2090988310 isParatext "false" @default.
• W2090988310 isRetracted "false" @default.
• W2090988310 magId "2090988310" @default.
• W2090988310 workType "article" @default.