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• W2090996413 abstract "Time-resolved emission as well as steady-state UV-vis techniques were employed to study the photoprotolytic processes that d-luciferin, the natural substrate of the firefly luciferase, undergoes in both acidic aqueous solutions and ice. The emission spectrum of D-luciferin in a 20 mM HCl aqueous solution or higher has an additional emission band at 590 nm red-shifted with respect to the strongest emission band positioned at 530 nm of the deprotonated NRO(-*) form in a pH-neutral aqueous solution. We attribute this emission band to the zwitterion form designated as (+)HNRO(-). The time-resolved emission signals show that the NRO(-*) emission band at 530 nm and the zwitterion emission band at 590 are strongly quenched by a recombination process with a proton in an acidic solution and in ice. In ice, the quenching rate is 10 times faster than in the liquid state. We attribute the fast quenching rate to the high value of the proton diffusion constant in ice." @default.
• W2090996413 created "2016-06-24" @default.
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• W2090996413 date "2011-02-16" @default.
• W2090996413 modified "2023-10-02" @default.
• W2090996413 title "Excited-State Intermolecular Proton Transfer of Firefly Luciferin IV. Temperature and pH Dependence" @default.
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• W2090996413 doi "https://doi.org/10.1021/jp110889v" @default.
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