FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091006988> ?p ?o ?g. }

• W2091006988 endingPage "5470" @default.
• W2091006988 startingPage "5458" @default.
• W2091006988 abstract "ABSTRACT CtrA controls cell cycle programs of chromosome replication and genetic transcription. Phosphorylated CtrA∼P exhibits high affinity (dissociation constant [ K d ], <10 nM) for consensus TTAA-N7-TTAA binding sites with “typical” (N = 7) spacing. We show here that ctrA promoters P1 and P2 use low-affinity ( K d , >500 nM) CtrA binding sites with “atypical” (N ≠ 7) spacing. Footprints demonstrated that phosphorylated CtrA∼P does not exhibit increased affinity for “atypical” sites, as it does for sites in the replication origin. Instead, high levels of CtrA (>10 μM) accumulate, which can drive CtrA binding to “atypical” sites. In vivo cross-linking showed that when the stable CtrAΔ3 protein persists during the cell cycle, the “atypical” sites at ctrA and motB are persistently bound. Interestingly, the cell cycle timing of ctrA P1 and P2 transcription is not altered by persistent CtrAΔ3 binding. Therefore, operator DNA occupancy is not sufficient for regulation, and it is the cell cycle variation of CtrA∼P phosphorylation that provides the dominant “activation” signal. Protein dimerization is one potential means of “activation.” The glutathione S -transferase (GST) protein dimerizes, and fusion with CtrA (GST-CtrA) creates a stable dimer with enhanced affinity for TTAA motifs. Electrophoretic mobility shift assays with GST-CtrA revealed cooperative modes of binding that further distinguish the “atypical” sites. GST-CtrA also binds a single TTAA motif in ctrA P1 aided by DNA in the extended TTAACCAT motif. We discuss how “atypical” sites are a common yet distinct category of CtrA regulatory sites and new implications for the working and evolution of cell cycle control networks." @default.
• W2091006988 created "2016-06-24" @default.
• W2091006988 creator A5041830225 @default.
• W2091006988 creator A5055109324 @default.
• W2091006988 creator A5067542579 @default.
• W2091006988 creator A5067743256 @default.
• W2091006988 creator A5079346410 @default.
• W2091006988 date "2009-09-01" @default.
• W2091006988 modified "2023-09-30" @default.
• W2091006988 title "CtrA, a Global Response Regulator, Uses a Distinct Second Category of Weak DNA Binding Sites for Cell Cycle Transcription Control in <i>Caulobacter crescentus</i>" @default.
• W2091006988 cites W1755317122 @default.
• W2091006988 cites W1919046875 @default.
• W2091006988 cites W1963867445 @default.
• W2091006988 cites W1965456430 @default.
• W2091006988 cites W1976110793 @default.
• W2091006988 cites W1982227919 @default.
• W2091006988 cites W1985253447 @default.
• W2091006988 cites W1987637706 @default.
• W2091006988 cites W1999224877 @default.
• W2091006988 cites W2006002169 @default.
• W2091006988 cites W2006832626 @default.
• W2091006988 cites W2007340952 @default.
• W2091006988 cites W2027229485 @default.
• W2091006988 cites W2034266027 @default.
• W2091006988 cites W2034750183 @default.
• W2091006988 cites W2035135843 @default.
• W2091006988 cites W2041594648 @default.
• W2091006988 cites W2049076326 @default.
• W2091006988 cites W2057460911 @default.
• W2091006988 cites W2058681460 @default.
• W2091006988 cites W2064354495 @default.
• W2091006988 cites W2073169023 @default.
• W2091006988 cites W2081104716 @default.
• W2091006988 cites W2082544747 @default.
• W2091006988 cites W2082873640 @default.
• W2091006988 cites W2087950896 @default.
• W2091006988 cites W2088880573 @default.
• W2091006988 cites W2089947295 @default.
• W2091006988 cites W2091786047 @default.
• W2091006988 cites W2097896589 @default.
• W2091006988 cites W2118912103 @default.
• W2091006988 cites W2125376264 @default.
• W2091006988 cites W2129550156 @default.
• W2091006988 cites W2131288254 @default.
• W2091006988 cites W2132150887 @default.
• W2091006988 cites W2132481315 @default.
• W2091006988 cites W2136716105 @default.
• W2091006988 cites W950515816 @default.
• W2091006988 doi "https://doi.org/10.1128/jb.00355-09" @default.
• W2091006988 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2725627" @default.
• W2091006988 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19542275" @default.
• W2091006988 hasPublicationYear "2009" @default.
• W2091006988 type Work @default.
• W2091006988 sameAs 2091006988 @default.
• W2091006988 citedByCount "39" @default.
• W2091006988 countsByYear W20910069882012 @default.
• W2091006988 countsByYear W20910069882013 @default.
• W2091006988 countsByYear W20910069882014 @default.
• W2091006988 countsByYear W20910069882015 @default.
• W2091006988 countsByYear W20910069882017 @default.
• W2091006988 countsByYear W20910069882018 @default.
• W2091006988 countsByYear W20910069882019 @default.
• W2091006988 countsByYear W20910069882020 @default.
• W2091006988 countsByYear W20910069882021 @default.
• W2091006988 countsByYear W20910069882022 @default.
• W2091006988 crossrefType "journal-article" @default.
• W2091006988 hasAuthorship W2091006988A5041830225 @default.
• W2091006988 hasAuthorship W2091006988A5055109324 @default.
• W2091006988 hasAuthorship W2091006988A5067542579 @default.
• W2091006988 hasAuthorship W2091006988A5067743256 @default.
• W2091006988 hasAuthorship W2091006988A5079346410 @default.
• W2091006988 hasBestOaLocation W20910069881 @default.
• W2091006988 hasConcept C104317684 @default.
• W2091006988 hasConcept C138885662 @default.
• W2091006988 hasConcept C1491633281 @default.
• W2091006988 hasConcept C153911025 @default.
• W2091006988 hasConcept C179926584 @default.
• W2091006988 hasConcept C2778767192 @default.
• W2091006988 hasConcept C29537977 @default.
• W2091006988 hasConcept C41895202 @default.
• W2091006988 hasConcept C54355233 @default.
• W2091006988 hasConcept C552990157 @default.
• W2091006988 hasConcept C73573662 @default.
• W2091006988 hasConcept C86339819 @default.
• W2091006988 hasConcept C86803240 @default.
• W2091006988 hasConcept C94966510 @default.
• W2091006988 hasConcept C95444343 @default.
• W2091006988 hasConceptScore W2091006988C104317684 @default.
• W2091006988 hasConceptScore W2091006988C138885662 @default.
• W2091006988 hasConceptScore W2091006988C1491633281 @default.
• W2091006988 hasConceptScore W2091006988C153911025 @default.
• W2091006988 hasConceptScore W2091006988C179926584 @default.
• W2091006988 hasConceptScore W2091006988C2778767192 @default.
• W2091006988 hasConceptScore W2091006988C29537977 @default.
• W2091006988 hasConceptScore W2091006988C41895202 @default.
• W2091006988 hasConceptScore W2091006988C54355233 @default.
• W2091006988 hasConceptScore W2091006988C552990157 @default.
• W2091006988 hasConceptScore W2091006988C73573662 @default.

• next