FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091201148> ?p ?o ?g. }

• W2091201148 endingPage "255" @default.
• W2091201148 startingPage "245" @default.
• W2091201148 abstract "Sirtuin1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD(+))-dependent deacetylase functioning in the regulation of metabolism, cell survival and organismal lifespan. Active SIRT1 regulates autophagy during cell stress, including calorie restriction, endoplasmic reticulum (ER) stress and oxidative stress. Previously, we reported that fluoride induces ER-stress in ameloblasts responsible for enamel formation, suggesting that ER-stress plays a role in dental fluorosis. However, the molecular mechanism of how cells respond to fluoride-induced cell stress is unclear. Here, we demonstrate that fluoride activates SIRT1 and initiates autophagy to protect cells from fluoride exposure. Fluoride treatment of ameloblast-derived cells (LS8) significantly increased Sirt1 expression and induced SIRT1 phosphorylation resulting in the augmentation of SIRT1 deacetylase activity. To demonstrate that fluoride exposure initiates autophagy, we characterized the expression of autophagy related genes (Atg); Atg5, Atg7 and Atg8/LC3 and showed that both their transcript and protein levels were significantly increased following fluoride treatment. To confirm that SIRT1 plays a protective role in fluoride toxicity, we used resveratrol (RES) to augment SIRT1 activity in fluoride treated LS8 cells. RES increased autophagy, inhibited apoptosis, and decreased fluoride cytotoxicity. Rats treated with fluoride (0, 50, 100 and 125ppm) in drinking water for 6weeks had significantly elevated expression levels of Sirt1, Atg5, Atg7 and Atg8/LC3 in their maturation stage enamel organs. Increased protein levels of p-SIRT1, ATG5 and ATG8/LC3 were present in fluoride-treated rat maturation stage ameloblasts. Therefore, the SIRT1/autophagy pathway may play a critical role as a protective response to help prevent dental fluorosis." @default.
• W2091201148 created "2016-06-24" @default.
• W2091201148 creator A5001606188 @default.
• W2091201148 creator A5006600622 @default.
• W2091201148 date "2014-02-01" @default.
• W2091201148 modified "2023-09-27" @default.
• W2091201148 title "Sirtuin1 and autophagy protect cells from fluoride-induced cell stress" @default.
• W2091201148 cites W1535295956 @default.
• W2091201148 cites W1792467926 @default.
• W2091201148 cites W1850028273 @default.
• W2091201148 cites W1964483580 @default.
• W2091201148 cites W1967901428 @default.
• W2091201148 cites W1968608498 @default.
• W2091201148 cites W1971448178 @default.
• W2091201148 cites W1973303893 @default.
• W2091201148 cites W1974890383 @default.
• W2091201148 cites W1976531450 @default.
• W2091201148 cites W1976881265 @default.
• W2091201148 cites W1978069733 @default.
• W2091201148 cites W1978550818 @default.
• W2091201148 cites W1978936329 @default.
• W2091201148 cites W1979075010 @default.
• W2091201148 cites W1980472251 @default.
• W2091201148 cites W1985247208 @default.
• W2091201148 cites W1987178054 @default.
• W2091201148 cites W1987956328 @default.
• W2091201148 cites W1989051519 @default.
• W2091201148 cites W1992357172 @default.
• W2091201148 cites W2004503111 @default.
• W2091201148 cites W2007842741 @default.
• W2091201148 cites W2008224422 @default.
• W2091201148 cites W2015913494 @default.
• W2091201148 cites W2016040396 @default.
• W2091201148 cites W2017525855 @default.
• W2091201148 cites W2020771162 @default.
• W2091201148 cites W2023692489 @default.
• W2091201148 cites W2024782183 @default.
• W2091201148 cites W2030936504 @default.
• W2091201148 cites W2031169909 @default.
• W2091201148 cites W2031213396 @default.
• W2091201148 cites W2032213666 @default.
• W2091201148 cites W2035859053 @default.
• W2091201148 cites W2039677504 @default.
• W2091201148 cites W2041959419 @default.
• W2091201148 cites W2044176668 @default.
• W2091201148 cites W2048856016 @default.
• W2091201148 cites W2048906289 @default.
• W2091201148 cites W2058412892 @default.
• W2091201148 cites W2062350887 @default.
• W2091201148 cites W2062843720 @default.
• W2091201148 cites W2063322789 @default.
• W2091201148 cites W2067644861 @default.
• W2091201148 cites W2070815033 @default.
• W2091201148 cites W2071221374 @default.
• W2091201148 cites W2072022268 @default.
• W2091201148 cites W2073988218 @default.
• W2091201148 cites W2074851554 @default.
• W2091201148 cites W2077165816 @default.
• W2091201148 cites W2078575160 @default.
• W2091201148 cites W2080440554 @default.
• W2091201148 cites W2080901528 @default.
• W2091201148 cites W2087021020 @default.
• W2091201148 cites W2087232165 @default.
• W2091201148 cites W2088381487 @default.
• W2091201148 cites W2092753187 @default.
• W2091201148 cites W2093394803 @default.
• W2091201148 cites W2094851471 @default.
• W2091201148 cites W2096308078 @default.
• W2091201148 cites W2097948853 @default.
• W2091201148 cites W2102614059 @default.
• W2091201148 cites W2102646072 @default.
• W2091201148 cites W2103596198 @default.
• W2091201148 cites W2104316109 @default.
• W2091201148 cites W2108244474 @default.
• W2091201148 cites W2114957061 @default.
• W2091201148 cites W2115205812 @default.
• W2091201148 cites W2115575149 @default.
• W2091201148 cites W2115888489 @default.
• W2091201148 cites W2117119983 @default.
• W2091201148 cites W2123700755 @default.
• W2091201148 cites W2123927456 @default.
• W2091201148 cites W2125121177 @default.
• W2091201148 cites W2129777487 @default.
• W2091201148 cites W2133000461 @default.
• W2091201148 cites W2135184057 @default.
• W2091201148 cites W2146415848 @default.
• W2091201148 cites W2151089147 @default.
• W2091201148 cites W2156453610 @default.
• W2091201148 cites W2157096314 @default.
• W2091201148 cites W2157860216 @default.
• W2091201148 cites W2164038572 @default.
• W2091201148 cites W2167656141 @default.
• W2091201148 cites W2167990179 @default.
• W2091201148 cites W2168987076 @default.
• W2091201148 cites W2171334003 @default.
• W2091201148 cites W2792139654 @default.
• W2091201148 doi "https://doi.org/10.1016/j.bbadis.2013.11.023" @default.
• W2091201148 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3913072" @default.

• next