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- W2091209814 abstract "During the last few decades, it has become evident that the compatibility of the yeast biochemical environment with the ability to process and translate the RNA transcript, along with its capacity to modify a translated protein, are relevant requirements for selecting this host cell for protein expression in several pharmaceutical and clinical applications. In particular, Pichia pastoris is used as an industrial host for recombinant protein and metabolite production, showing a powerful capacity to meet required biomolecular target production levels in high-throughput assays for functional genomics and drug screening. In addition, there is a great advantage to using P. pastoris for protein secretion, even at high molecular weights, since the recovery and purification steps are simplified owing to relatively low levels of endogenous proteins in the extracellular medium. Clearly, no single microexpression system can provide all of the desired properties for human protein production. Moreover, chemical and physical bioprocess parameters, including culture medium formulation, temperature, pH, agitation, aeration rates, induction, and feeding strategies, can highly influence product yield and quality. In order to benefit from the currently available wide range of biosynthesis strategies using P. pastoris, this mini review focuses on the developments and technological fermentation achievements, providing both a comparative and an overall integration analysis. The main aim is to highlight the relevance and versatility of the P. pastoris biosystem to the design of more cost-effective microfactories to meet the increasing demands for recombinant membrane proteins and clinical antibodies for several therapeutic applications." @default.
- W2091209814 created "2016-06-24" @default.
- W2091209814 creator A5002377476 @default.
- W2091209814 creator A5049252324 @default.
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- W2091209814 creator A5060607126 @default.
- W2091209814 creator A5083940858 @default.
- W2091209814 creator A5086343541 @default.
- W2091209814 date "2013-05-01" @default.
- W2091209814 modified "2023-10-16" @default.
- W2091209814 title "Pichia pastoris: A Recombinant Microfactory for Antibodies and Human Membrane Proteins" @default.
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