FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091210644> ?p ?o ?g. }

• W2091210644 endingPage "12200" @default.
• W2091210644 startingPage "12189" @default.
• W2091210644 abstract "Gene expression microarrays are widely used to investigate molecular targets in cancers, including lung cancer. In this study, we analyzed online non-small cell lung cancer (NSCLC) microarray databases, to screen the key genes and pathways related to NSCLC by bioinformatics analyses. And then, the expression levels of two selected genes in the down-regulated co-pathways, myosin light chain kinase (MYLK) and myosin regulatory light chain 9 (MYL9), were determined in tumor, paired paraneoplastic, and normal lung tissues. First, gene set enrichment analysis and meta-analysis were conducted to identify key genes and pathways that contribute to NSCLC carcinogenesis. Second, using the total RNA and protein extracted from lung cancer tissues (n = 240), adjacent non-cancer tissues (n = 240), and normal lung tissues (n = 300), we examined the MYLK and MYL9 expression levels by quantitative real-time PCR and Western blot. Finally, we explored the correlations between mRNA and protein expressions of these two genes and the clinicopathological parameters of NSCLC. Fifteen up-regulated and nine down-regulated co-pathways were observed. A number of differentially expressed genes (CALM1, THBS1, CSF3, BMP2, IL6ST, MYLK, ROCK2, IL3RA, MYL9, PPP2CA, CSF2RB, CNAQ, GRIA2, IL10RA, IL10RB, IL11RA, LIFR, PLCB4, and RAC3) were identified (P < 0.01) in the down-regulated co-pathways. The expression levels of MYLK and MYL9, which act downstream of the vascular smooth muscle contraction signal pathway and focal adhesion pathway, were significantly lower in cancer tissue than those in the paraneoplastic and normal tissues (P < 0.05). Moreover, the expression levels of these two genes in stages III and IV NSCLC were significantly increased, when compared to stages I and II, and expressions levels in NSCLC with lymphatic metastasis were higher than that without lymphatic metastasis (P < 0.05). Additionally, significant lower expression levels of the two genes were found in smokers than in nonsmokers (P < 0.05). In contrast, gender, differentiated degrees, and pathohistological type appeared to have no impact on these gene expressions (P > 0.05). These findings suggested that low MYLK and MYL9 expressions might be associated with the development of NSCLC. These genes may be also relevant to NSCLC metastasis. Future investigations with large sample sizes needed to verify these findings." @default.
• W2091210644 created "2016-06-24" @default.
• W2091210644 creator A5025452176 @default.
• W2091210644 creator A5060827269 @default.
• W2091210644 date "2014-09-02" @default.
• W2091210644 modified "2023-10-02" @default.
• W2091210644 title "MYLK and MYL9 expression in non-small cell lung cancer identified by bioinformatics analysis of public expression data" @default.
• W2091210644 cites W1967370907 @default.
• W2091210644 cites W1967594212 @default.
• W2091210644 cites W1970708612 @default.
• W2091210644 cites W1973386532 @default.
• W2091210644 cites W1975900611 @default.
• W2091210644 cites W1995653433 @default.
• W2091210644 cites W1996516526 @default.
• W2091210644 cites W2010111184 @default.
• W2091210644 cites W2018699957 @default.
• W2091210644 cites W2020126643 @default.
• W2091210644 cites W2022171721 @default.
• W2091210644 cites W2025937220 @default.
• W2091210644 cites W2034341872 @default.
• W2091210644 cites W2043904301 @default.
• W2091210644 cites W2053965379 @default.
• W2091210644 cites W2057079310 @default.
• W2091210644 cites W2060710804 @default.
• W2091210644 cites W2063825596 @default.
• W2091210644 cites W2064033691 @default.
• W2091210644 cites W2072748470 @default.
• W2091210644 cites W2077532089 @default.
• W2091210644 cites W2080248100 @default.
• W2091210644 cites W2081098333 @default.
• W2091210644 cites W2081436697 @default.
• W2091210644 cites W2091309870 @default.
• W2091210644 cites W2097238709 @default.
• W2091210644 cites W2106787323 @default.
• W2091210644 cites W2115628853 @default.
• W2091210644 cites W2116634113 @default.
• W2091210644 cites W2123605899 @default.
• W2091210644 cites W2130410032 @default.
• W2091210644 cites W2134603143 @default.
• W2091210644 cites W2136828863 @default.
• W2091210644 cites W2138742704 @default.
• W2091210644 cites W2140983290 @default.
• W2091210644 cites W2154362705 @default.
• W2091210644 cites W2154766134 @default.
• W2091210644 cites W2157411387 @default.
• W2091210644 cites W2159482845 @default.
• W2091210644 cites W2159907132 @default.
• W2091210644 cites W2160697532 @default.
• W2091210644 cites W2163694152 @default.
• W2091210644 cites W2164954378 @default.
• W2091210644 cites W2167060203 @default.
• W2091210644 cites W2168340241 @default.
• W2091210644 cites W2170989872 @default.
• W2091210644 cites W4250995405 @default.
• W2091210644 cites W4294216483 @default.
• W2091210644 doi "https://doi.org/10.1007/s13277-014-2527-3" @default.
• W2091210644 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25179839" @default.
• W2091210644 hasPublicationYear "2014" @default.
• W2091210644 type Work @default.
• W2091210644 sameAs 2091210644 @default.
• W2091210644 citedByCount "53" @default.
• W2091210644 countsByYear W20912106442014 @default.
• W2091210644 countsByYear W20912106442015 @default.
• W2091210644 countsByYear W20912106442016 @default.
• W2091210644 countsByYear W20912106442017 @default.
• W2091210644 countsByYear W20912106442018 @default.
• W2091210644 countsByYear W20912106442019 @default.
• W2091210644 countsByYear W20912106442020 @default.
• W2091210644 countsByYear W20912106442021 @default.
• W2091210644 countsByYear W20912106442022 @default.
• W2091210644 countsByYear W20912106442023 @default.
• W2091210644 crossrefType "journal-article" @default.
• W2091210644 hasAuthorship W2091210644A5025452176 @default.
• W2091210644 hasAuthorship W2091210644A5060827269 @default.
• W2091210644 hasConcept C104317684 @default.
• W2091210644 hasConcept C121608353 @default.
• W2091210644 hasConcept C142724271 @default.
• W2091210644 hasConcept C150194340 @default.
• W2091210644 hasConcept C153911025 @default.
• W2091210644 hasConcept C18431079 @default.
• W2091210644 hasConcept C2776256026 @default.
• W2091210644 hasConcept C502942594 @default.
• W2091210644 hasConcept C54355233 @default.
• W2091210644 hasConcept C555283112 @default.
• W2091210644 hasConcept C71924100 @default.
• W2091210644 hasConcept C86803240 @default.
• W2091210644 hasConceptScore W2091210644C104317684 @default.
• W2091210644 hasConceptScore W2091210644C121608353 @default.
• W2091210644 hasConceptScore W2091210644C142724271 @default.
• W2091210644 hasConceptScore W2091210644C150194340 @default.
• W2091210644 hasConceptScore W2091210644C153911025 @default.
• W2091210644 hasConceptScore W2091210644C18431079 @default.
• W2091210644 hasConceptScore W2091210644C2776256026 @default.
• W2091210644 hasConceptScore W2091210644C502942594 @default.
• W2091210644 hasConceptScore W2091210644C54355233 @default.
• W2091210644 hasConceptScore W2091210644C555283112 @default.
• W2091210644 hasConceptScore W2091210644C71924100 @default.
• W2091210644 hasConceptScore W2091210644C86803240 @default.

• next