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- W2091212977 abstract "The Escherichia coli RNA phage Qβ coat protein-encoding gene (C) was amplified from native Qβ RNA using a reverse transcription-PCR technique. Gene C contains sequences coding for both the 133-amino acid (aa) Qβ coat protein (CP) and the 329-aa read-through protein (A1) consisting of CP and an additional 196-aa C-terminal sequence, separated from CP within the C gene by an opal (UGA) stop codon. Primers ensuring the natural environment for gene C, especially within the ribosome-binding site, and supplying C with unique restriction sites at both ends have been prepared. An amplified 1062-bp PCR fragment was positioned under the control of the strong E. coli trp promoter (Ptrp) within a pGEM-derived plasmid. The synthesis of gene C products was confirmed electrophoretically and immunologically. An immunodiffusion test with anti-Qβ phage antibodies and electron microscopy evaluation of the purified recombinant products showed that when expressed, the Qβ C gene was responsible for high-level synthesis and correct self-assembly of Qβ CP monomers into capsids indistinguishable morphologically and immunologically from Qβ phage particles, which we plan to use as surface display vectors." @default.
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- W2091212977 date "1993-12-01" @default.
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- W2091212977 title "Recombinant rna phage qβ capsid particles synthesized and self-assembled in escherichia coli" @default.
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- W2091212977 doi "https://doi.org/10.1016/0378-1119(93)90261-z" @default.
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