FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091214882> ?p ?o ?g. }

• W2091214882 endingPage "5400" @default.
• W2091214882 startingPage "5400" @default.
• W2091214882 abstract "Genetic modification of cells before transplantation may allow the delivery of neuroprotective and other functional molecules to patients with neurodegenerative diseases. To avoid complications associated with virally transfected cells, we have explored the use of nonviral methods to insert genetic material into RPE cells.After transfection with plasmids encoding different pigment epithelium-derived factor (PEDF) fusion proteins, transfected cells were established and passaged up to 100 times. Gene expression of PEDF, ZnT3, ZIP2, CRALBP, CATD, and ZO-1 was determined by RT-PCR. Secretion dynamics were analyzed using ELISA and a spheroid-based assay was used to confirm the anti-angiogenic activity of the recombinant PEDF.Transfection efficiency reached up to 98.7% with a plasmid encoding PEDF and enhanced green fluorescent protein (EGFP) separately and 87.2% with a plasmid encoding an EGFP-PEDF fusion. Immunoblotting revealed that transfected RPE cells express the appropriate PEDF or EGFP-PEDF. Expression of recombinant PEDF is stable, as shown by its secretion for the 2 years and the 100 passages the cells have been followed. PEDF expression was overexpressed and the transfected cells exhibited increased proliferation, up-regulation of ZnT3 and ZIP2, and inhibited sprouting in human umbilical vein endothelial cell spheroids.Genetic in vitro modification of pigment epithelial cells using nonviral transfection protocols should improve the potential therapeutic treatment of neurodegenerative diseases by transplantation of genetically modified cells without the disadvantages of virally mediated transfection. Here we have shown that genetically modified RPE cells overexpress a functional human recombinant PEDF, as evidenced by the autogenic regulation of proliferation, up-regulation of two distinct zinc transporters, and in vitro inhibition of endothelial cell sprouting." @default.
• W2091214882 created "2016-06-24" @default.
• W2091214882 creator A5010176556 @default.
• W2091214882 creator A5014695135 @default.
• W2091214882 creator A5024707203 @default.
• W2091214882 creator A5029320277 @default.
• W2091214882 creator A5051604741 @default.
• W2091214882 creator A5060718513 @default.
• W2091214882 creator A5086807533 @default.
• W2091214882 date "2011-07-21" @default.
• W2091214882 modified "2023-09-27" @default.
• W2091214882 title "Endogenic Regulation of Proliferation and Zinc Transporters by Pigment Epithelial Cells Nonvirally Transfected with PEDF" @default.
• W2091214882 cites W1546497025 @default.
• W2091214882 cites W1802218256 @default.
• W2091214882 cites W1967627658 @default.
• W2091214882 cites W1968543521 @default.
• W2091214882 cites W1974571183 @default.
• W2091214882 cites W1981313461 @default.
• W2091214882 cites W1984142967 @default.
• W2091214882 cites W1985648029 @default.
• W2091214882 cites W1986991414 @default.
• W2091214882 cites W1992257311 @default.
• W2091214882 cites W1993949890 @default.
• W2091214882 cites W1995270215 @default.
• W2091214882 cites W2000663727 @default.
• W2091214882 cites W2014280596 @default.
• W2091214882 cites W2022084143 @default.
• W2091214882 cites W2040180386 @default.
• W2091214882 cites W2048126892 @default.
• W2091214882 cites W2056553905 @default.
• W2091214882 cites W2058775052 @default.
• W2091214882 cites W2063278513 @default.
• W2091214882 cites W2064338219 @default.
• W2091214882 cites W2075984382 @default.
• W2091214882 cites W2077067955 @default.
• W2091214882 cites W2077891356 @default.
• W2091214882 cites W2078908895 @default.
• W2091214882 cites W2080030418 @default.
• W2091214882 cites W2081069085 @default.
• W2091214882 cites W2085442615 @default.
• W2091214882 cites W2100837269 @default.
• W2091214882 cites W2106621005 @default.
• W2091214882 cites W2107277218 @default.
• W2091214882 cites W2108244474 @default.
• W2091214882 cites W2110746342 @default.
• W2091214882 cites W2119967302 @default.
• W2091214882 cites W2123698501 @default.
• W2091214882 cites W2137222303 @default.
• W2091214882 cites W2138339712 @default.
• W2091214882 cites W2138825607 @default.
• W2091214882 cites W2141324738 @default.
• W2091214882 cites W2141692468 @default.
• W2091214882 cites W2165066800 @default.
• W2091214882 cites W2168215545 @default.
• W2091214882 cites W2410926762 @default.
• W2091214882 cites W2411928312 @default.
• W2091214882 cites W308972795 @default.
• W2091214882 doi "https://doi.org/10.1167/iovs.10-6178" @default.
• W2091214882 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21508100" @default.
• W2091214882 hasPublicationYear "2011" @default.
• W2091214882 type Work @default.
• W2091214882 sameAs 2091214882 @default.
• W2091214882 citedByCount "5" @default.
• W2091214882 countsByYear W20912148822012 @default.
• W2091214882 countsByYear W20912148822013 @default.
• W2091214882 countsByYear W20912148822018 @default.
• W2091214882 countsByYear W20912148822021 @default.
• W2091214882 countsByYear W20912148822023 @default.
• W2091214882 crossrefType "journal-article" @default.
• W2091214882 hasAuthorship W2091214882A5010176556 @default.
• W2091214882 hasAuthorship W2091214882A5014695135 @default.
• W2091214882 hasAuthorship W2091214882A5024707203 @default.
• W2091214882 hasAuthorship W2091214882A5029320277 @default.
• W2091214882 hasAuthorship W2091214882A5051604741 @default.
• W2091214882 hasAuthorship W2091214882A5060718513 @default.
• W2091214882 hasAuthorship W2091214882A5086807533 @default.
• W2091214882 hasConcept C104317684 @default.
• W2091214882 hasConcept C123894998 @default.
• W2091214882 hasConcept C141071460 @default.
• W2091214882 hasConcept C142613039 @default.
• W2091214882 hasConcept C153911025 @default.
• W2091214882 hasConcept C2780394083 @default.
• W2091214882 hasConcept C2911091166 @default.
• W2091214882 hasConcept C40767141 @default.
• W2091214882 hasConcept C49039625 @default.
• W2091214882 hasConcept C502942594 @default.
• W2091214882 hasConcept C52237779 @default.
• W2091214882 hasConcept C54009773 @default.
• W2091214882 hasConcept C54355233 @default.
• W2091214882 hasConcept C55493867 @default.
• W2091214882 hasConcept C71924100 @default.
• W2091214882 hasConcept C81885089 @default.
• W2091214882 hasConcept C86803240 @default.
• W2091214882 hasConcept C95444343 @default.
• W2091214882 hasConceptScore W2091214882C104317684 @default.
• W2091214882 hasConceptScore W2091214882C123894998 @default.
• W2091214882 hasConceptScore W2091214882C141071460 @default.
• W2091214882 hasConceptScore W2091214882C142613039 @default.

• next