Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091219353> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2091219353 endingPage "804" @default.
- W2091219353 startingPage "793" @default.
- W2091219353 abstract "Abstract An easy method for preparation of bare cell nuclei from fresh solid tissues for DNA flow cytometry is described. Pieces of up to 2 × 2 × 2 mm 3 size from fresh tissues were fixed in formalin. After removal of formalin by washing with ethanol and rehydration with tap water, the tissue pieces were incubated with subtilisin Carlsberg (pronase, Sigma protease XXIV) and then stained directly with DAPI. Staining with ethidium bromide gave unsatisfactory results. Neither mechanical disaggregation nor centrifugation were used. The resulting cell nucleus suspensions had extremely low frequencies of debris particles and of clumped cell nuclei. A good yield, a minimized loss, and a good stainability of cell nuclei were obtained. The applicability of the method was exemplified by the analysis of biopsies from the colon‐rectum in patients with ulcerative colitis and of biopsies from the bladder in patients with bladder cancer and compared to the standard method of this laboratory, which uses mechanical disaggregation, ethanol fixation, pepsin treatment, and staining with ethidium bromide. The formalin‐nubtilisin Carlsberg technique resulted in good agreement of ploidy measurements compared to the standard method, a higher number of evaluable histograms, an improved detectability of aneuploid cell populations, and an improved accuracy of the S‐ and G2‐phase analysis, particularly in samples with low proliferation. The method also makes it possible to use long‐term storage and to transport samples by post. © 1993 Wiley‐Liss, Inc." @default.
- W2091219353 created "2016-06-24" @default.
- W2091219353 creator A5063929560 @default.
- W2091219353 creator A5078258693 @default.
- W2091219353 creator A5081632559 @default.
- W2091219353 creator A5085764989 @default.
- W2091219353 date "1993-10-01" @default.
- W2091219353 modified "2023-10-02" @default.
- W2091219353 title "Preparation of cell nuclei from fresh tissues for high-quality DNA flow cytometry" @default.
- W2091219353 cites W1122626307 @default.
- W2091219353 cites W1964582074 @default.
- W2091219353 cites W1969997514 @default.
- W2091219353 cites W1977379434 @default.
- W2091219353 cites W2005208085 @default.
- W2091219353 cites W2014928680 @default.
- W2091219353 cites W2018708063 @default.
- W2091219353 cites W2021901012 @default.
- W2091219353 cites W2048064160 @default.
- W2091219353 cites W2162001480 @default.
- W2091219353 cites W2410010771 @default.
- W2091219353 cites W4249815350 @default.
- W2091219353 doi "https://doi.org/10.1002/cyto.990140712" @default.
- W2091219353 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8243208" @default.
- W2091219353 hasPublicationYear "1993" @default.
- W2091219353 type Work @default.
- W2091219353 sameAs 2091219353 @default.
- W2091219353 citedByCount "67" @default.
- W2091219353 countsByYear W20912193532012 @default.
- W2091219353 countsByYear W20912193532014 @default.
- W2091219353 countsByYear W20912193532017 @default.
- W2091219353 crossrefType "journal-article" @default.
- W2091219353 hasAuthorship W2091219353A5063929560 @default.
- W2091219353 hasAuthorship W2091219353A5078258693 @default.
- W2091219353 hasAuthorship W2091219353A5081632559 @default.
- W2091219353 hasAuthorship W2091219353A5085764989 @default.
- W2091219353 hasBestOaLocation W20912193531 @default.
- W2091219353 hasConcept C1491633281 @default.
- W2091219353 hasConcept C153911025 @default.
- W2091219353 hasConcept C181199279 @default.
- W2091219353 hasConcept C185592680 @default.
- W2091219353 hasConcept C2776708817 @default.
- W2091219353 hasConcept C2776907736 @default.
- W2091219353 hasConcept C2777527632 @default.
- W2091219353 hasConcept C2780339063 @default.
- W2091219353 hasConcept C2780340462 @default.
- W2091219353 hasConcept C43617362 @default.
- W2091219353 hasConcept C54355233 @default.
- W2091219353 hasConcept C552990157 @default.
- W2091219353 hasConcept C553184892 @default.
- W2091219353 hasConcept C55493867 @default.
- W2091219353 hasConcept C74864618 @default.
- W2091219353 hasConcept C7623868 @default.
- W2091219353 hasConcept C86803240 @default.
- W2091219353 hasConceptScore W2091219353C1491633281 @default.
- W2091219353 hasConceptScore W2091219353C153911025 @default.
- W2091219353 hasConceptScore W2091219353C181199279 @default.
- W2091219353 hasConceptScore W2091219353C185592680 @default.
- W2091219353 hasConceptScore W2091219353C2776708817 @default.
- W2091219353 hasConceptScore W2091219353C2776907736 @default.
- W2091219353 hasConceptScore W2091219353C2777527632 @default.
- W2091219353 hasConceptScore W2091219353C2780339063 @default.
- W2091219353 hasConceptScore W2091219353C2780340462 @default.
- W2091219353 hasConceptScore W2091219353C43617362 @default.
- W2091219353 hasConceptScore W2091219353C54355233 @default.
- W2091219353 hasConceptScore W2091219353C552990157 @default.
- W2091219353 hasConceptScore W2091219353C553184892 @default.
- W2091219353 hasConceptScore W2091219353C55493867 @default.
- W2091219353 hasConceptScore W2091219353C74864618 @default.
- W2091219353 hasConceptScore W2091219353C7623868 @default.
- W2091219353 hasConceptScore W2091219353C86803240 @default.
- W2091219353 hasIssue "7" @default.
- W2091219353 hasLocation W20912193531 @default.
- W2091219353 hasLocation W20912193532 @default.
- W2091219353 hasOpenAccess W2091219353 @default.
- W2091219353 hasPrimaryLocation W20912193531 @default.
- W2091219353 hasRelatedWork W1623445113 @default.
- W2091219353 hasRelatedWork W1974173911 @default.
- W2091219353 hasRelatedWork W1982782855 @default.
- W2091219353 hasRelatedWork W1983814878 @default.
- W2091219353 hasRelatedWork W1995181257 @default.
- W2091219353 hasRelatedWork W2015083890 @default.
- W2091219353 hasRelatedWork W2081994183 @default.
- W2091219353 hasRelatedWork W2091219353 @default.
- W2091219353 hasRelatedWork W2146602665 @default.
- W2091219353 hasRelatedWork W2467106685 @default.
- W2091219353 hasVolume "14" @default.
- W2091219353 isParatext "false" @default.
- W2091219353 isRetracted "false" @default.
- W2091219353 magId "2091219353" @default.
- W2091219353 workType "article" @default.