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• W2091228986 abstract "The distribution of Frankia populations in soil is usually studied by plant trapping in combination with genetic characterization. We compared this approach with the evaluation of population distribution by polymerase chain reaction (PCR) and hybridization. Seven populations of Frankia spp. strains, characterized by restriction by HaeIII of the nifD-nifK amplified fragments (nif-HaeIII profiles), were found to have different distribution in a soil column of 60 cm in depth. The nifD-nifK intergenic spacer and the 5'-part of the nifK gene were sequenced for one representative of the seven nif-HaeIII profiles to design specific oligonucleotide probes. The results on the distribution of Frankia populations by PCR amplification on DNA extracted directly from samples from three soil depths (10-20 cm; 30-40 cm; 50-60 cm) and hybridization with specific probes are congruent with the distribution observed by plant trapping.Key words: nif genes, soil, detection, Frankia, PCR, hybridization, plant trapping." @default.
• W2091228986 created "2016-06-24" @default.
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• W2091228986 date "1999-12-18" @default.
• W2091228986 modified "2023-10-17" @default.
• W2091228986 title "Polymerase chain reaction and hybridization on DNA extracted from soil as a tool forFrankiaspp. population distribution studies in soil" @default.
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• W2091228986 doi "https://doi.org/10.1139/b99-065" @default.
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