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• W2091231612 abstract "A fluorescein derivative, sensor 1, was synthesized and characterized. It shows 50 nm bathochromic shifts (from 346 to 400 nm) with a change of solution color and a large increase in the fluorescence intensity upon binding zinc in 1:1 ethanol–hydroxyethyl piperazine ethanesulfonic acid buffer (v/v, pH 7.2) solution. It was demonstrated that changes are not due to the ring opening of the fluorescein spirolactam. The formation of 1–Zn2+ complex or fluorescein dianion adduct at higher pH was further confirmed by mass spectrometry. The fluorescein based molecular probe showed Zn2+ induced absorption and emission changes but does not undergo spirolactam ring opening (reversible) whereas ring opening (irreversible) and hydrolysis take place in alkaline pH region. Confocal microscopy experiments showed that 1 can be used for detection of Zn2+ levels (∼30 μM) within living cells." @default.
• W2091231612 created "2016-06-24" @default.
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• W2091231612 date "2014-10-01" @default.
• W2091231612 modified "2023-10-03" @default.
• W2091231612 title "Fluorescein-based chromo-fluorescent probe for zinc in aqueous solution: Spirolactam ring opened or closed?" @default.
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• W2091231612 doi "https://doi.org/10.1016/j.snb.2014.05.019" @default.
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