FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091250415> ?p ?o ?g. }

• W2091250415 endingPage "182" @default.
• W2091250415 startingPage "171" @default.
• W2091250415 abstract "Parcs/Gpn3 is a putative GTPase that is conserved in eukaryotic cells from yeast to humans, suggesting that it plays a fundamental, but still unknown, cellular function. Suppression of Parcs/Gpn3 expression by RNAi completely blocked cell proliferation in MCF-12A cells and other mammary epithelial cell lines. Unexpectedly, Parcs/Gpn3 knockdown had a more modest effect in the proliferation of the tumorigenic MDA-MB-231 and SK-BR3 cells. RNA polymerase II (RNAP II) co-immunoprecipitated with Parcs/Gpn3. Parcs/Gpn3 depletion caused a reduction in overall RNA synthesis in MCF-12A cells but not in MDA-MB-231 cells, demonstrating a role for Parcs/Gpn3 in transcription, and pointing to a defect in RNA synthesis by RNAP II as the possible cause of halted proliferation. The absence of Parcs/Gpn3 in MCF-12A cells caused a dramatic change in the sub-cellular localization of Rpb1, the largest subunit of RNAP II. As expected, Rpb1 was present only in the nucleus of MCF-12A control cells, whereas in Parcs/Gpn3-depleted MCF-12A cells, Rpb1 was detected exclusively in the cytoplasm. This effect was specific, as histones remained nuclear independently of Parcs/Gpn3. Rpb1 protein levels were markedly increased in Parcs/Gpn3-depleted MCF-12A cells. Interestingly, Rpb1 distribution was only marginally affected after knocking-down Parcs/Gpn3 in MDA-MB-231 cells. In conclusion, we report here, for the first time, that Parcs/Gpn3 plays a critical role in the nuclear accumulation of RNAP II, and we propose that this function explains the relative importance of Parcs/Gpn3 in cell proliferation. Intriguingly, at least some tumorigenic mammary cells have evolved mechanisms that allow them to proliferate in a Parcs/Gpn3-independent manner." @default.
• W2091250415 created "2016-06-24" @default.
• W2091250415 creator A5086907855 @default.
• W2091250415 date "1995-04-01" @default.
• W2091250415 modified "2023-10-10" @default.
• W2091250415 title "Phosphorylation of the C-terminal domain of RNA polymerase II" @default.
• W2091250415 cites W1448454920 @default.
• W2091250415 cites W1484532115 @default.
• W2091250415 cites W1491597935 @default.
• W2091250415 cites W1497435365 @default.
• W2091250415 cites W1512828307 @default.
• W2091250415 cites W1514266791 @default.
• W2091250415 cites W1524975259 @default.
• W2091250415 cites W1525979122 @default.
• W2091250415 cites W1532893184 @default.
• W2091250415 cites W1533728933 @default.
• W2091250415 cites W1538598346 @default.
• W2091250415 cites W1546294341 @default.
• W2091250415 cites W1546324908 @default.
• W2091250415 cites W1550970695 @default.
• W2091250415 cites W1551469893 @default.
• W2091250415 cites W1563156486 @default.
• W2091250415 cites W1577893399 @default.
• W2091250415 cites W1578647589 @default.
• W2091250415 cites W1583104418 @default.
• W2091250415 cites W1594857663 @default.
• W2091250415 cites W1606267994 @default.
• W2091250415 cites W183849683 @default.
• W2091250415 cites W1843768908 @default.
• W2091250415 cites W1894335898 @default.
• W2091250415 cites W1963868441 @default.
• W2091250415 cites W1975948611 @default.
• W2091250415 cites W1994252934 @default.
• W2091250415 cites W1996072788 @default.
• W2091250415 cites W2000889791 @default.
• W2091250415 cites W2002806722 @default.
• W2091250415 cites W2004796688 @default.
• W2091250415 cites W2015599928 @default.
• W2091250415 cites W2018494368 @default.
• W2091250415 cites W2021943181 @default.
• W2091250415 cites W2022517270 @default.
• W2091250415 cites W2030977937 @default.
• W2091250415 cites W2037300780 @default.
• W2091250415 cites W2041101605 @default.
• W2091250415 cites W2042134050 @default.
• W2091250415 cites W2048255483 @default.
• W2091250415 cites W2056277197 @default.
• W2091250415 cites W2056526354 @default.
• W2091250415 cites W2058022253 @default.
• W2091250415 cites W2062195188 @default.
• W2091250415 cites W2068577315 @default.
• W2091250415 cites W2069875752 @default.
• W2091250415 cites W2075263567 @default.
• W2091250415 cites W2075716846 @default.
• W2091250415 cites W2093633115 @default.
• W2091250415 cites W2095215072 @default.
• W2091250415 cites W2110480019 @default.
• W2091250415 cites W2120434953 @default.
• W2091250415 cites W2130709548 @default.
• W2091250415 cites W2134935936 @default.
• W2091250415 cites W2145153782 @default.
• W2091250415 cites W2148668495 @default.
• W2091250415 cites W2152927202 @default.
• W2091250415 cites W2161932414 @default.
• W2091250415 cites W2293975284 @default.
• W2091250415 cites W236095070 @default.
• W2091250415 cites W4240862133 @default.
• W2091250415 cites W4244866225 @default.
• W2091250415 cites W4378744478 @default.
• W2091250415 doi "https://doi.org/10.1016/0167-4781(94)00233-s" @default.
• W2091250415 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7711060" @default.
• W2091250415 hasPublicationYear "1995" @default.
• W2091250415 type Work @default.
• W2091250415 sameAs 2091250415 @default.
• W2091250415 citedByCount "123" @default.
• W2091250415 countsByYear W20912504152012 @default.
• W2091250415 countsByYear W20912504152013 @default.
• W2091250415 countsByYear W20912504152015 @default.
• W2091250415 countsByYear W20912504152017 @default.
• W2091250415 countsByYear W20912504152020 @default.
• W2091250415 countsByYear W20912504152021 @default.
• W2091250415 countsByYear W20912504152023 @default.
• W2091250415 crossrefType "journal-article" @default.
• W2091250415 hasAuthorship W2091250415A5086907855 @default.
• W2091250415 hasConcept C101762097 @default.
• W2091250415 hasConcept C104317684 @default.
• W2091250415 hasConcept C138885662 @default.
• W2091250415 hasConcept C150194340 @default.
• W2091250415 hasConcept C153911025 @default.
• W2091250415 hasConcept C173396325 @default.
• W2091250415 hasConcept C179926584 @default.
• W2091250415 hasConcept C190062978 @default.
• W2091250415 hasConcept C41895202 @default.
• W2091250415 hasConcept C54355233 @default.
• W2091250415 hasConcept C64350747 @default.
• W2091250415 hasConcept C67705224 @default.
• W2091250415 hasConcept C81885089 @default.
• W2091250415 hasConcept C86803240 @default.

• next