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- W2091266772 abstract "The effect of guanidine chloride on the activity of bovine heart lactate dehydrogenase transferred to a system that was made with toluene, phospholipids, Triton X-100 and 3.8% water (v/v) was studied. The activity of the enzyme in the latter system was about 30 times lower than in standard water mixtures. In the low water system, 1.5 and 2.0 M guanidine chloride increased the activity by approximately 20 times. These concentrations of guanidine chloride caused complete inactivation of the enzyme in conventional water systems. The activating effect of the denaturant was independent of enzyme concentration. It is suggested that the increase in activity produced by guanidine chloride was due to a facilitation of the protein-solvent interactions that operate in a catalytic cycle." @default.
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- W2091266772 date "1990-10-01" @default.
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- W2091266772 title "High concentrations of guanidine chloride activate lactate dehydrogenase in low water media" @default.
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- W2091266772 doi "https://doi.org/10.1016/0006-291x(90)90750-h" @default.
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