Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091267909> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2091267909 endingPage "252" @default.
- W2091267909 startingPage "242" @default.
- W2091267909 abstract "Purification of α-fetoprotein from mouse hepatoma BW7756 extracts was performed using ammonium sulfate precipitation, gel filtration, ion-exchange chromatography and isoelectric focusing. These procedures produced a 5.6% yield of α-fetoprotein with 96% purity. Polyacrylamide slab gel electrophoresis, extended agarose electrophoresis and immunoelectrophoresis demonstrated that mouse hepatoma α-fetoprotein migrated at pH 8.6 as a rapid α1, or postalbumin globulin. Crossed antibody electrophoresis of the agarose zone containing α-fetoprotein failed to demonstrate microheterogeneity. Molecular weight analysis of the mouse hepatoma α-fetoprotein on a calibrated Sephadex G-200 column yielded a value of 72 000–73 000 for the native protein. Sodium dodecyl sulfate gel electrophoresis subsequently demonstrated a single polypeptide chain with a molecular weight of 72 000. Amino acid analysis showed the α-fetoprotein to be an acidic protein dominated by hydrophobic residues. The total carbohydrate content was 5.5% and 3 mol of sialic acid were detected per mol of α-fetoprotein. Although neutral sugars were the principal class present, galactosamine was the most abundant single sugar detected." @default.
- W2091267909 created "2016-06-24" @default.
- W2091267909 creator A5050060233 @default.
- W2091267909 creator A5082154683 @default.
- W2091267909 date "1977-03-01" @default.
- W2091267909 modified "2023-09-26" @default.
- W2091267909 title "Isolation and characterization of α-fetoprotein from the mouse hepatoma bw7756" @default.
- W2091267909 cites W1524026612 @default.
- W2091267909 cites W1600918029 @default.
- W2091267909 cites W1625660792 @default.
- W2091267909 cites W1775749144 @default.
- W2091267909 cites W1829494802 @default.
- W2091267909 cites W1971384542 @default.
- W2091267909 cites W1975023463 @default.
- W2091267909 cites W1976030942 @default.
- W2091267909 cites W1982415717 @default.
- W2091267909 cites W2002983094 @default.
- W2091267909 cites W2010597861 @default.
- W2091267909 cites W2017470279 @default.
- W2091267909 cites W2017731369 @default.
- W2091267909 cites W2018377262 @default.
- W2091267909 cites W2036839010 @default.
- W2091267909 cites W2067674360 @default.
- W2091267909 cites W2077263010 @default.
- W2091267909 cites W2078740870 @default.
- W2091267909 cites W2083733119 @default.
- W2091267909 cites W2111301002 @default.
- W2091267909 cites W2217931786 @default.
- W2091267909 cites W2403353360 @default.
- W2091267909 cites W4302345199 @default.
- W2091267909 doi "https://doi.org/10.1016/0005-2795(77)90060-5" @default.
- W2091267909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/66068" @default.
- W2091267909 hasPublicationYear "1977" @default.
- W2091267909 type Work @default.
- W2091267909 sameAs 2091267909 @default.
- W2091267909 citedByCount "16" @default.
- W2091267909 crossrefType "journal-article" @default.
- W2091267909 hasAuthorship W2091267909A5050060233 @default.
- W2091267909 hasAuthorship W2091267909A5082154683 @default.
- W2091267909 hasConcept C101660505 @default.
- W2091267909 hasConcept C116084860 @default.
- W2091267909 hasConcept C163994747 @default.
- W2091267909 hasConcept C181199279 @default.
- W2091267909 hasConcept C185592680 @default.
- W2091267909 hasConcept C193363816 @default.
- W2091267909 hasConcept C2778476535 @default.
- W2091267909 hasConcept C2778913249 @default.
- W2091267909 hasConcept C2780115458 @default.
- W2091267909 hasConcept C43617362 @default.
- W2091267909 hasConcept C50476208 @default.
- W2091267909 hasConcept C55493867 @default.
- W2091267909 hasConceptScore W2091267909C101660505 @default.
- W2091267909 hasConceptScore W2091267909C116084860 @default.
- W2091267909 hasConceptScore W2091267909C163994747 @default.
- W2091267909 hasConceptScore W2091267909C181199279 @default.
- W2091267909 hasConceptScore W2091267909C185592680 @default.
- W2091267909 hasConceptScore W2091267909C193363816 @default.
- W2091267909 hasConceptScore W2091267909C2778476535 @default.
- W2091267909 hasConceptScore W2091267909C2778913249 @default.
- W2091267909 hasConceptScore W2091267909C2780115458 @default.
- W2091267909 hasConceptScore W2091267909C43617362 @default.
- W2091267909 hasConceptScore W2091267909C50476208 @default.
- W2091267909 hasConceptScore W2091267909C55493867 @default.
- W2091267909 hasIssue "1" @default.
- W2091267909 hasLocation W20912679091 @default.
- W2091267909 hasLocation W20912679092 @default.
- W2091267909 hasOpenAccess W2091267909 @default.
- W2091267909 hasPrimaryLocation W20912679091 @default.
- W2091267909 hasRelatedWork W1894899954 @default.
- W2091267909 hasRelatedWork W1924602487 @default.
- W2091267909 hasRelatedWork W1954232397 @default.
- W2091267909 hasRelatedWork W1988452686 @default.
- W2091267909 hasRelatedWork W2001321907 @default.
- W2091267909 hasRelatedWork W2019405391 @default.
- W2091267909 hasRelatedWork W2022994535 @default.
- W2091267909 hasRelatedWork W2037000062 @default.
- W2091267909 hasRelatedWork W2054862544 @default.
- W2091267909 hasRelatedWork W2117141996 @default.
- W2091267909 hasVolume "491" @default.
- W2091267909 isParatext "false" @default.
- W2091267909 isRetracted "false" @default.
- W2091267909 magId "2091267909" @default.
- W2091267909 workType "article" @default.