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• W2091268676 endingPage "4912" @default.
• W2091268676 startingPage "4905" @default.
• W2091268676 abstract "There is no ideal material for craniofacial bone repair at present. The aim of this study was to test the biocompatibility of polycaprolactone (PCL) synthesised by a novel method allowing control of molecular weight and degradation rate, with regard to it being used as matrix for a biodegradable composite for craniofacial bone repair. Human primary craniofacial cells were used, isolated from paediatric skull after surgery. Cell responses were analysed using various assays and antibody staining. Cells attached and spread on the PCL in a similar manner to the Thermanox controls as shown by phalloidin staining of F-actin. Cells maintained the osteoblast phenotype as demonstrated by alkaline phosphatase assay and antibody staining throughout the time points studied, up to 28 days. Cells proliferated on the PCL as shown by a DNA assay. Collagen-1 staining showed extensive production of a collagen-1 containing extracellular matrix, which was also shown to be mineralised by alizarin red staining. Short-term (up to 48 h) attachment studies and long-term (up to 28 days) expression of markers of the osteoblast phenotype have been demonstrated on the PCL. This new method of synthesising PCL shows biocompatibility characteristics that give it potential to be used for craniofacial bone repair." @default.
• W2091268676 created "2016-06-24" @default.
• W2091268676 creator A5025547174 @default.
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• W2091268676 date "2003-12-01" @default.
• W2091268676 modified "2023-10-16" @default.
• W2091268676 title "Craniofacial osteoblast responses to polycaprolactone produced using a novel boron polymerisation technique and potassium fluoride post-treatment" @default.
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• W2091268676 doi "https://doi.org/10.1016/s0142-9612(03)00409-5" @default.
• W2091268676 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14559003" @default.
• W2091268676 hasPublicationYear "2003" @default.
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