FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091273235> ?p ?o ?g. }

• W2091273235 endingPage "1306" @default.
• W2091273235 startingPage "1297" @default.
• W2091273235 abstract "We have reported that numerous tartrate-resistant acid phosphatase-positive osteoclast-like multinucleated cells (TRAP+ MNCs) are formed when mouse osteoblastic cells and spleen cells are cocultured in the presence of 1α25-dihydroxyvitamin D3 [1α,25-(OH)2D3] (Endocrinology 123:2600, 1988). In this study, we prepared a TRAP+ MNC population using a modified coculture system and examined its osteoclastic properties. TRAP+ MNCs were formed in cocultures of mouse osteoblastic cells and marrow cells on 10 cm collagen gelcoated dishes. The TRAP+ MNC population was prepared by treating the dishes with 0.2% bacterial collagenase followed by density gradient centrifugation. The yield of TRAP+ MNCs was 20,000–40,000 cells per dish, much higher than that of osteoclasts (OCLs) isolated from neonatal rat bones (∼ 1000 cells per head). The purity of TRAP+ MNCs was 5.6 ± 0.6% in cell number and about 30% in the number of nuclei. The recovery of TRAP+ MNCs after density gradient centrifugation was 30–40%. Acid production by MNCs was demonstrated by vital staining with acridine orange. Numerous resorption pits were formed when the MNC population was cultured for 48 h on bone slices. Autoradiography using [125I]salmon calcitonin (CT) showed abundant CT binding in most TRAP+ MNCs. Saturation analysis of [125I]salmon CT indicated a dissociation constant Kd for TRAP+ MNCs of 8.9 ± 0.7 × 1010 M and 16.5 ± 1.5 ± 106 binding sites per cell. These results were similar to the value (3.5 × 10−10 M) and the number of binding sites (3.3 × 106 per cell) in isolated rat OCLs. Displacement curves for [125I]salmon CT with unlabeled salmon and human CT were similar in MNC and OCL preparations. Salmon and human CT increased cAMP production (maximal response: salmon CT at 10−10 M, human CT at 10−8 M; ED50: salmon CT, 2.2 × 10−11 M, human CT, 1.3 × 10−9 M) in the MNC preparation. These results indicate that a large number of mouse TRAP+ MNCs possessing OCL characteristics can be easily prepared from in vitro cultures. This procedure will facilitate examination of mammalian OCL functions." @default.
• W2091273235 created "2016-06-24" @default.
• W2091273235 creator A5002464903 @default.
• W2091273235 creator A5004859597 @default.
• W2091273235 creator A5034320377 @default.
• W2091273235 creator A5046260853 @default.
• W2091273235 creator A5046699754 @default.
• W2091273235 creator A5049808190 @default.
• W2091273235 creator A5054636847 @default.
• W2091273235 creator A5055584432 @default.
• W2091273235 creator A5055741008 @default.
• W2091273235 date "2009-12-03" @default.
• W2091273235 modified "2023-10-12" @default.
• W2091273235 title "Preparation and characterization of a mouse osteoclast-like multinucleated cell population" @default.
• W2091273235 cites W1970121439 @default.
• W2091273235 cites W1970256789 @default.
• W2091273235 cites W1974467083 @default.
• W2091273235 cites W1977553486 @default.
• W2091273235 cites W1979713373 @default.
• W2091273235 cites W1989864418 @default.
• W2091273235 cites W1999826236 @default.
• W2091273235 cites W1999968337 @default.
• W2091273235 cites W2000170431 @default.
• W2091273235 cites W2009672068 @default.
• W2091273235 cites W2018634464 @default.
• W2091273235 cites W2021420430 @default.
• W2091273235 cites W2030261305 @default.
• W2091273235 cites W2043651158 @default.
• W2091273235 cites W2044482752 @default.
• W2091273235 cites W2046662935 @default.
• W2091273235 cites W2046758804 @default.
• W2091273235 cites W2075356469 @default.
• W2091273235 cites W2084376887 @default.
• W2091273235 cites W2091478316 @default.
• W2091273235 cites W2093441137 @default.
• W2091273235 cites W2102285032 @default.
• W2091273235 cites W2148006057 @default.
• W2091273235 cites W2170292249 @default.
• W2091273235 cites W4254656286 @default.
• W2091273235 doi "https://doi.org/10.1002/jbmr.5650071109" @default.
• W2091273235 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1281605" @default.
• W2091273235 hasPublicationYear "2009" @default.
• W2091273235 type Work @default.
• W2091273235 sameAs 2091273235 @default.
• W2091273235 citedByCount "150" @default.
• W2091273235 countsByYear W20912732352012 @default.
• W2091273235 countsByYear W20912732352013 @default.
• W2091273235 countsByYear W20912732352015 @default.
• W2091273235 countsByYear W20912732352016 @default.
• W2091273235 countsByYear W20912732352018 @default.
• W2091273235 countsByYear W20912732352019 @default.
• W2091273235 countsByYear W20912732352020 @default.
• W2091273235 countsByYear W20912732352022 @default.
• W2091273235 countsByYear W20912732352023 @default.
• W2091273235 crossrefType "journal-article" @default.
• W2091273235 hasAuthorship W2091273235A5002464903 @default.
• W2091273235 hasAuthorship W2091273235A5004859597 @default.
• W2091273235 hasAuthorship W2091273235A5034320377 @default.
• W2091273235 hasAuthorship W2091273235A5046260853 @default.
• W2091273235 hasAuthorship W2091273235A5046699754 @default.
• W2091273235 hasAuthorship W2091273235A5049808190 @default.
• W2091273235 hasAuthorship W2091273235A5054636847 @default.
• W2091273235 hasAuthorship W2091273235A5055584432 @default.
• W2091273235 hasAuthorship W2091273235A5055741008 @default.
• W2091273235 hasConcept C126322002 @default.
• W2091273235 hasConcept C134018914 @default.
• W2091273235 hasConcept C136403682 @default.
• W2091273235 hasConcept C153911025 @default.
• W2091273235 hasConcept C180675623 @default.
• W2091273235 hasConcept C185592680 @default.
• W2091273235 hasConcept C202751555 @default.
• W2091273235 hasConcept C203014093 @default.
• W2091273235 hasConcept C2776033226 @default.
• W2091273235 hasConcept C2780007613 @default.
• W2091273235 hasConcept C2908647359 @default.
• W2091273235 hasConcept C55493867 @default.
• W2091273235 hasConcept C673006 @default.
• W2091273235 hasConcept C71924100 @default.
• W2091273235 hasConcept C86803240 @default.
• W2091273235 hasConcept C95444343 @default.
• W2091273235 hasConcept C99454951 @default.
• W2091273235 hasConceptScore W2091273235C126322002 @default.
• W2091273235 hasConceptScore W2091273235C134018914 @default.
• W2091273235 hasConceptScore W2091273235C136403682 @default.
• W2091273235 hasConceptScore W2091273235C153911025 @default.
• W2091273235 hasConceptScore W2091273235C180675623 @default.
• W2091273235 hasConceptScore W2091273235C185592680 @default.
• W2091273235 hasConceptScore W2091273235C202751555 @default.
• W2091273235 hasConceptScore W2091273235C203014093 @default.
• W2091273235 hasConceptScore W2091273235C2776033226 @default.
• W2091273235 hasConceptScore W2091273235C2780007613 @default.
• W2091273235 hasConceptScore W2091273235C2908647359 @default.
• W2091273235 hasConceptScore W2091273235C55493867 @default.
• W2091273235 hasConceptScore W2091273235C673006 @default.
• W2091273235 hasConceptScore W2091273235C71924100 @default.
• W2091273235 hasConceptScore W2091273235C86803240 @default.
• W2091273235 hasConceptScore W2091273235C95444343 @default.
• W2091273235 hasConceptScore W2091273235C99454951 @default.

• next