Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091277893> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2091277893 abstract "Two membrane-bound enzymes of Proteus mirabilis with the dual functions of peptidoglycan DD-carboxypeptidase and transpeptidase (named DD-carboxypeptidase/transpeptidase H and L) were isolated and purified by selective solubilization with the nonionic detergent Genapol X-100, affinity chromatography on matrix-bound ampicillin, and preparative isoelectric focusing in the presence of detergent. Purified enzymes H and L were, respectively, penicillin-binding proteins 4 and 5 among seven major penicillin-binding proteins present in P. mirabilis. The enzymes differed in the following properties. Enzyme H had an Mr of 49,000; isoelectric point at pH 8.2; high sensitivity to benzylpenicillin and permanent inactivation because of high stability of the enzyme-antibiotic complex EI* (half-life 300 min); fragmentation of benzylpenicillin with formation of phenylacetylglycine during the slow decay of EI*; it functioned as an endopeptidase on peptide-crosslinked side chains of peptidoglycan. Enzyme L had an Mr of 43 000; isoelectric point at pH 5.9; low sensitivity to benzylpenicillin and low stability of EI* (half-life 7.2 min) with rapid recovery of enzyme activity; no function as an endopeptidase. The properties of enzyme L were identical with those of the single active DD-carboxypeptidase found previously in the spheroplast L-form of P. mirabilis grown in the presence of benzylpenicillin. We conclude that the partial penicillin resistance of P. mirabilis, with growth as L-form and synthesis of peptide-crosslinked peptidoglycan, depends on the continuing fuction of enzyme L as a DD-carboxypeptidase and transpeptidase in the presence of the antibiotic." @default.
- W2091277893 created "2016-06-24" @default.
- W2091277893 creator A5046640842 @default.
- W2091277893 creator A5064363611 @default.
- W2091277893 date "1980-04-01" @default.
- W2091277893 modified "2023-09-26" @default.
- W2091277893 title "Purification of Two DD-Carboxypeptidases/Transpeptidases with Different Penicillin Sensitivities from Proteus mirabilis" @default.
- W2091277893 cites W1480318552 @default.
- W2091277893 cites W1550519918 @default.
- W2091277893 cites W1625660792 @default.
- W2091277893 cites W1678903690 @default.
- W2091277893 cites W1936422283 @default.
- W2091277893 cites W1968526218 @default.
- W2091277893 cites W1969464489 @default.
- W2091277893 cites W1980981893 @default.
- W2091277893 cites W1981436667 @default.
- W2091277893 cites W1992006277 @default.
- W2091277893 cites W1995464211 @default.
- W2091277893 cites W2002177586 @default.
- W2091277893 cites W2004562533 @default.
- W2091277893 cites W2015956634 @default.
- W2091277893 cites W2017583938 @default.
- W2091277893 cites W2023038885 @default.
- W2091277893 cites W2027393724 @default.
- W2091277893 cites W2031771695 @default.
- W2091277893 cites W2033841549 @default.
- W2091277893 cites W2035131577 @default.
- W2091277893 cites W2052140557 @default.
- W2091277893 cites W2080751038 @default.
- W2091277893 cites W2088283313 @default.
- W2091277893 cites W2089022807 @default.
- W2091277893 cites W2092848100 @default.
- W2091277893 cites W2100837269 @default.
- W2091277893 cites W2120886706 @default.
- W2091277893 cites W2122329462 @default.
- W2091277893 cites W2167973356 @default.
- W2091277893 cites W2342374885 @default.
- W2091277893 cites W4248412469 @default.
- W2091277893 doi "https://doi.org/10.1111/j.1432-1033.1980.tb04509.x" @default.
- W2091277893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7379792" @default.
- W2091277893 hasPublicationYear "1980" @default.
- W2091277893 type Work @default.
- W2091277893 sameAs 2091277893 @default.
- W2091277893 citedByCount "15" @default.
- W2091277893 countsByYear W20912778932014 @default.
- W2091277893 crossrefType "journal-article" @default.
- W2091277893 hasAuthorship W2091277893A5046640842 @default.
- W2091277893 hasAuthorship W2091277893A5064363611 @default.
- W2091277893 hasBestOaLocation W20912778931 @default.
- W2091277893 hasConcept C104317684 @default.
- W2091277893 hasConcept C108477463 @default.
- W2091277893 hasConcept C174802600 @default.
- W2091277893 hasConcept C181199279 @default.
- W2091277893 hasConcept C185592680 @default.
- W2091277893 hasConcept C2776341680 @default.
- W2091277893 hasConcept C2776634448 @default.
- W2091277893 hasConcept C2777774944 @default.
- W2091277893 hasConcept C2778039505 @default.
- W2091277893 hasConcept C43617362 @default.
- W2091277893 hasConcept C501593827 @default.
- W2091277893 hasConcept C50476208 @default.
- W2091277893 hasConcept C547475151 @default.
- W2091277893 hasConcept C55493867 @default.
- W2091277893 hasConcept C96294017 @default.
- W2091277893 hasConceptScore W2091277893C104317684 @default.
- W2091277893 hasConceptScore W2091277893C108477463 @default.
- W2091277893 hasConceptScore W2091277893C174802600 @default.
- W2091277893 hasConceptScore W2091277893C181199279 @default.
- W2091277893 hasConceptScore W2091277893C185592680 @default.
- W2091277893 hasConceptScore W2091277893C2776341680 @default.
- W2091277893 hasConceptScore W2091277893C2776634448 @default.
- W2091277893 hasConceptScore W2091277893C2777774944 @default.
- W2091277893 hasConceptScore W2091277893C2778039505 @default.
- W2091277893 hasConceptScore W2091277893C43617362 @default.
- W2091277893 hasConceptScore W2091277893C501593827 @default.
- W2091277893 hasConceptScore W2091277893C50476208 @default.
- W2091277893 hasConceptScore W2091277893C547475151 @default.
- W2091277893 hasConceptScore W2091277893C55493867 @default.
- W2091277893 hasConceptScore W2091277893C96294017 @default.
- W2091277893 hasLocation W20912778931 @default.
- W2091277893 hasLocation W20912778932 @default.
- W2091277893 hasOpenAccess W2091277893 @default.
- W2091277893 hasPrimaryLocation W20912778931 @default.
- W2091277893 hasRelatedWork W153595000 @default.
- W2091277893 hasRelatedWork W1566747358 @default.
- W2091277893 hasRelatedWork W1981436667 @default.
- W2091277893 hasRelatedWork W1986924706 @default.
- W2091277893 hasRelatedWork W2004562533 @default.
- W2091277893 hasRelatedWork W2091277893 @default.
- W2091277893 hasRelatedWork W2104968341 @default.
- W2091277893 hasRelatedWork W2130730370 @default.
- W2091277893 hasRelatedWork W2166598973 @default.
- W2091277893 hasRelatedWork W2084674474 @default.
- W2091277893 isParatext "false" @default.
- W2091277893 isRetracted "false" @default.
- W2091277893 magId "2091277893" @default.
- W2091277893 workType "article" @default.