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• W2091279558 abstract "Objectives: To develop an immunoassay for osteopontin (OPN), a secreted phosphoglycoprotein that is implicated in a number of human diseases, and establish basal plasma OPN levels in healthy women. Design and Methods: An antigen-capture ELISA was developed to quantify OPN in plasma using a combination of mouse monoclonal and rabbit polyclonal antibodies. Basal OPN levels were determined in blood plasma of 21 pre- and 14 postmenopausal women obtained at 7-day intervals over a 4-week period. Results: A group of 35 healthy women had a median OPN level of 31 μg/L (range = 14–64 μg/L;). Comparison between pre- and post-menopausal women showed that their 4-week average OPN levels did not differ significantly (p > 0.16, Mann-Whitney test), and that levels in each premenopausal individual remained constant during the menstrual cycle, unaffected by cyclical levels of leuteinizing hormone and progesterone. Conclusion: Systematic quantification of plasma OPN can now be done by ELISA, which was used to establish basal plasma OPN levels in a group of healthy women. Levels in pre- and postmenopausal women appeared relatively stable over a 4-week period." @default.
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• W2091279558 date "1996-06-01" @default.
• W2091279558 modified "2023-10-16" @default.
• W2091279558 title "Quantification of osteopontin in human plasma with an ELISA: Basal levels in pre- and postmenopausal women" @default.
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• W2091279558 doi "https://doi.org/10.1016/0009-9120(96)84728-a" @default.
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