FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091280495> ?p ?o ?g. }

• W2091280495 abstract "We reported recently that roscovitine (ROSC), a selective cyclin-dependent kinase (CDK) inhibitor, arrests human MCF-7 breast cancer cells in G(2) phase of the cell cycle, and concomitantly induces apoptosis. Human MCF-7 breast cancer cells are known to express elevated levels of c-Ha-Ras protein. To achieve full biological activity, de novo synthesized c-Ha-Ras protein has to be farnesylated and after further processing it needs to be attached to the plasma membrane. Therefore, we decided to prove whether prevention of protein farnesylation would sensitize MCF-7 cells to the action of ROSC. MCF-7 cells were treated with 1-40 microM ROSC alone, or in combination with L-744,832, an inhibitor of farnesyl protein transferase (FTPase). To measure the impact on the proliferation of the cells, we used the CellTiterGlo viability assay and FACS analysis was employed to quantify the DNA-content of the single cells. The amount and phosphorylation status of relevant proteins after lysis of MCF-7 cells was assessed on Western blots using (phospho)-specific antibodies. The combined treatment with L-744,832 and ROSC for 24 h, markedly reduced the number of viable MCF-7 cells, primarily, by re-enforcing the cell cycle arrest. Interestingly, the potentiation of the ROSC-mediated inhibition of cell proliferation became evident during the 48 h post-incubation period in presence of the FPTase inhibitor. Inhibition of FPTase in ROSC-treated cells reduced the number of viable cells by approximately 30%. Evidently, the combined treatment sensitizes MCF-7 cells to the action of ROSC, thereby allowing to reduce the dose of the drug and to minimize side effects." @default.
• W2091280495 created "2016-06-24" @default.
• W2091280495 creator A5008317857 @default.
• W2091280495 creator A5049681995 @default.
• W2091280495 creator A5057759899 @default.
• W2091280495 date "2007-01-01" @default.
• W2091280495 modified "2023-09-25" @default.
• W2091280495 title "Inhibition of farnesyl protein transferase sensitizes human MCF-7 breast cancer cells to roscovitine-mediated cell cycle arrest" @default.
• W2091280495 cites W1560077201 @default.
• W2091280495 cites W1944970393 @default.
• W2091280495 cites W1965260678 @default.
• W2091280495 cites W1966211363 @default.
• W2091280495 cites W1971140803 @default.
• W2091280495 cites W1972024414 @default.
• W2091280495 cites W1974624332 @default.
• W2091280495 cites W1977379434 @default.
• W2091280495 cites W1978465495 @default.
• W2091280495 cites W1984606443 @default.
• W2091280495 cites W1991287545 @default.
• W2091280495 cites W1997388033 @default.
• W2091280495 cites W2009234416 @default.
• W2091280495 cites W2011912935 @default.
• W2091280495 cites W2015276971 @default.
• W2091280495 cites W2021381932 @default.
• W2091280495 cites W2025476423 @default.
• W2091280495 cites W2025963365 @default.
• W2091280495 cites W2026834760 @default.
• W2091280495 cites W2032323614 @default.
• W2091280495 cites W2038407368 @default.
• W2091280495 cites W2045000096 @default.
• W2091280495 cites W2045563337 @default.
• W2091280495 cites W2060734153 @default.
• W2091280495 cites W2062120857 @default.
• W2091280495 cites W2065939569 @default.
• W2091280495 cites W2070732456 @default.
• W2091280495 cites W2072124651 @default.
• W2091280495 cites W2075082022 @default.
• W2091280495 cites W2080266051 @default.
• W2091280495 cites W2083280703 @default.
• W2091280495 cites W2093481279 @default.
• W2091280495 cites W2099289675 @default.
• W2091280495 cites W2121619780 @default.
• W2091280495 cites W2126439920 @default.
• W2091280495 cites W2135129680 @default.
• W2091280495 cites W2135713539 @default.
• W2091280495 cites W2144535642 @default.
• W2091280495 cites W2145978772 @default.
• W2091280495 cites W2182253964 @default.
• W2091280495 cites W2316434439 @default.
• W2091280495 cites W2317786914 @default.
• W2091280495 cites W2319148198 @default.
• W2091280495 cites W2329304300 @default.
• W2091280495 cites W2531432194 @default.
• W2091280495 doi "https://doi.org/10.1002/jcb.21325" @default.
• W2091280495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17415788" @default.
• W2091280495 hasPublicationYear "2007" @default.
• W2091280495 type Work @default.
• W2091280495 sameAs 2091280495 @default.
• W2091280495 citedByCount "6" @default.
• W2091280495 countsByYear W20912804952012 @default.
• W2091280495 countsByYear W20912804952014 @default.
• W2091280495 crossrefType "journal-article" @default.
• W2091280495 hasAuthorship W2091280495A5008317857 @default.
• W2091280495 hasAuthorship W2091280495A5049681995 @default.
• W2091280495 hasAuthorship W2091280495A5057759899 @default.
• W2091280495 hasConcept C105696609 @default.
• W2091280495 hasConcept C109541995 @default.
• W2091280495 hasConcept C121608353 @default.
• W2091280495 hasConcept C124320809 @default.
• W2091280495 hasConcept C153911025 @default.
• W2091280495 hasConcept C184235292 @default.
• W2091280495 hasConcept C190283241 @default.
• W2091280495 hasConcept C2778298596 @default.
• W2091280495 hasConcept C29537977 @default.
• W2091280495 hasConcept C2994423619 @default.
• W2091280495 hasConcept C502942594 @default.
• W2091280495 hasConcept C53227056 @default.
• W2091280495 hasConcept C54355233 @default.
• W2091280495 hasConcept C55493867 @default.
• W2091280495 hasConcept C62112901 @default.
• W2091280495 hasConcept C86803240 @default.
• W2091280495 hasConcept C95444343 @default.
• W2091280495 hasConcept C96232424 @default.
• W2091280495 hasConcept C98274493 @default.
• W2091280495 hasConceptScore W2091280495C105696609 @default.
• W2091280495 hasConceptScore W2091280495C109541995 @default.
• W2091280495 hasConceptScore W2091280495C121608353 @default.
• W2091280495 hasConceptScore W2091280495C124320809 @default.
• W2091280495 hasConceptScore W2091280495C153911025 @default.
• W2091280495 hasConceptScore W2091280495C184235292 @default.
• W2091280495 hasConceptScore W2091280495C190283241 @default.
• W2091280495 hasConceptScore W2091280495C2778298596 @default.
• W2091280495 hasConceptScore W2091280495C29537977 @default.
• W2091280495 hasConceptScore W2091280495C2994423619 @default.
• W2091280495 hasConceptScore W2091280495C502942594 @default.
• W2091280495 hasConceptScore W2091280495C53227056 @default.
• W2091280495 hasConceptScore W2091280495C54355233 @default.
• W2091280495 hasConceptScore W2091280495C55493867 @default.
• W2091280495 hasConceptScore W2091280495C62112901 @default.
• W2091280495 hasConceptScore W2091280495C86803240 @default.

• next