Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091293351> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2091293351 endingPage "13932" @default.
- W2091293351 startingPage "13925" @default.
- W2091293351 abstract "We have previously demonstrated that the alpha isoform of Ca(2+)/calmodulin-dependent protein kinase kinase (CaM-KKalpha) is strictly regulated by an autoinhibitory mechanism and activated by the binding of Ca(2+)/CaM [Tokumitsu, H., Muramatsu, M., Ikura, M., and Kobayashi, R. (2000) J. Biol. Chem. 275, 20090-20095]. In this study, we find that rat brain extract contains Ca(2+)/CaM-independent CaM-KK activity. This result is consistent with an enhanced Ca(2+)/CaM-independent activity (60-70% of total activity) observed with the recombinant CaM-KKbeta isoform. By using various truncation mutants of CaM-KKbeta, we have identified a region of 23 amino acids (residues 129-151) located at the N-terminus of the catalytic domain as an important regulatory element of the autonomous activity. A CaM-KKbeta deletion mutant of this domain shows a significant increase of Ca(2+)/CaM dependency for the CaM-KK activity as well as for the autophosphorylation activity. The activities of CaM-KKalpha and CaM-KKbeta chimera, in which autoinhibitory sequences were replaced by each other, were completely dependent on Ca(2+)/CaM, suggesting that the autoinhibitory regions of CaM-KKalpha and CaM-KKbeta are functional. These results establish for the first time that residues 129-151 of CaM-KKbeta participate in the release of the autoinhibitory domain from its catalytic core, resulting in generation of autonomous activity." @default.
- W2091293351 created "2016-06-24" @default.
- W2091293351 creator A5028305352 @default.
- W2091293351 creator A5033830692 @default.
- W2091293351 creator A5060519521 @default.
- W2091293351 creator A5078658497 @default.
- W2091293351 date "2001-10-27" @default.
- W2091293351 modified "2023-09-30" @default.
- W2091293351 title "Differential Regulatory Mechanism of Ca<sup>2+</sup>/Calmodulin-Dependent Protein Kinase Kinase Isoforms" @default.
- W2091293351 cites W1483911606 @default.
- W2091293351 cites W1484384039 @default.
- W2091293351 cites W1500240510 @default.
- W2091293351 cites W1967305385 @default.
- W2091293351 cites W1970808596 @default.
- W2091293351 cites W1985239587 @default.
- W2091293351 cites W1989260964 @default.
- W2091293351 cites W1989734556 @default.
- W2091293351 cites W2000423709 @default.
- W2091293351 cites W2045381482 @default.
- W2091293351 cites W2061412811 @default.
- W2091293351 cites W2066636450 @default.
- W2091293351 cites W2071014862 @default.
- W2091293351 cites W2074112908 @default.
- W2091293351 cites W2088033659 @default.
- W2091293351 cites W2149785766 @default.
- W2091293351 doi "https://doi.org/10.1021/bi010863k" @default.
- W2091293351 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11705382" @default.
- W2091293351 hasPublicationYear "2001" @default.
- W2091293351 type Work @default.
- W2091293351 sameAs 2091293351 @default.
- W2091293351 citedByCount "76" @default.
- W2091293351 countsByYear W20912933512012 @default.
- W2091293351 countsByYear W20912933512013 @default.
- W2091293351 countsByYear W20912933512014 @default.
- W2091293351 countsByYear W20912933512015 @default.
- W2091293351 countsByYear W20912933512016 @default.
- W2091293351 countsByYear W20912933512017 @default.
- W2091293351 countsByYear W20912933512018 @default.
- W2091293351 countsByYear W20912933512019 @default.
- W2091293351 countsByYear W20912933512020 @default.
- W2091293351 countsByYear W20912933512021 @default.
- W2091293351 countsByYear W20912933512022 @default.
- W2091293351 countsByYear W20912933512023 @default.
- W2091293351 crossrefType "journal-article" @default.
- W2091293351 hasAuthorship W2091293351A5028305352 @default.
- W2091293351 hasAuthorship W2091293351A5033830692 @default.
- W2091293351 hasAuthorship W2091293351A5060519521 @default.
- W2091293351 hasAuthorship W2091293351A5078658497 @default.
- W2091293351 hasConcept C104317684 @default.
- W2091293351 hasConcept C143065580 @default.
- W2091293351 hasConcept C150109051 @default.
- W2091293351 hasConcept C181199279 @default.
- W2091293351 hasConcept C184235292 @default.
- W2091293351 hasConcept C185592680 @default.
- W2091293351 hasConcept C29688787 @default.
- W2091293351 hasConcept C32619005 @default.
- W2091293351 hasConcept C53345823 @default.
- W2091293351 hasConcept C55493867 @default.
- W2091293351 hasConcept C86803240 @default.
- W2091293351 hasConcept C95444343 @default.
- W2091293351 hasConcept C97029542 @default.
- W2091293351 hasConceptScore W2091293351C104317684 @default.
- W2091293351 hasConceptScore W2091293351C143065580 @default.
- W2091293351 hasConceptScore W2091293351C150109051 @default.
- W2091293351 hasConceptScore W2091293351C181199279 @default.
- W2091293351 hasConceptScore W2091293351C184235292 @default.
- W2091293351 hasConceptScore W2091293351C185592680 @default.
- W2091293351 hasConceptScore W2091293351C29688787 @default.
- W2091293351 hasConceptScore W2091293351C32619005 @default.
- W2091293351 hasConceptScore W2091293351C53345823 @default.
- W2091293351 hasConceptScore W2091293351C55493867 @default.
- W2091293351 hasConceptScore W2091293351C86803240 @default.
- W2091293351 hasConceptScore W2091293351C95444343 @default.
- W2091293351 hasConceptScore W2091293351C97029542 @default.
- W2091293351 hasIssue "46" @default.
- W2091293351 hasLocation W20912933511 @default.
- W2091293351 hasLocation W20912933512 @default.
- W2091293351 hasOpenAccess W2091293351 @default.
- W2091293351 hasPrimaryLocation W20912933511 @default.
- W2091293351 hasRelatedWork W1985385337 @default.
- W2091293351 hasRelatedWork W2011962611 @default.
- W2091293351 hasRelatedWork W2012014599 @default.
- W2091293351 hasRelatedWork W2042481436 @default.
- W2091293351 hasRelatedWork W2043312032 @default.
- W2091293351 hasRelatedWork W2085148012 @default.
- W2091293351 hasRelatedWork W2091293351 @default.
- W2091293351 hasRelatedWork W2142516871 @default.
- W2091293351 hasRelatedWork W2419260826 @default.
- W2091293351 hasRelatedWork W3005083546 @default.
- W2091293351 hasVolume "40" @default.
- W2091293351 isParatext "false" @default.
- W2091293351 isRetracted "false" @default.
- W2091293351 magId "2091293351" @default.
- W2091293351 workType "article" @default.