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- W2091299879 abstract "The gamma G/gamma A ratio was determined on 50 cord blood samples from normal newborns and in 28 beta-thalassemia homozygotes among the Lebanese population, using electrophoresis on acrylamide-bisacrylamide gels containing Triton X-100, urea, and 2-mercapto-ethanol. 2-Mercapto-ethanol was found to be the most effective reducer, giving good resolution and a clean background. As the concentration of Triton X-100 was critical in this study, a concentration of 500 microliters of Triton X-100/25 ml of reaction mixture was found to be optimal. In our 50 cord blood samples, the mean total Hb was 20 g/dl, with a range of 12-26. The mean fetal Hb (HbF) level was 64%, and the range 50-90%. The mean value for gamma G/gamma G + gamma A was 0.75. All beta-homozygotes had an elevated HbF, ranging from 7.7-99.3%, and their mean gamma G/gamma G + gamma A was 0.64. Only two out of 28 had gamma G/gamma G + gamma A ratio of 0.5; in the rest it was 0.60 or greater. This may be due to the greater resistance of erythrocytes with higher HbF and higher gamma G/gamma A destruction." @default.
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- W2091299879 date "1984-12-01" @default.
- W2091299879 modified "2023-09-25" @default.
- W2091299879 title "Determination of the γG/γA ratio in homozygous β-thalassemic patients among a Lebanese population" @default.
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- W2091299879 doi "https://doi.org/10.1016/0009-8981(84)90053-6" @default.
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